Andrej Shevchenko Group

MPI-CBG > Research > Research Groups > Current Groups > Andrej Shevchenko > Projects > Clinical lipidomics

Clinical lipidomics

We apply shotgun mass spectrometry technology to analyze the lipidome of samples obtained from patients with various health conditions (spreading from healthy to cancer). Our translational clinical lipidomics projects focus on the full lipidome quantification of the following organs and tissues:

  • Dr. Yuting Wang studies colorectal cancer (CRC). CRC is one of the most common cancers worldwide. Solid cancers show broad metabolic alterations, which contribute to sustained tumor proliferation. To understand the lipid metabolism in CRC, we performed a comprehensive and quantitative shotgun lipidomics using CRC tissue and matched adjacent normal tissue from the same CRC patients. This project is performed in collaboration with Dr. Sebastian Zeissig from the Center for Regenerative Therapies Dresden (Zeissig lab).
  • Dr. Oskar Knittlefelder studies pancreas. To understand the metabolic changes in pancreatic islets of diabetic patients (from healthy to diseased) we apply our established shotgun LCM (link to project page). Using the inherent autofluorescene of human pancreatic islets we will decipher changes in the lipidome. This project is performed in collaboration with Dr. Michele Solimena from the Paul Langerhans Institute in Dresden (Solimena Lab).
  • Dr. Olga Vvedenskaya and Dr. Oskar Knittelfelder study liver pathological conditions, such as non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH). NAFLD is a liver disease characterized by the lipid accumulation in hepatocytes, and the additional fibrotic changes happen during NASH. We aim to investigate the role of genetic risk markers of NAFLD in liver lipid metabolism by performing liver lipidome profiling of relatively large cohort of patients (~350). Establishing clinically-relevant reference values (in moles) of the healthy liver lipidome, its changes in obese patients, and patients with NAFLD and NASH is an essential part of translational aspect of current work. This project is performed in collaboration with Dr. Jochen Hampe from the University Hospital in Dresden, Dr. Alessandra Palladini and Dr. Uenal Coskun from Paul Langerhans Institute in Dresden, Dr. Judith Wodke and Dr. Edda Klipp from Humboldt University in Berlin, Tim Rose and Dr. Josch Pauling from Technical University in Munich (Hample lab, Coskun lab, Klipp lab, Pauling lab and LiSyM.
  • We also systematically studied the plasma lipidome composition of healthy young individuals and found that the concentration of ca 50% out of 281 quantified molecules is significantly different between male and female cohorts ('Gender, Contraceptives and Individual Metabolic Predisposition Shape a Healthy Plasma Lipidome.').

Original publications

Veera Raghavan Thangapandi, Oskar Knittelfelder, Mario Brosch, Eleonora Patsenker, Olga Vvedenskaya, Stephan Buch, Sebastian Hinz, Alexander Hendricks, Marina Nati, Alexander Herrmann, Devavrat Ravindra Rekhade, Thomas Berg, Madlen Matz-Soja, Klaus Huse, Edda Klipp, Josch K Pauling, Judith Ah Wodke, Jacobo Miranda Ackerman, Malte von Bonin, Elmar Aigner, Christian Datz, Witigo von Schönfels, Sophie Nehring, Sebastian Zeissig, Christoph Röcken, Andreas Dahl, Trian Chavakis, Felix Stickel, Andrej Shevchenko, Clemens Schafmayer, Jochen Hampe, Pallavi Subramanian
Loss of hepatic Mboat7 leads to liver fibrosis.
Gut, Art. No. doi: 10.1136/gutjnl-2020-320853 (2020)  DOI 

Yuting Wang, Sebastian Hinz, Ortrud Uckermann, Pia Hönscheid, Witigo von Schönfels, Greta Burmeister, Alexander Hendricks, Jacobo Miranda Ackerman, Gustavo Baretton, Jochen Hampe, Mario Brosch, Clemens Schafmayer, Andrej Shevchenko, Sebastian Zeissig
Shotgun lipidomics-based characterization of the landscape of lipid metabolism in colorectal cancer.
Biochim Biophys Acta Mol Cell Biol Lipids, 1865(3) Art. No. 158579 (2020) DOI 

Olga Vvedenskaya, Yuting Wang, Jacobo Miranda Ackerman, Oskar Knittelfelder, Andrej Shevchenko
Analytical challenges in human plasma lipidomics: A winding path towards the truth
Trends Analyt Chem, Art. No. 115277 (2018) DOI 

Susanne Sales, Juergen Graessler, Sara Ciucci, Rania Al-Atrib, Terhi Vihervaara, Kai Schuhmann, Dimple Kauhanen, Marko Sysi-Aho, Stefan Bornstein, Marc Bickle, Carlo Vittorio Cannistraci, Kim Ekroos, Andrej Shevchenko
Gender, Contraceptives and Individual Metabolic Predisposition Shape a Healthy Plasma Lipidome.
Sci Rep, 6 Art. No. 27710 (2016) PDF   DOI 

Susanne Sales, Oskar Knittelfelder, Andrej Shevchenko
Lipidomics of Human Blood Plasma by High-Resolution Shotgun Mass Spectrometry.
Methods Mol Biol, 1619 203-212 (2017) DOI 

Santosh Phuyal, Mayes Kasem, Oskar Knittelfelder, Animesh Sharma, Davi de Miranda Fonseca, Vaineta Vebraite, Sergey Shaposhnikov, Geir Slupphaug, Vidar Skaug, Shanbeh Zienolddiny
Characterization of the proteome and lipidome profiles of human lung cells after low dose and chronic exposure to multiwalled carbon nanotubes.
Nanotoxicology, 12(2) 138-152 (2018)  DOI 

Oskar Knittelfelder, Sofia Traikov, Olga Vvedenskaya, Andrea Schuhmann, Sandra Segeletz, Anna Shevchenko, Andrej Shevchenko
Shotgun Lipidomics Combined with Laser Capture Microdissection: A Tool To Analyze Histological Zones in Cryosections of Tissues.
Anal Chem, 90(16) 9868-9878 (2018) DOI 

Collaborations

Hample lab
Coskun lab
Klipp lab
Pauling lab and LiSyM

Solimena Lab
Matz-Soja lab

Zeissig lab
Cramer lab

Dr. Olga Vvedenskaya

Contact: clinical.lipidomics(at)mpi-cbg.de

Lost in translation? Email us to clinical.lipidomics(at)mpi-cbg.de and we will be happy to answer your questions or discuss potential collaboration projects.

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