Mouse genome editing by pronuclear injection in collaboration with Transgenic Core Facility
Knock-Out/Pointmutation Mouse
- Guide RNA design and synthesis
- Pronuclear Injection by TCF-Pointmutation: HDR template
- TOPO- cloning
- Sequencing including sequence validation
Knock-In Mouse
- Guide RNA design and synthesis
- Pronuclear Injection by TCF
- HDR construct cloning
- TOPO- cloning
- Sequencing including sequence validation
