PeakStrainer is an implementation of the paper Intensity-Independent Noise Filtering in FT MS and FT MS/MS Spectra for Shotgun Lipidomics.
PeakStrainer is a tool to de-noise high resolution FT MS and FT MS/MS spectra. It does this by removing signals that poorly repeat in successively acquired scans – independently of their intensity.
Peaks in panel B are not repeated with the required rate – within the pre-defined m/z range, they were only detected in 9% or, respectively, 27% of acquired scans – they will be removed. In contrast, peaks in panels C and D were detected in each scan and will be retained. In this way, it is possible to remove > 90% peaks without losing a single bona fide fragment.
The input to the application is a *.raw files and the output at intermediate stages are *.CSV and final results are stored as *.mzXML files.
If you use the software for a publication please use DOI 10.17617/1.47 as a reference. If you would like to reference the paper please use DOI 10.1021/acs.analchem.7b00794
If you would like to share your process and or data, please feel free to provide the data in the Issues page and we will find a place for it in the wiki.
After processing *.mzXML files will be created in the same directory as the *.raw files
To find out more and get the python source code please visit git.mpi-cbg.de/labShevchenko/PeakStrainer