- Alexander von Appen
- Jan Brugués
- Dye / Eaton
- Anne Grapin-Botton
- Stephan Grill
- Pierre Haas
- Alf Honigmann
- Meritxell Huch
- Wieland Huttner
- Anthony Hyman
- Elisabeth Knust
- Moritz Kreysing
- Rita Mateus
- Carl Modes
- Gene Myers
- André Nadler
- Jonathan Rodenfels
- Ivo Sbalzarini
- Andrej Shevchenko
- Jacqueline Tabler
- Dora Tang
- Pavel Tomancak
- Agnes Toth-Petroczy
- Jesse Veenvliet
- Christoph Zechner
- Marino Zerial
Tissue maintenance and repair depend on the ability of cells to respond to internal and external cues, proliferate and differentiate into functional components. Aberrant regulation of this response results in disease; hyperproliferation and cancer or tissue degeneration and scar formation. In the Huch lab we exploit both the liver, as a model of extensive regenerative capacity and the pancreas, which exhibits very little regeneration potential, to unveil the biological processes that control adult tissue homeostasis and repair and their deregulation in disease.
Our experimental strategy involves the combined use of: (1) animal models and (2) in vitro mouse and human organoid models that recapitulate key aspects of mouse and human liver development, proliferation and regeneration in culture, in a controlled environment.
We take the following approaches:
- We study the different subpopulations of liver progenitors during early liver development
- We investigate the niche-progenitor relationship during regeneration and in disease
- We analyse the epigenetic mechanisms involved in the transition from a differentiated cell to an activated progenitor and from a progenitor to a differentiated cell
- We study the cellular heterogeneity and cellular interactions in human cancer
Our long-term goal is to understand the principles that govern proliferation and differentiation of adult organs and tissues to gain the knowledge required to further develop our organoid cultures and potentially recapitulate organogenesis in vitro.
* joined first author
# joined corresponding author
Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation.
Cell Stem Cell, 28(11) 1907-1921 (2021)
Long-term expansion, genomic stability and in vivo safety of adult human pancreas organoids.
BMC Dev Biol, 20(1) Art. No. 4 (2020)
Epigenetic remodelling licences adult cholangiocytes for organoid formation and liver regeneration.
Nat Cell Biol, 21(11) 1321-1333 (2019)
Nicole Prior, Christopher J Hindley, Fabian Rost, Elena Meléndez, Winnie W Y Lau, Berthold Göttgens, Steffen Rulands, Benjamin D Simons, Meritxell Huch
Lgr5+ stem and progenitor cells reside at the apex of a heterogeneous embryonic hepatoblast pool.
Development, 146(12) Art. No. dev174557 (2019)
Human primary liver cancer-derived organoid cultures for disease modeling and drug screening.
Nat Med, 23(12) 1424-1435 (2017)
Culture and establishment of self-renewing human and mouse adult liver and pancreas 3D organoids and their genetic manipulation.
Nat Protoc, 11(9) 1724-1743 (2016)
Long-term culture of genome-stable bipotent stem cells from adult human liver.
Cell, 160(1-2) 299-312 (2015)
Unlimited in vitro expansion of adult bi-potent pancreas progenitors through the Lgr5/R-spondin axis.
EMBO J, 32(20) 2708-2721 (2013)
Meritxell Huch, Craig Dorrell, Sylvia F Boj, Johan H van Es, Vivian S W Li, Marc van de Wetering, Toshiro Sato, Karien Hamer, Nobuo Sasaki, Milton J Finegold, Annelise Haft, Robert R G Vries, Markus Grompe, Hans Clevers
In vitro expansion of single Lgr5+ liver stem cells induced by Wnt-driven regeneration.
Nature, 494(7436) 247-250 (2013)