FEATURES
Zeiss Axio Examiner.Z1 upright stand
Combined single- and multiphoton imaging with a tunable pulsed near-infra-red laser (Coherent Chameleon Vision II) for multiphoton excitation
Detection with two PMTs and one 32-channel GaAsP detector for spectral detection in the scanhead, four GaAsP non-descanned detectors for multiphoton detection, three transmitted light detectors (two for multiphoton, one for single-photon)
Motorized stage: suitable for 2D images, z-stacks, timelapses, bleaching and tile scans
Photomanipulation (PA, FRAP, FLIP, FLAP, laser cutting)
Suitable for live samples, fixed samples, and tissue sections
Suitable for slides, and sample carriers, compatible with dipping lenses
ILLUMINATION
Laser diode 405 nm
Laser argon multiline 458, 488 and 514 nm
Laser DPSS 561 nm
Laser HeNe 594 nm
Laser HeNe 633 nm
Laser multiphoton Coherent Chameleon Vision II, 700-1064 nm
Fluorescence (metal halide, HXP, 120 W)
Transmitted light (halogen)
More information about the laser power measurements can be found here
PHOTOMANIPULATION
All visible laser lines through main scanner
Laser ablation with multiphoton laser
FILTERS
Stand:
Reflector revolver: Cy3.5 (Zeiss set 49); DAPI (Ex G365, BS FT 395, EM BP 445/50); GFP (Zeiss set 38); Endow GFP shift free (E) (EX BP 470/40, BS FT 495, EM BP 525/50); HQ Texas Red (Zeiss set 45) shift free (E) (EX BP 560/40, BS FT 585, EM BP 630/75)
Scan head:
MBS Twingate visible branch: 458/514/561/633, 458/514, 458/561, 458/514/594, 488, 488/561, 488/594, 488/561/633, T80/R20, Plate
MBS Twingate invisible branch: Plate, LP405, LP445, SP690, SP760, BP 405-760, BP 445-760, T80/R20
DETECTION
Single photon detection: 2 confocal PMTs with enhanced detection in the red range, GaAsP spectral detector with 32 channels, T-PMT, spectral prism sliders for emission detection range selection, common pinhole changeable for each detector setting.
2 photon detection: 4 non-descanned GaAsP detectors (2 BiG units) in reflected direction, 2 non-descanned PMTs (1 NDD module) in transmitted direction
OBJECTIVES
Zeiss Plan Apo 10x (0.45 NA) Air
Zeiss Clr Plan Apo 10x (0.5 NA) Scale
Zeiss W Plan Apo 20x (1.0 NA) DIC
Zeiss Clr Plan Neofluar 20x (1.0 NA) Korr Clarity
Zeiss LD Plan Apo 20x (1.0 NA) Korr Scale
Zeiss LD LCI Plan Apo 25x (0.8 NA) Oil/Glyc/Water DIC
Zeiss C-Apochromat 40x (1.2 NA) Water
Zeiss LCI plan-neofluar 63x/1.3 DIC Imm Korr
Zeiss C-Plan Apochromat 40x/1.3 Oil DIC for clearing
Zeiss Plan Neofluar 40x/1.3 Oil
Zeiss PlanApo 10X/0.5
Zeiss Plan-Apochromat 63X/1.4 Oil
Zeiss W Plan Apochromat 40x/1.0 DIC
SOFTWARE
Zeiss ZEN 2011 SP7 FP3 (black) version 14.0.25.201