C. elegans genome editing by microinjection

C.elegans Knock-Out strain

  • Guide RNA design and synthesis
  • In vivo injection
  • Worm picking and screening
  • Genotyping
  • Sequencing including sequence validation
  • Freezing and Remp storage

C.elegans tagging

  • Ordering of 2x HDR templates-Guide RNA design and synthesis
  • In vivo injection
  • Worm picking and screening
  • Genotyping
  • Fluorescence microscopy
  • Sequencing including sequence validation
  • Freezing and Remp storage