Pacific Biosciences (PacBio) SEQUEL and SEQUEL2

single-molecule real time (SMRT) DNA sequencing on the SEQUEL devices:

PacBio SMRT sequencing is used to sequence various long templates such as long genomic DNA, transcribed RNA and long amplified PCR fragments. It comes in two flavors: a) highly precise HiFi sequencing of medium-size DNA (10-15 kb in length, sequence accuracy > 99,9%) and b) continuous long reads of long DNA fragments (> 30 kb, up to 100 kb in length, sequence accuracy circa 88-90%).

Oxford Nanopore (ONT) PromethION

an alternative strategy for long-read and single molecule sequencing of gDNA, RNA and amplicons:

It supports sequencing of ultra-long genomic DNA (200 kb and more) or direct sequencing of RNA without the need of cDNA transcription. The recorded accuracy of ONT reads is around 90%.

Optical mapping

Bionano Saphyr

to generate optical maps of long and ultra-long genomic DNA molecules.

Optical mapping is used to improve the contig continuity while scaffolding in our de novo genome assembly pipeline. In addition, the technology allows the detection structural variants such as deletions, insertions, inversions, and translocations that are larger than 500 bp in genomes by comparing the genome of interest against a reference genome.

Automated capillary electrophoresis

for separation of DNA fragments across different scales (100 bp to more than 500 kb) at high resolution and minor input.

The Fragment analyzer is used for automated and high-throughput capillary electrophoresis of PCR-fragments (fragment sizes 100 bp – 2 kb). We also offer two pulse-field electrophoresis systems (Femtopulse and Pippin pulse) to resolve and separate long and ultra-long DNA fragments.