B. Küster, A.Shevchenko, M. Mann
Mass spectrometry of proteolysis-derived peptides for protein identification.
In Proteolytic enzymes: A practical approach (Ed. R.Beynon, J.S.Bond), Oxford University press, Oxford, New York 2000, pp.149-185

S. Aigner, J. Lingner, K.J.Goodrich, C. A. Grosshans, A. Shevchenko, M. Mann, T.R.Cech
Euplotes telomerase contains an La motif protein produced by apparent translational frameshifting.
(2000) EMBO J. 19, 6230-6239

R.Kumar, D. M. Reynolds, A. Shevchenko, A. Shevchenko, S. D. Goldstone, S. Dalton
Forkhead transcription factors, Fkh1p and Fkh2p, collaborate with Mcm1p to control transcription required for M-phase
(2000) Curr. Biol. 10, 896-906

B. Charroux, L. Pellizzoni, R.A. Perkinson, J. Yong, A. Shevchenko, M. Mann, G. Dreyfuss
Gemin4: a novel component of the SMN complex that is found in both gems and nucleoli.
(2000) J. Cell Biol. 148, 1177-1186

A.Shevchenko*, I. Chernushevich, M. Wilm, M. Mann
De Novo peptide sequencing by nanoelectrospray tandem mass spectrometry using triple quadrupole and quadrupole - time-of-flight instruments.
Methods Mol Biol 146, 1- 16

R. Ciosk, M. Shirayama, A. Shevchenko, T. Tanaka, A. Toth, A. Shevchenko, K. Nasmyth (2000)
Cohesin's binding to chromosomes depends on a specialized loading factor.
Mol. Cell. 5, 243-254

Brdicka T, Pavlistova D, Leo A, Bruyns E, Korinek V, Angelisova P, Scherer J, Shevchenko A, Hilgert I, Cerny J, Drbal K, Kuramitsu Y, Kornacker B, Shevchenko A., Horejsi V, Schraven B

Phosphoprotein associated with glycosphingolipid-enriched microdomains (PAG), a novel ubiquitously expressed transmembrane adaptor protein, binds the protein tyrosine kinase Csk and is involved in regulation of T cell activation.

(2000) J. Exp Med 191, 1591-1604

M.Bagnat, S. Keranen, A. Shevchenko, A. Shevchenko, K. Simons

Lipid rafts function in biosynthetic delivery of proteins to the cell surface in yeast.

(2000) Proc. Natl. Acad. Sci. USA. 97, 3254-3259

Shevchenko A*, Loboda A., Shevchenko A., Ens W, Standing K.

MALDI -Quadrupole Time - Of -Flight mass spectrometry: a powerful tool in proteomic research.

(2000) Anal. Chem.,72 2132-2141

M. Schaefer, A. Shevchenko, A. Shevchenko, J.A. Knoblich

A protein complex containing Inscuteable and the Ga binding protein Pins orients asymmetric cell divisions in Drosophila.

(2000) Curr. Biol. 10, 353-362

Schramm C, Elliott S, Shevchenko A, Shevchenko A., Schiebel E.

The Bbp1p-Mps2p complex connects the SPB to the nuclear envelope and is essential for SPB duplication.

(2000) EMBO J. 19, 421-433.

Lechler T., Shevchenko A., Shevchenko A., Li R.

Direct involvement of yeast type I myosins in Cdc42-dependent actin polymerization.

(2000) J. Cell Biol. 148, 363-373


Charroux B., Pellizzoni L., Perkinson R.A., Shevchenko A., Mann M., Dreyfuss G.

Gemin3: a novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems.

(1999) J. Cell Biol. 147, 1181-1193.

Hensel F, Hermann R, Schubert C, Abe N, Schmidt K, Franke A, Shevchenko A, Mann M, Muller-Hermelink H.K., Vollmers H.P.

Characterization of glycosylphosphatidylinositol-linked molecule CD55/decay-accelerating factor as the receptor for antibody SC-1-induced apoptosis.

(1999) Cancer Res. 59, 5299-5306

Willems A.R., Goh Th., Taylor L., Chernushevich I., Shevchenko A., Tyers M.

SCF ubiquitin protein ligases and phosphorylation-dependent proteolyis.

(1999) Phil. Trans. R. Soc. Lond. B. 354, 1533-1550

A.Shevchenko*, W. Zachariae, A. Shevchenko

A strategy for the characterization of protein interaction networks by mass spectrometry.

(1999) Biochem. Soc. Trans. 27, 549-554

A. Shevchenko, M. Mann

Deciphering functionally important multiprotein complexes by mass spectrometry

(1999) In “Mass Spectrometry in biology and medicine” (Eds. A. Burlingame, S.A. Carr, M.A. Baldwin), Humana Press, Totowa NJ, 237-269

Perrot M., Sagliocco F., Mini Th., Monribot Ch., Schneider U., Shevchenko A., Mann M., Jeno P., Boucherie H.

Two-dimensional gel protein database of Saccharomyces cerevisiae

(update 1999). Electrophoresis 20, 2280-2298

Seol JH, Feldman RM, Zachariae W, Shevchenko A, Correll CC, Lyapina S, Chi Y, Galova M, Claypool J, Sandmeyer S, Nasmyth K, Shevchenko A., Deshaies RJ.

Cdc53/cullin and the essential Hrt1 RING-H2 subunit of SCF define a ubiquitin ligase module that activates the E2 enzyme Cdc34.

(1999) Genes Dev. 13, 1614-1626

Pappa A, Seferiadis K, Fotsis T, Shevchenko A, Marselos M, Tsolas O, E Messinis I

Purification of a candidate gonadotrophin surge attenuating factor from human follicular fluid.

(1999) Hum Reprod. 14, 1449-1456

Shou W, Seol JH, Shevchenko A, Baskerville C, Moazed D, Chen ZW, Jang J, Shevchenko A, Charbonneau H, Deshaies RJ.

Exit from mitosis is triggered by Tem1-dependent release of the protein phosphatase Cdc14 from nucleolar RENT complex.

(1999) Cell 97, 233-244

Mercurio F, Murray BW, Shevchenko A, Bennett BL, Young DB, Li JW, Pascual G, Motiwala A, Zhu H, Mann M, Manning AM

I B kinase (IKK)-associated protein 1, a common component of the heterogeneous IKK complex.

(1999) Mol Cell Biol. 19, 1526-1538

Jensen ON, Wilm M, Shevchenko A, Mann M

Sample preparation methods for mass spectrometric peptide mapping directly from 2-DE gels.

(1999) Methods Mol Biol. 112, 513-530

Jensen ON, Wilm M, Shevchenko A, Mann M

Peptide sequencing of 2-DE gel-isolated proteins by nanoelectrospray tandem mass spectrometry.

(1999) Methods Mol Biol. 112, 571-588

Marie-Cardine A, Kirchgessner H, Bruyns E, Shevchenko A, Mann M, Autschbach F, Ratnofsky S, Meuer S, Schraven B

SHP2-interacting transmembrane adaptor protein (SIT), a novel disulfide-linked dimer regulating human T Cell activation.

(1999) J Exp Med. 189, 1181-1194

K. Siegers, Th. Waldmann, M.R.Leroux, K. Grein, A. Shevchenko, E.Schiebel, F.U. Hartl

Compartmentation of protein folding in vivo: sequestration of non-native polypeptide by the chaperonine - GimC system.

(1999) EMBO J. 18, 75-84


K. Takase, Ya. Okazaki, K. Wakizaka, A. Shevchenko, M.Mann, T. Saito

Molecular cloning of pTAC12: An alternative splicing product of CD3g chain as a component of the pre-TCR complex

(1998) J. Biol. Chem. 273, 30675-30679

R. - H. Chen, A. Shevchenko, M. Mann, A.W. Murray

Spindle checkpoint protein xmad1 recruits xmad2 to unattached kinetochores

(1998) J. Cell Biol.143, 283-295

E. Bruyns, A. Marie-Cardine, H. Kirchgessner, K. Sagolla, A. Shevchenko, M. Mann, F. Autschbach, A. Bensussan, S. Meuer, B. Schraven. T

Cell Receptor (TCR) interacting molecule (TRIM), a novel disulfide linked dimer associated with the TCR/CD3/x- complex, recruits intracellular signaling proteins to the plasma membrane.

(1998) J. Exp. Med. 188, 561-575

R. Ciosk, Ch. Michaelis, W. Zachariae, A.Shevchenko, M. Mann, K. Nasmyth

An ESP1/PDS1 complex regulates loss of sister chromatid cohesion at the metaphase to anaphase transition in yeast.

(1998) Cell 93, 1067-1076

W. Zachariae, A. Shevchenko, P Andrews, R. Ciosk, M. Galova, M. Stark, M. Mann, K. Nasmyth

Mass spectrometric analysis of the Anaphase Promoting Complex from budding yeast: Identification of a subunit related to cullins.

(1998) Science 279, 1216-1219


F.Severin, N.A.Shanina, A.Shevchenko, O.B.Solovyanova, V.V.Koretsky, E.S.Nadezhdina.

A major 170 kDa protein associated with bovine adrenal medulla microtubules: a member of the centrosomin family?

(1997) FEBS Letters 420, 125-128

A.Shevchenko, P.Keller, P.Scheiffele, M.Mann and K.Simons

Identification of components of trans-Golgi network-derived transport vesicles and detergent-insoluble complexes by nanoelectrospray tandem mass spectrometry.

(1997) Electrophoresis 18, 2591-2600

F.Mercurio, H. Zhu, B.W.Murray, A.Shevchenko, B.Bennett, J.W.Li, M.Barbosa, M.Mann, A.Manning, A.Rao

IKK-1 and IKK-2: Cytokine-activated IkB kinases essential for NF-kB activation.

(1997) Science 278, 860-866

Ph. Mitchell, E. Petfalski, A. Shevchenko, M. Mann D. Tollervey

The exosome: A conserved eukaryotic RNA processing complex containing multiple 3'-> 5' exoribonucleases.

(1997) Cell 91, 457-466

P.Grandi, T.Dang, N.Pante, A.Shevchenko, M.Mann, D.Forbes, E.Hurt

Nup93, a vertebrate homolog of Yeast Nic96p, forms a complex with a novel 205 kDa protein and is required for correct nuclear pore assembly.

(1997) Mol.Cell.Biol. 8, 2017-2038

K.M. McNagny, I.Pettersson, F. Rossi, A.Shevchenko, M.Mann, T. Graf

Thrombomucin, a novel cell surface protein that defines thrombocytes and multipotent hematopoietic progenitors.

(1997) J Cell Biol 138, 1395-1407

Mirgorodskaya O.A, Saveleva N.V., Simankova A.N., Shevchenko A.A., Aleksandrov S.L.

The digestion of luliberine analogues containing D-alanine residue by human gastric juice.

(1997) Russian J. Bioorgan. Chem. 23, 555-559

Mirgorodskaya O.A, Kazanina G.A, Mirgorodskaya E.P, Shevchenko A.A, Mal'tsev KV, Miroshnikov AI, Roepstorff P

Proteolysis of human proinsulin catalysed by native, modified, and immobilized trypsins.

(1997) Russian J. Bioorgan. Chem. 23, 82-88

A.Shevchenko, I.Chernushevich, W.Ens, K.G.Standing, B.Thomson, M.Wilm M.Mann

Rapid “De Novo” peptide sequencing by a combination of nanoelectrospray, isotopic labeling and a quadrupole / time-of-flight mass spectrometer.

(1997) Rapid Commun. Mass Spectrometry 11, 1015-1024

Shevchenko, A, Wilm, M., Mann, M.

Peptide sequencing by mass spectrometry for homology searches and cloning of genes.

(1997) J. Protein Chem. 16, 481 - 490

T.Kawata, A. Shevchenko, M. Fukuzawa, K. A. Jermyn, N. F. Totty, N. V. Zhukovskaya, A.Sterling, M. Mann, and J. G. Williams

SH2 signaling in a lower eukaryote: a STAT protein that regulates stalk cell differentiation in Dictyostelium.

(1997) Cell 89, 909-916

Medema, J.P. , Scaffidi, C., Kischkel, F.C., Shevchenko, A., Mann, M., Krammer, P.H., Peter, M.E.

FLICE is activated by association with the CD95 death-inducing signaling complex (DISC)

(1997) EMBO J. 16 2794 - 2804

Lingner, J., Hughes, T.R., Shevchenko, A., Mann, M., Lundblad, V., and Cech, T.R.

Reverse transcriptase motifs in the catalytic subunit of telomerase.

(1997)Science 276, 561 - 567

Jensen, O.N., Shevchenko, A. and Mann, M.

Protein analysis by mass spectrometry.

In: Protein structure - A Practical Approach, 2nd Edition (T. E. Creighton, Ed.). IRL Press, Oxford (1997) pp. 29 - 57


Shevchenko, A., Jensen, O.N., Podtelejnikov, A.V., Sagliocco, F., Wilm, M., Vorm, O., Mortensen, P., Shevchenko, An., Boucherie, H., Mann,M.

Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels.

(1996) Proc. Natl. Acad. Sci. USA 93 14440 - 14445

Shevchenko, A., Wilm, M., Vorm, O.,Jensen, O.N., Podtelejnikov, A.V., Neubauer, G., Shevchenko, A., Mortensen, P., Mann, M.

A strategy for identifying gel separated proteins in sequence databases by mass spectrometry alone.

(1996) Biochem. Soc. Trans. 28, 893 - 896

O.N. Jensen, T. Houthaeve, A. Shevchenko, S. Cudmore, T. Ashford, M. Mann, G. Griffiths, J. Kijnse Locker

Identification of the major membrane and core proteins of vaccinia virus by two -dimensional electrophoresis.

(1996) J.Virology 70, 7485-7497

G. Simos, A. Segref, F. Fasiolo, K. Hellmuth, A. Shevchenko, M. Mann, E.C. Hurt

The yeast protein Arc1p binds to tRNA and functions as a cofactor for methionyl- and glutamyl - tRNA synthetases.

(1996) EMBO J. 15, 5437 - 5448

M. Muzio#, A. M. Chinnaiyan#, F.C.Kischkel#, K. O’Rourke#, A.Shevchenko#, J. Ni#, C. Scaffidi, J. Bretz, M. Zhang, R. Gentz, M.Mann, P H.Kramer, M.Peter, V.M.Dixit

FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signalling complex.

(1996) Cell 85, 817-827

(# - equal contribution of these authors)

A. Shevchenko, M. Wilm, O. Vorm, M. Mann

Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels.

(1996) Anal. Chem. 68, 850-858

M. Wilm#, A. Shevchenko#, T. Houthaeve, S. Breit, L. Schweigerer, Th. Fotsis , M.Mann

Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry.

(1996) Nature 379, 466-469

(#- equal contribution of MW and AS)


E.P.Mirgorodskaya, O.A.Mirgorodskaya, S.V.Dobretsov, A.A.Shevchenko, A.F.Dodonov, V.I.Kozlovskiy, V.V.Raznikov

Electrospray mass spectrometric study of melittin trypsinolysis by a kinetic approach.

(1995) Anal.Chem. 67, 2864-2869


O.A.Mirgorodskaya, A.A.Shevchenko, A.F.Dodonov, I.V.Chernushevich, A.I.Miroshnikov

Electrospray ionization time-of-flight mass spectrometry in protein chemistry.

(1994) Anal. Chem. 66, 99-107


O.A.Mirgorodskaya, A.A.Shevchenko, Kamal Omer M.A.A., I.V.Chernushevich, Tz.A.Egorov, A.X.Musoliamov, V.N.Kokryakov, O.V.Shamova

Primary structure of three cationic peptides from porcine neutrophils. Sequence determination by the combined usage of electrospray ionization mass spectrometry and Edman degradation.

(1993) FEBS Lett. 330, 339-342

V.N.Kokryakov, S.S.L.Harvig, E.A.Panyutich, A.A.Shevchenko, G.M.Aleshina, O.V.Shamova, H.A.Korneva, R.I.Lehrer

Protegrins: leukocyte antimicrobial peptides that combine features of corticostatic defensins and tachyplesins.

FEBS Lett. 327, 231-236


O.A.Mirgorodskaya, A.A.Shevchenko

Bradykinin degradation pathways in human blood plasma.

FEBS Lett. 307, 263-266

1991 - 1987

V.P.Goncharova, M.Yu.Eropkin, N.I. Chalisova, G.N.Akoev, E.M.Eropkina, A.A.Shevchenko

A comparative analysis of biological activity of the acid-soluble brain protein and fibroblast growth factor.

(1991) Tsitologiya 33, 67-75. (in Russian)

Mirgorodskaya O.A., Podtelezhnikov A.V., Shevchenko A.A.

The use of high performance liquid chromatography - SIE AP mass spectrometry for the study of peptide degradation in human blood plasma and serum.

(1989) Biokhimiya 54, 1186-1192. (in Russian)

English version: (1990) Biochemistry. English Translations from Biokhimiya 55, 973-978.

Ryazanov E.M., Tennikov M.B., Shevchenko A.A.

Quantitative analysis of antipyrine and its metabolites in rat urine by high performance liquid chromatography - SIE AP mass spectrometry.

(1989) Khimiko-Farmatsevticheskii Zhurnal, 103-105. (in Russian)

Tennikov M.B., Shevchenko A.A.

Reversed-phase isocratic high performance liquid chromatography of the substances containing strongly basic groups.

(1988) Zhurnal Analyticheskoi Khimii 44 , 952-954 (in Russian)

Mirgorodskaya O.A., Poletaeva L.V., Shevchenko A.A.

Determination of cysteine in proteins with Ellman's reagent by a reversed phase HPLC.

(1987) Zhurnal Analyticheskoi Khimii 43, 1885-1890 (in Russian)