FEATURES
Nikon Eclipse Ti2 inverted motorized stand with hardware autofocus (PFS) and stage-top Z-piezo (travel range 200 μm)
Spinning disk confocal microscope for fast live cell imaging, especially C. elegans embryos
Scanner-based photo-manipulation (FRAP, PA, FLIP...)
Automated water immersion dispenser for the IR 60xAC WI (1.25 NA) objective
Suitable for imaging multi-well plates, 35 mm dishes, and chambered slides
ILLUMINATION
Laser diode 405 nm
Laser diode 488 nm
Laser DPSS 561 nm
Laser diode 594 nm
Laser diode 638 nm
Transmitted light (LED)
More information about the laser power measurements can be found here
PHOTOMANIPULATION
FRAP:
Scanner-based through upper back port
Laser 405, 488, 561 and 638 nm
FILTERS
Stand:
Reflector revolver (upper): pos1: empty, 405/488/561/640 FRAP mirror, pos2-6: empty
CSU-X1 scan head:
Dichromatic mirrors: Quad 405/488/561/640, 488SP, Triple 405/488/594
Emission filter wheels:
Emission filter wheels 1 and 2: pos1: Quad 440/521/607/700 BL; pos2: BP 447/60 BL; pos3: BP 525/50 BL; pos4: HC488 LP BL; pos5: ET Dualband Filter GFP/mCherry; pos6: Edge Basic LP 568; pos7: LP 594; pos8: HC617/73 BL; pos9: HC624/40 BL; pos10: 647 LP; pos11: LP ET 700; pos12: polarizer
DETECTION
Andor iXon DU-897U-CS0-#BV (SN X-13662)
Dexel (detector element) size: 16 μm
Hamamatsu ORCA-Fusion BT Digital CMOS (SN 500290)
Dexel (detector element) size: 6.5 μm
OBJECTIVES
Nikon CFI Plan Apochromat LambdaD 10x (0.45 NA) Air
Nikon CFI Plan Apochromat VC 20x (0.75 NA) Air
Nikon CFI Plan Apochromat IR 60xAC WI (1.25 NA) Water
Nikon SR HP Apochromat TIRF 100x (1.49 NA) Oil
More information about the pixel sizes and FOV can be found here
SCAN HEAD
Yokogawa CSU-X1 (speed 10000 rpm, pinhole diameter 50 μm, pinhole spacing 250 μm)
SOFTWARE
NIS-Elements 5.42.03 64-bit