Light Microscopy

Olympus IX83 inverted motorized stand with hardware autofocus (ZDC2) and stage-top Z-piezo (travel range 500 μm) 

Spinning disk confocal microscope for fast live cell, organoid, and large area imaging with high spatial resolution (down to 120 nm in XY) via SORA disk

Scanner-based photo-manipulation (FRAP, PA, FLIP...) with high temporal and spatial resolution

Scanner-based IR cutter system

Cage incubator for heating, CO₂, and O₂ level control

Suitable for imaging multi-well plates, 35 mm dishes, and chambered slides

Laser diode 405 nm

Laser diode 445 nm

Laser DPSS 488 nm

Laser DPSS 515 nm

Laser DPSS 561 nm

Laser DPSS 640 nm

Transmitted light (LED)

Fluorescence (LED: CoolLED pE-4000 with 16 lines: pE-4000 | LED Light Sources | CoolLED LED Illumination System)

More information about the laser power measurements can be found here

RAPP Optoelectronic IR-cutter:

• Scanner-based coupled in through back port

• 1040 nm IR laser from SPARK

RAPP Optoelectronic FRAP:

• Scanner-based coupled in through back port

• 4 Laser diodes 405 nm, 473 nm, 552 nm and 633 nm

Stand: 

• Reflector revolver 1:

  • pos1: Free

  • pos2: SP-775 (for IR Cutter)

  • pos3: FRAP 30/60

  • pos4: Free

  • pos5: Free

  • Pos6: Free

  • Pos7: Free

  • Pos8: Free

• Reflector revolver 2:

  • pos1: DAPI (Ex 360-370, DM 410, EM 420-460)

  • pos2: FITC (Ex 470-495, DM 505, EM 510-550)

  • pos3: Cy3 

  • pos4: mCherry (Ex 530-550, DM 570, EM 575IF)

  • pos5: Cy5 (Ex 620/60, DM 660, EM 700/75)

  • pos6: IX3-FDICT (DIC analyzer)

  • pos7: Free

  • pos8: Free

CSU-W scan head:

• Dichromatic mirrors:

  • Quad 405/488/561/640

  • Triple 445/514/640

  • Full mirror

• Dichromatic beamsplitter:

  • 561 LP

  • 514 LP

  • Glass plate

Emission filter wheels:

• Emission filter wheel 1:

  • pos1: BP 447/60 BL HC (DAPI)

  • pos2: BP 525/50 BL HC (GFP, Alexa 488)

  • pos3: BP 617/73 BL HC (RFP, Cherry wide)

  • pos4: BP 685/40 BL HC (Cy5, SIR)

  • pos5: BP 482/35 BL HC (CFP)

  • pos6: BP 578/105 BL HC (YFP, Venus)

  • pos7: BP 607/36 BL HC (RFP, Cherry narrow)

  • pos8: LP Edge Basic 647 (BLP01-647R-25)

  • pos 9: LP Edge Basic 488 (BLP01-488R-25)

  • pos10: LP Edge Basic 561 (BLP01-568R-25)

• Emission filter wheel 2:

  • pos1: BP 447/60 BL HC (DAPI)

  • pos2: BP 525/50 BL HC (GFP, Alexa 488)

  • pos 3: BP 482/35 BL HC (CFP)

  • pos4: BP 542/30 BL HC (YFP, Venus)

  • pos5: BP 514/30 BL HC (narrow GFP)

  • pos 6: Polarizer 

2 Hamamatsu ORCA-Fusion BT Digital CMOS cameras (SN 502545 and SN 502403)

• Dexel (detector element) size: 6.5 μm

Olympus U Plan XApo 4x (0.16 NA) Air

Olympus U Plan XApo 10x (0.4 NA) Air

Olympus U Plan XApo 20x (0.8 NA) Air

Olympus LU Plan Apo 25x (0.85 NA) Silicone gel/ Water

Olympus U Plan SApo IR 30x (1.05 NA) Sil

Olympus U Plan XApo 40x (0.95 NA) Air

Olympus U Apo N340 40x (1.15 NA) Water

Olympus U Plan SApo 40x (1.25 NA) Sil

Olympus U Plan SApo 1600 60x (1.2 NA) Water

Olympus U Plan SApo 60x (1.3 NA) Sil

Olympus U Plan XApo 60x (1.42 NA) Oil

Olympus U Plan SApo 100x (1.35 NA) Sil

Olympus U Plan Apo HR 100x (1.5 NA) Oil

Olympus U Plan N TIRF 150x (1.45 NA) Oil

More information about the pixel sizes and FOV can be found here

Yokogawa CSU-W1 (4000 rpm with two disks: 50 μm and SORA for superresolution) 

Olympus cellSens 4.3.1

Olympus CellVivo (incubation)

For RAPP: SysCon2