Contact Person

Dr. Jan Peychl
Head of the Light Microscopy Facility
Tel.: +49(351)210-2502
Email: lmf(at)mpi-cbg.de

Terms of Use

Light Microscopy Facility (LMF)
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Pfotenhauerstr. 108
01307 Dresden

General information: scientific core facilities at the MPI-CBG

The Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) in Dresden is one of 83 research institutes that collectively form the Max Planck Society for the Advancement of Science – Germany’s leading institution for basic research in the natural sciences, life sciences, social sciences, and the humanities - in the service of the general public. MPI-CBG is an international institute which conducts interdisciplinary multi-scale research in molecular life science and hosts independent research groups with expertise in molecular biology, physics and bioinformatics (www.mpi-cbg.de).

In order to support scientific goal of research groups and provide access to cutting edge techniques for members of the scientific community, MPI-CBG centralized broad range of key technologies and instrumentation in scientific core facilities headed by experts (http://mpi-cbg.de/services-facilities/core- facilities/). The core facilities at the MPI-CBG are independent of any research groups. They are open for MPI-CBG scientists as well as external academic and non-academic scientific organizations.

MPI-CBG is run according to full cost accounting principles. Access to the scientific core facilities is granted on a ‘recharge basis’ whereby the actual project-specific usage of the scientific core facility is recharged (for internal budgets) or invoiced (for third party-funded projects or external users). Importantly, all costs are categorized, monitored and accounted for such that ONLY project specific eligible costs and no general costs are included in the invoice charged to grants.

The following Terms of Use is binding for all employees and users of the Light Microscopy Facility (LMF) at MPI-CBG.

§1 Purpose and Aims

The mission of the Light Microscopy Facility (LMF) is to provide access to state of the art light microscopy techniques and instruments along with the vision towards future trends. LMF assists through consultation, training and project collaborations.

§2 Instrumentation and services

The LMF provides following services to research groups:

  • Project consultations and user training
    • planning of imaging experiments
    • consultation about new imaging technologies
    • user training on circa 25 advanced imaging systems of following categories:
  • Wide-field systems
    • Inverted and upright
    • CCD cameras for bright-field and fluorescence imaging
    • Systems for long-term time-lapse imaging of live cells
    • Total Internal Reflection Fluorescence (TIRF) system
    • Laser Capture Microdissection
    • Quantitative Phase Imaging
  • Confocal systems
    • Inverted and upright laser scanning confocal systems
    • Various laser and scan-head combinations
    • 2-photon systems
    • Spinning disc confocal systems
    • Systems for photomanipulation (laser ablation, photo-bleaching, photo-conversion)
  • Light sheet systems
    • for live and fixed cleared samples
  • Super resolution light microscopy
    • Stochastic Optical Reconstruction Microscopy (STORM)
    • Airy scan
    • SoRa – super resolution spinning disk system
  • Small equipment to support imaging experiments
    • Heating and cooling chambers
    • Objective heaters
    • Bookable objectives
    • Digital refractometer
  • Workstations for image processing and data analysis
    • Deconvolution
    • 3D volume rendering
    • Tracking
    • Processing of big image files

 

§3 Requirements and preparations

Users will be asked to fill in the “New user project questions sheet” in order to carefully define the imaging experiment with a testable hypothesis, to select appropriate imaging systems together with sample preparation, mounting, environmental control and to determine optimal imaging hardware settings to get the best imaging quality. “New user project questions sheet” is available upon request via e-mail: lmf(at)mpi-cbg.de.

All new users are requested to make an appointment with the member of the LMF team before booking the equipment for the first time. This appointment will be dedicated to discussion about imaging experiment and to selection of the most suitable imaging system.

Prior the training on imaging systems, all users are also required to take General Introduction to the LMF MPI-CBG which is provided in person by one of the staff members of the LMF MPI-CBG and which covers following topics:

LMF Booking Database

  • General structure of database, booking budgets, booking systems and assistance details

Booking Rules and User Responsibilities

  • All bookable items in the LMF database can be booked 16 days in advance
  • Booking of all items must truly reflect the reality, usage of imaging systems without booking is not allowed
  • For first time usage of all imaging systems LMF staff member MUST be booked together with the imaging system to provide user training and assistance
  • Typical length of user training on advanced imaging system is 2 x 3 hours, but might be extended further according to user needs and to the type of imaging system
  • On selected imaging systems which are heavily used booking budget of limited hours for which system can be booked might be applied
  • All imaging systems and also computers for image analysis MUST be booked for complete time which is needed for imaging and also for data storage and data transfer
  •  Cancelation of booking – the 24h rule
    • Users can delete their booking until 24 hours before the respective slot starts, without getting charged for it
    • If users decide that they cannot take an already booked slot only up to 24 hours before that slot starts, they can merely CANCEL it, i.e. their slot disappears out of the database, but the user will still get charged unless someone else takes the booked  slot
  • If a user is prevented to use an already booked slot which has not been canceled prior the 24 hours of the respective slot start, users are asked to do the following:
    • Cancel the slot in the booking database
    • Send an email to lmf-user(at)mpi-cbg.de, which contains following information: SIFFY (Slot Is Free For You), name of the system, date and time of the offered slot
  • Users are required to take care of the system after usage
    • At the end of the imaging sessions users MUST fill sign-up sheet which belongs to each imaging system
    • Users MUST clean the objective lenses that they used, and ALL the other lenses that are dirty
    • Users MUST also clean up all dirt and spilt immersion oil from the objective lens sides, the objective revolver, the stage, the stage insert and sample holder
    • Users MUST throw away all the waste that they have produced during their session
    • After system usage, users should log off of the used system and the respective software
    • Last user of the day is responsible to switch off the system, including all hardware components
    • In case that last user of the day forgets to switch the system off  and system will run overnight or over the weekend, LMF will charge the user as the system was used during this period of time

Safety and Waste

  • LMF is  certified as S1 area, usage of  S2 Genetically Modified Organisms (GMOs) it not allowed
  • Eating and drinking in whole LMF area is STRICTLY FORBIDDEN
  • Laser safety instructions will be provided to all users during the General Introduction to the Light Microscopy Facility
  • Waste: General (no S1 waste, no glass, no sharp objects); S1 (only for solid waste, user have to take a liquid waste back to the lab); Glass (yellow bins at systems are considered to be S1 glass waste); Sharp objects (special yellow bins at the systems for sharp objects only, are also considered to be S1 waste)

§4 Booking and recharge system

As per internal policy, internal users have a higher priority, however, the facility head or his deputy decides about he feasibility of the project before starting and may reject requests at his/her sole discretion.

Internal users receive a monthly report where the usage of the facility is summarized, whereas non-internal users receive an invoice on a quarterly basis.

The MPI-CBG has developed a unique online central User management system (‘Centralized Billing and Recharge System’ or ‘CBRS’) that is combined with a tracking and booking system, enables an accurate and transparent recharge. Three User categories (external, collaborator and internal) ensure that only eligible project specific costs are invoiced to grants while full costs are invoiced to external Users.

§5 Cost calculation

The MPI-CBG provides a high level of research services and facilities to the research groups, in the most cost-effective way and making the most efficient use of combined resources.

Users are charged a user fee according to the project specific usage of the resources and, depending on the User’s relationship with the MPI-CBG, will be invoiced on the basis of the three categories mentioned above:

“Internal users” are staff members of MPI-CBG, including employees, stipend holders, guest scientists / CBG-Fellows, and other research groups belonging to the Max Planck Society. Internal users are charged project specific personnel and consumables costs. Internal User prices are recharged to all grants awarded to the MPI-CBG and consist only of eligible costs. Importantly, such

‘Internal invoices’ represent a lower cost burden to the grant than is permitted by the funding agencies (DFG, BMBF, EU, ERC) since instrument depreciation is not included.

“Collaborators” are formally working together with an MPI-CBG research groups. The status “Collaborator” needs to be approved by the Chief Operating Officer ‘COO’ of the MPI-CBG. A Collaboration Agreement is mandatory for each ‘Collaborator’ User status and has to be supported by additional documentation, e.g. a joint grant application, jointly supervised student or postdoctoral fellow, or collaboration within a consortium. Collaborators are charged personnel, consumables and equipment depreciation.

“External users” are all users who are neither internal nor collaborators. They are charged full costs including personnel, consumables, equipment depreciation and indirect costs (administrative indirect costs related to personnel and building indirect costs related to building running costs).

Prices are adjusted on a yearly basis and are available to all registered users via LMF booking database: https://lmf.mpi-cbg.de/cgi-bin/index.py

§6 Intellectual property rights

MPI-CBG Services and Facilities provide their support and perform their services as a service provider and deliver the work results of such services without making claim to intellectual property pertaining to or related to the work results. As such and for the avoidance of doubt, all intellectual property rights pertaining to or related to the work results remain with and are owned by the User. Inventions and improvements or modifications to the technology platforms of the MPI-CBG Services and Facilities that do not incorporate the work results but that may have been discovered and/or reduced to practice during the course of performing the services belongs to the MPI-CBG and Max Planck Society and are covered by the respective Intellectual Property policy of the Max Planck Society.

§7 Acknowledgements

Work performed and data generated in the MPI-CBG core services and facilities requires the acknowledgement of the relevant service or facility if the work results of MPI-CBG Services and Facilities are used in scientific publications, papers, posters, presentations, grant applications, reports or other publications. The standard sentence for acknowledging the MPI-CBG Services and Facilities is: “We would like to thank the following Services and Facilities of the MPI-CBG for their support: Name of the Facility/Facilities”. A copy of the publication(s) acknowledging the support of MPI-CBG Services and Facilities shall be sent to the relevant MPI-CBG Services and Facilities Leader.

§8 User responsibilities

The User has to comply with the MPI-CBG "General Laboratory Regulations" and "Operating Instructions S1" and Operating Instructions S2" as well as any other relevant operating instructions that may apply to the scope of work executed at MPI-CBG.

The User has to comply with all instructions given by the MPI-CBG Service Leader or appointed supervisor within the facility and report any problems or safety relevant issues to the MPI-CBG supervisor.

Users are obliged to notify the Services and Facility Leader on malfunction or damage of the instruments without delay.

External Users are responsible for being insured by their employer or affiliated institute during their stay at MPI-CBG, and are liable for any damage caused by them during their stay.

§9 Data handling

Local data deletion policy

  • LMF generally allows users to store their data locally on the local hard drives of imaging systems up to 30 days
  • On specific imaging systems this data storage period might be shorter (14 days or 7 days)
  • Data older than specified period of time which is allowed for data storage on local drives will be deleted
  • LMF takes no responsibility for lost of data which is older than specified period for data storage on local drives

Central data storage

The central storage systems of MPI-CBG provides network protocol (SMB and NFS) access to storage and is connected to the internal network of the MPI- CBG. Access control to this storage is created on a project basis and access can be easily configured to be shared between service and facility members, researchers, groups, and any others with MPI-CBG accounts.

All data on the central storage is written to two tape copies in addition to being on disk and one copy is removed to offsite storage in order to assure that all data can be restored in case of a disaster. Projects when closed or published remain on tape and are archived according to good scientific practice. Access to the central storage systems is, in general, limited to internal users.

Internal users have free access to and utilize the central storage systems of the MPI-CBG for the data generated and used by the services and facilities.

Collaborators may request access to this central storage for the duration of the collaboration. This request is routed through the internal host lab collaborating with the external user who takes responsibility for the guest during their stay.

External users are requested to consult with the service or facility they are using to determine what type of data storage facilities they will require. The general rule is that data is stored within the MPI-CBG temporarily while processing or captured but should be moved to the home institution of the user as soon as is reasonably possible. The MPI-CBG provides tools to exchange data between the services and facilities and the home institution of external users. As the size and type of data varies significantly between the service and facilities the recommended method of transfer also varies. In the case of microscopy data external users are allowed to use the local storage attached to the individual microscopes for data storage for up to one month. After this time data is deleted automatically to make space for new data capture. In the case of exceptional data set size this time period may be reduced in order support the number of users using the systems.

The MPI-CBG IT Department is willing to work with other institutions when required to establish methods to temporarily store, move, and synchronize data to the external user’s institution if the project or usage of the facilities requires it.

§10 Problem reports, LMF hotline

Whenever user experiences a problem with the imaging system which he/she uses then is requested to report to the LMF either via lmf(at)mpi-cbg.de , or vial LMF booking database (Problems Section). For urgent troubleshooting cases LMF Hotline is available daily during workdays Monday to Friday from 9.00 – 17.00. Tel.: 00 49 351 210 2345.

§10 User community, mailing lists

All users are asked to become part of the LMF user community by subscribing to lmf-user(at)mpi-cbg.de mailing list and to specific mailing lists which are associated with the selected categories of advanced imaging systems. LMF staff uses these mailing lists to communicate important topics related to the operation of the advanced imaging systems.

§11 General advice for using the LMF MPI-CBG

Whenever user is not sure about LMF operating rules, usage of specific imaging systems, availability of different imaging technologies, or any other topics related to the operation of the LMF then it is ALWAYS highly recommended to contact LMF staff and to ask for individual consultation regarding the topic of interest.