Light Microscopy

MPI-CBG > Research > Scientific Cores & Support > Scientific Services > Light Microscopy > Laser Scanning Confocal Microscopes > CO3
OLYMPUS FLUOVIEW 3000 (INTERNAL NAME – CO3)

FEATURES

  • Olympus IX83 inverted motorized stand

  • Stage-top Z-piezo

  • Rapp OptoElectronic setup for additional scanner based photomanipulation

  • PicoQuant setup for FLIM, FRET, FLIM/FRET, and FCS measurements

  • Cage incubator for heating, CO2 and O2 level control

  • Suitable for slides, chambered slides, 35 mm dishes, and multi-well plates

  • Suitable for live samples, fixed samples and tissue sections

ILLUMINATION

  • Laser 405 nm

  • Laser 445 nm

  • Laser 488 nm

  • Laser 514 nm

  • Laser 561 nm

  • Laser 594 nm

  • Laser 640 nm

  • PicoQuant FLIM/FCS: laser lines: 440 nm, 485 nm, 510 nm, 560 nm, and 640 nm

  • Rapp OptoElectronic photomanipulation: 355 nm and 405 nm picosecond diode laser

  • More information about the laser power measurements can be found here

PHOTOMANIPULATION

  • All visible laser lines through main scanner

  • 355 and 405 nm laser lines through independent Rapp scanner 

FILTERS

  • Stand: 

    • Reflector revolver:

      • Epi1 (Deck 1): Mirror; Dapi (N2711900 U-FUW FL filter cube Exciter filter: 340-390 nm, beam splitter: 410 nm, barrier filter: 420 nm. Compatible with BX3-URA, BX3-RFAS and BX3-RFM); GFP (N2712800 U-FBNA FL filter cube Exciter filter: 470-495 nm. beam splitter: 505 nm. barrier filter: 510-550 nm. Compatible with BX3-URA, BX3-RFAS and BX3-RFM); RFP (N2713100 U-FGNA FL filter cube Exciter filter: 540-550 nm, beam splitter: 570 nm, barrier filter: 575-625 nm. Compatible with BX3-URA, BX3-RFAS and BX3-RFM); DIC; None; SP388; SP410

      • Epi2 (Deck 2): TB355/405/473

  • Scan head: variable detection of emission

    • MBS: DM405/488/561/640, DM405/488, DM405-445/514, DM405-445/514/594, DM405/488/594, BS10/90

    • SDM position 1 and 3: Mirror, Glass, SDM:400-470, SDM400-495, SDM400-540, SDM400-580

    • SDM position 2 and 4: Mirror, Glass, SDM:400-470, SDM400-495, SDM400-540, SDM400-580, SDM400-620

    • RAPP: ND  filters on manual slider 0/1/2/3

  • PicoQuant - manually switchable emission filters in front of APD detectors: Empty cube, 483/40 - 483/40, 550/49 - 550/49, 550/49 -483/40 (H488), 520/35 - 520/35, 600/50 - 600/50, 690/70 - 52-/35 (T635), 690/70 - 690/70, 600/50 - 520/35 (H560)

DETECTION

  • Olympus scan head: 4 GaAsP detectors, T-PMT

  • Picoquant: 2 APDs

OBJECTIVES

  • Olympus Plan Apo-N 2x (0.08 NA) Air

  • Olympus U Plan X-Apo 4x (0.16 NA) Air

  • Olympus U Plan X-Apo 10x (0.4 NA) Air

  • Olympus U ApoN340 20x (0.7 NA) Water

  • Olympus U Plan X-Apo 20x (0.8 NA) Air

  • Olympus U Plan S-Apo 30x (1.05 NA) Sil

  • Olympus U Plan X-Apo 40x (0.95 NA) Air

  • Olympus U ApoN340 40x (1.15 NA) Water

  • Olympus U Plan S-Apo 40x (1.25 NA) Sil

  • Olympus U ApoN340 40x (1.35 NA) Oil

  • Olympus Plan X-Apo 40x (1.4 NA) Oil

  • Olympus U Apo 40 x (0.65-1.35 NA) Oil 

  • Olympus U Plan S-Apo 60x (1.2 NA) Water

  • Olympus U Plan S-Apo 60x (1.3 NA) Sil

  • Olympus Plan Apo-N-SC2 60x (1.4 NA) Oil

  • Olympus U Plan X-Apo 60x (1.42 NA) Oil

SOFTWARE

  • For FV3000: 2.4.1.198 (Powered by H-PF version 2.7.2.435)

  • For Picoquant: Symphoquant 2.6, Build 5549

  • For RAPP: SysCon

  • For CellVivo -  Incubation/environment control system: CellVivo