FEATURES
Olympus IX83 inverted motorized stand with stage-top Z-piezo (travel range 400 μm)
Spinning disk confocal microscope for fast live cell, organoid, and large area imaging
Equipped with Borealis for even illumination
Photoactivation using 405 nm laser with high temporal and spatial resolution
Cage incubator for heating, CO2, and O2 level control
Suitable for imaging multi-well plates, 35 mm dishes, and (chambered) slides
ILLUMINATION
Laser diode 405 nm
Laser diode 445 nm
Laser DPSS 488 nm
Laser DPSS 514 nm
Laser DPSS 561 nm
Laser DPSS 633 nm
Laser DPSS 726 nm
Transmitted light (LED)
Fluorescence (LED)
More information about the laser power measurements can be found here
PHOTOMANIPULATION
Andor Mosaic
DMD-based coupled in through back port
Laser diode 405 nm (450 mW)
FILTERS
Stand:
Reflector revolver: DAPI set (Ex 377/50, DM 409, EM 447/60); GFP set (Ex 472/30, DM 495, EM 520/35); RFP set (Ex 562/40, DM 593, EM 624/40); CY5 set (Ex 628/40, DM 660, EM 692/40); Analyzer; DM for use with Mosaic
CSU-W1 scan head:
Dichromatic mirrors: Triple 445/514/640, Quad 405/488/561/640, Penta 405/488/561/640/720
Dichromatic beamsplitter: 561LP, 514LP, glass
Emission filter wheels:
Emission filter wheel 1 (primary camera): pos1: RazorEdge LP Edge Basic Filter 568; pos2: BP 525/50 BL HC; pos3: BP 617/73 BL HC; pos4: BP 607/36 BL HC; pos5: BP 685/40 BL HC; pos6: BP 785/62 BL HC; pos7: polarizer; pos8: RazorEdge LP Edge Basic Filter 514; pos9: BP 542/27 BL HC; pos10: BP 452/45 BL HC
Emission filter wheel 2 (secondary camera): pos1: BP 465/30 BL HC; pos2: BP 514/30 BL HC; pos3: BP 525/50 BL HC; pos4: BP 479/40 BL HC; pos5: polarizer; pos6–10: empty
DETECTION
2 Andor iXon 888 Ultra EMCCD cameras (with etaloning suppression): primary: SN DU888_BV (SN X-10332), secondary: SN DU888_BV (SN X-10396)
Dexel (detector element) size: 13 μm
OBJECTIVES
Olympus U Plan SApo 10x (0.4 NA) Air
Olympus U Plan Fluar 20x (0.5 NA) Air
Olympus U Plan SApo 30x (1.05 NA) Sil
Olympus U Plan SApo 40x (1.25 NA) Sil
Olympus U Plan SApo 60x (1.3 NA) Sil
Olympus U Plan SApo 100x (1.4 NA) Oil
Olympus U Plan SApo 100x (1.34 NA) Sil
Olympus U ApoN 150x (1.45 NA) TIRFM
More information about the pixel sizes and FOV can be found here
SCAN HEAD
Yokogawa CSU-W1 (4000 rpm with two pinhole disks: 25 μm and 50 μm diameter, respectively; pinhole spacing 500 μm for both disks) with Borealis illumination delivery
SOFTWARE
Andor iQ 3.6