The ASI SCAPE Dual-Camera is currently still under construction and will be available mid-2024. It is equipped with two cameras for simultaneous dual-color imaging and motorized filter-wheels for additional flexibility. The system also features a pulsed UV laser for cutting and homogeneous widefield illumination for uncaging (405 nm) and optogenetics (445 nm).
Instrument configuration
- Primary objective: Leica HC PL APO 40x / 1.10 W CORR
- Total magnification: 67x
- Field of view: 320 µm x 320 um (with galvo) 320 x 2 mm (with stage)
- Effective NA (theoretical): ~0.7 in x, ~1.05 in y
- Measured resolution: ~450 nm in x, ~350 nm in y, ~1.1 µm in z
- Imaging camera: two Kinetix22 Scientific CMOS for simultaneous multi-channel detection
- Frame rate: up to 498 FPS, single plane
- Available laser lines: 405 nm / 445 nm / 488 nm / 532 nm / 561 nm / 642 nm
- Detection filters: Motorized filter wheels broad selection of different filters
- Software: Micromanager 1.4 / 2.0 with plugin for system control
- Type of dishes: compatible with well plates, 35 mm dishes, and chamber slides
- Environmental control: stage top incubator for temperature / CO2 control available, automated water immersion medium dispenser
- Photomanipulation: 355 nm point-scanning, 405 nm and 445 nm widefield illumination
