FEATURES
Zeiss Axio Observer.Z1 inverted stand
Combined single- and multiphoton imaging with a tunable pulsed near-infra-red laser (Coherent Chameleon Vision II) for multiphoton excitation
Detection with two PMTs and one 32-channel GaAsP detector for spectral detection, five non-descanned detectors for multi-photon detection (two GaAsP), 2 T-PMT
Suitable for fixed samples, live samples and tissue sections
Photomanipulation (PA, FRAP, FLIP, FLAP, laser cutting)
Suitable for slides, chambered slides, 35 mm dishes, and multi-well plates
ILLUMINATION
Laser diode 405 nm
Laser Argon multiline 458, 488 and 514 nm
Laser DPSS 561 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
Laser multiphoton Coherent Chameleon Vision II 700-1064 nm
Fluorescence (metal halide, PRIOR, 200 W)
Transmitted light (halogen)
More information about the laser power measurements can be found here
PHOTOMANIPULATION
All visible laser lines through main scanner
Laser ablation with multiphoton laser
FILTERS
Stand:
Reflector revolver: GFP (Zeiss FS38, Ex BP470/40, BS495, EM BP525/50); Alexa 546 (Zeiss FS49, Ex G365, BS395, BP445/50), DAPI
Scan head:
MBS Twingate Visible branch: 458, 458/514, 458/561, 458/514/594, 488, 488/561, 488/594, 488/561/633, T80/R20, Plate
MBS Twingate Invisible branch: Plate, LP405, LP445, SP690, SP760, BP405-760, BP445-760, T80/R20
DETECTION
Single photon detection: 2 confocal PMTs, QUASAR detector with 32 channels, T-PMT, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting
2 photon detection: 5 non-descanned detectors for multi-photon detection (2 GaAsP)
OBJECTIVES
Zeiss Plan Apo 10x (0.45 NA) Air
Zeiss Plan Apo 20x (0.8 NA)
Zeiss LD LCI Plan-Apo 25x (0.8 NA) Oil/Glyc/Water DIC
Zeiss LD C-Apo 40x (1.1 NA) Water
Zeiss C-Apo 40x (1.2 NA) Water
Zeiss Plan Neofluar 40x (1.3 NA) Oil
Zeiss i LCI Plan Neofluar 63x (1.3 NA) Gly/Water DIC
Zeiss Plan Apo 63x (1.4 NA) Oil
SOFTWARE
Zeiss ZEN Black version 14.024.201