FEATURES
Olympus IX83 inverted motorized stand with hardware autofocus (ZDC2) and stage-top Z-piezo (travel range 400 μm)
Spinning disk confocal microscope for fast live cell, organoid, and large area imaging with high spatial resolution (down to 120 nm in XY) via SORA disk
Photoactivation using 405 nm laser with high temporal and spatial resolution.
Rapp Optoelectronics FLUCS photomanipulation system
Cage incubator for heating, CO2, and O2 level control
Suitable for imaging multi-well plates, 35 mm dishes, and chambered slides
ILLUMINATION
Laser diode 405 n
Laser diode 445 nm
Laser DPSS 488 nm
Laser DPSS 515 nm
Laser DPSS 561 nm
Laser DPSS 640 nm
Transmitted light (LED)
Fluorescence (LED: CoolLED pE-4000 with 16 lines: pE-4000 | LED Light Sources | CoolLED LED Illumination System)
More information about the laser power measurements can be found here
PHOTOMANIPULATION
RAPP Optoelectronic FLUCS:
Scanner-based coupled in through back port
1455 nm for focus-light induced cytoplasmic streaming (FLUCS)
FRAP:
Scanner-based through right side port
Laser diode 405 nm
FILTERS
Stand:
Reflector revolver: pos1: STLP-FLUCS-RAPP; pos2: U-FUNA (Ex 360-370, DM 410, EM 420-460); pos3: U-FBNA (Ex 470-495, DM 505, EM 510-550); pos4: U-FGW (Ex 530-550, DM 570, EM 575IF); pos5: none; pos6: 405_LED_FR; pos7: DCSP1260-FLUCS-AHF; pos8: IX3-FDICT (DIC analyzer)
Reflector revolver: pos1: STLP-FLUCS-RAPP; pos2: U-FUNA (Ex 360-370, DM 410, EM 420-460); pos3: U-FBNA (Ex 470-495, DM 505, EM 510-550); pos4: U-FGW (Ex 530-550, DM 570, EM 575IF); pos5: none; pos6: TxRed ET Filterset (Ex 560/40, DM 585, EM 630/75, installed 4th April 2023); pos7: Cy5 ET Filterset (Ex 620/60, DM 660, EM 700/75, installed 4th April 2023); pos8: IX3-FDICT (DIC analyzer)
CSU-W scan head:
Dichromatic mirrors: Triple 445/514/640, Quad 405/488/561/640, mirror
Dichromatic beamsplitter: 561LP, 514LP, glass
Emission filter wheels:
Emission filter wheel 1: pos1: BP 447/60 BL HC; pos2: BP 525/50 BL HC; pos3: BP 617/73 BL HC; pos4: BP 685/40 BL HC; pos5: BP 482/35 BL HC; pos6: BP 578/105 BL HC; pos7: BP 607/36 BL HC; pos8: LP Edge Basic Filter 647; pos 9: LP Edge Basic Filter 488; pos10: LP Edge Basic LP 568
Emission filter wheel 2: pos1: BP 447/60 BL HC; pos2: BP 525/50 BL HC; pos 3: BP 482/35 BL HC; pos4: BP 542/27 BL HC; pos5: BP 514/30 BL HC; pos 6: polarizer
DETECTION
2 Hamamatsu ORCA-Fusion BT Digital CMOS cameras (SN 500674 and SN 500670)
Dexel (detector element) size: 6.5 μm
OBJECTIVES
Olympus U Plan SApo 4x (0.16 NA) Air
Olympus U Plan SApo 10x (0.4 NA) Air
Olympus U Plan XApo 20x (0.8 NA) Air
Olympus U Plan SApo 20x (0.85 NA) Oil
Olympus U Plan SApo IR 30x (1.05 NA) Sil
Olympus U Plan CN 40x (0.65 NA) Air
Olympus U Plan SApo 40x (0.95 NA) Air
Olympus U Plan 60x (1.2 NA) Water
Olympus U Plan SApo 60x (1.3 NA) Sil
Olympus U Plan SApo 100x (1.35 NA) Sil
Olympus U-ApoN 100x (1.49 NA) Oil
More information about the pixel sizes and FOV can be found here
SCAN HEAD
Yokogawa CSU-W1 (4000 rpm with two disks: 50 μm and SORA for superresolution)
SOFTWARE
Olympus cellSens 4.1
For RAPP: SysCon2