Shotgun Lipidomics by Ultra-High Resolution Orbitrap Fourier Transform Mass Spectrometry

Shotgun lipidomics provides a quantitative (as molar concentrations) snapshot of the lipidome at a given time. However, the lipid flux (i.e. rates of lipid synthesis and degradation) is vital to understand the real dynamics of the biological system. Lipidome-wide flux analysis with stable isotope labeling requires high mass resolution (e.g. > 500,000) for baseline separation of isotopic peaks of unlabeled and labelled lipids. To attain ultra-high resolution, we employ an external data acquisition system in parallel to the conventional system (Q Exactive Orbitrap MS) without compromising the scope, accuracy, and dynamic range of full-lipidome quantitative shotgun profiling. We are interested in monitoring the lipid turnover rates by quantifying labeled lipids and products of their metabolic transformation during aging and neurodegenerative diseases with a combination of sUHR lipidomics workflow and metabolic 15N labeling of mouse.

Original Publication

Knittelfelder, O. et al. (2018) ‘Shotgun Lipidomics Combined with Laser Capture Microdissection: A Tool to Analyze Histological Zones in Cryosections of Tissues’, Analytical Chemistry, 90(16), pp. 9868–9878. doi: 10.1021/acs.analchem.8b02004.


Fornasiero lab
Spectroswiss Sarl
Nadler lab
Coskun lab


Scientific Computing Facility, Max Planck Institute of Molecular Cell Biology and Genetics, Dresden
Prof. Dr. Michele Solimena Group (Paul Langerhans Institute Dresden)
Prof. Dr. Jochen Hampe Group (Universitätsklinikum Carl Gustav Carus, Dresden)
Prof. Dr. Sebastian Zeissig Group (Center for Regenerative Therapies, Dresden)

Vannuruswamy Garikapati

Contact: garikapati(at)