Research Groups

* joined first author # joined corresponding author

2021

Lydie Flasse#, Coline Schewin, Anne Grapin-Botton#
Pancreas morphogenesis: Branching in and then out.
Curr. Top. Dev. Biol., 143 75-110 (2021)
DOI

2020

Allison Lewis✳︎, Rashmiparvathi Keshara✳︎, Yung Hae Kim#, Anne Grapin-Botton#
Self-organization of organoids from endoderm-derived cells.
J Mol Med (Berl), 99(4) 449-462 (2020)
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Dror Sever#, Anne Grapin-Botton#
Regeneration of the pancreas: proliferation and cellular conversion of surviving cells.
Curr Opin Genet Dev, 64 84-93 (2020)
DOI
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Keiichi Katsumoto, Anne Grapin-Botton
Nutrients men-TOR β-Cells to Adulthood.
Dev Cell, 54(2) 140-141 (2020)
DOI
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Lydie Flasse#, Siham Yennek, Cédric Cortijo, Irene Seijo Barandiaran, Marine R-C Kraus, Anne Grapin-Botton#
Apical Restriction of the Planar Cell Polarity Component VANGL in Pancreatic Ducts Is Required to Maintain Epithelial Integrity.
Cell Rep, 31(8) Art. No. 107677 (2020)
DOI

2019

Lukas Huijbregts, Maja Borup Kjær Petersen, Claire Berthault, Mattias Hansson, Virginie Aiello, Latif Rachdi, Anne Grapin-Botton, Christian Honore, Raphael Scharfmann
Bromodomain and Extra Terminal Protein Inhibitors Promote Pancreatic Endocrine Cell Fate.
Diabetes, 68(4) 761-773 (2019)
DOI

2018

Natalia Petersen, Thomas M Frimurer, Marianne Terndrup Pedersen, Kristoffer L Egerod, Nicolai J Wewer Albrechtsen, Jens J Holst, Anne Grapin-Botton, Kim B Jensen, Thue W Schwartz
Inhibiting RHOA Signaling in Mice Increases Glucose Tolerance and Numbers of Enteroendocrine and Other Secretory Cells in the Intestine.
Gastroenterology, 155(4) 1164-1176 (2018)
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Cyrille Ramond, Belin Selcen Beydag-Tasöz, Ajuna Azad, Martijn van de Bunt, Maja Borup Kjær Petersen, Nicola L Beer, Nicolas Glaser, Claire Berthault, Anna L Gloyn, Mattias Hansson, Mark I McCarthy, Christian Honoré, Anne Grapin-Botton, Raphael Scharfmann
Understanding human fetal pancreas development using subpopulation sorting, RNA sequencing and single-cell profiling.
Development, 145(16) Art. No. dev165480 (2018)
DOI
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Svend Bertel Dahl-Jensen, Siham Yennek, Lydie Flasse, Hjalte List Larsen, Dror Sever, Gopal Karremore, Ivana Novak, Kim Sneppen, Anne Grapin-Botton
Deconstructing the principles of ductal network formation in the pancreas.
PLoS Biol, 16(7) 2002842-2002842 (2018)
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Violeta Georgieva Tsonkova, Fredrik Wolfhagen Sand, Xenia Asbæk Wolf, Lars Groth Grunnet, Anna Kirstine Ringgaard, Camilla Ingvorsen, Louise Winkel, Mark Kalisz, Kevin Dalgaard, Christine Bruun, Johannes Fels, Charlotte Helgstrand, Sven Hastrup, Fredrik Kryh Öberg, Erik Vernet, Michael Paolo Bastner Sandrini, Allan Christian Shaw, Carsten Jessen, Mads Grønborg, Jacob Hald, Hanni Willenbrock, Dennis Madsen, Rasmus Wernersson, Lena Hansson, Jan Nygaard Jensen, Annette Plesner, Tomas Alanentalo, Maja Borup Kjær Petersen, Anne Grapin-Botton, Christian Honoré, Jonas Ahnfelt-Rønne, Jacob Hecksher-Sørensen, Philippe Ravassard, Ole D Madsen, Claude Rescan, Thomas Frogne
The EndoC-βH1 cell line is a valid model of human beta cells and applicable for screenings to identify novel drug target candidates.
Mol Metab, 8 144-157 (2018)
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Maja Borup Kjær Petersen, Carla A C Gonçalves, Yung Hae Kim, Anne Grapin-Botton
Recapitulating and Deciphering Human Pancreas Development From Human Pluripotent Stem Cells in a Dish.
Curr. Top. Dev. Biol., 129 143-190 (2018)
DOI

2017

Maja Borup Kjær Petersen, Ajuna Azad, Camilla Ingvorsen, Katja Hess, Mattias Hansson, Anne Grapin-Botton, Christian Honoré
Single-Cell Gene Expression Analysis of a Human ESC Model of Pancreatic Endocrine Development Reveals Different Paths to β-Cell Differentiation.
Stem Cell Reports, 9(4) 1246-1261 (2017)
DOI
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Hjalte List Larsen, Laura Martín-Coll, Alexander Valentin Nielsen, Christopher V E Wright, Ala Trusina, Yung Hae Kim, Anne Grapin-Botton
Stochastic priming and spatial cues orchestrate heterogeneous clonal contribution to mouse pancreas organogenesis.
Nat Commun, 8(1) Art. No. 605 (2017)
DOI
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Hjalte List Larsen, Anne Grapin-Botton
The molecular and morphogenetic basis of pancreas organogenesis.
Semin Cell Dev Biol, 66 51-68 (2017)
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Svend Bertel Dahl-Jensen, Anne Grapin-Botton
The physics of organoids: a biophysical approach to understanding organogenesis.
Development, 144(6) 946-951 (2017)
DOI
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Anne Grapin-Botton, Palle Serup
Parsing the Pancreas.
N. Engl. J. Med., 376(9) 886-888 (2017)
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David Martin, Anne Grapin-Botton
The Importance of REST for Development and Function of Beta Cells.
Front Cell Dev Biol, 5 12-12 (2017)
DOI

2016

Corinne Berclaz, Daniel Szlag, David Nguyen, Jérôme Extermann, Arno Bouwens, Paul J Marchand, Julia Nilsson, Anja Schmidt-Christensen, Dan Holmberg, Anne Grapin-Botton, Theo Lasser
Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow.
Biomed Opt Express, 7(11) 4569-4580 (2016)
DOI
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Anne Grapin-Botton
Three-dimensional pancreas organogenesis models.
Diabetes Obes Metab, 18 Suppl 1 33-40 (2016)
DOI
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Corinne Berclaz, Anja Schmidt-Christensen, Daniel Szlag, Jérôme Extermann, Lisbeth Hansen, Arno Bouwens, Martin Villiger, Joan Goulley, Frans Schuit, Anne Grapin-Botton, Theo Lasser, Dan Holmberg
Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging.
Diabetologia, 59(3) 550-559 (2016)
DOI
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Svend Bertel Dahl-Jensen, Manuel Figueiredo-Larsen, Anne Grapin-Botton, Kim Sneppen
Short-range growth inhibitory signals from the epithelium can drive non-stereotypic branching in the pancreas.
Physical biology, 13(1) 16007-16007 (2016)
DOI

2015

David Martin, Yung-Hae Kim, Dror Sever, Chai-An Mao, Jacques-Antoine Haefliger, Anne Grapin-Botton
REST represses a subset of the pancreatic endocrine differentiation program.
Dev Biol, 405(2) 316-327 (2015)
DOI
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Elke A Ober, Anne Grapin-Botton
At new heights - endodermal lineages in development and disease.
Development, 142(11) 1912-1917 (2015)
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Corinne Berclaz, Christophe Pache, Arno Bouwens, Daniel Szlag, Antonio Lopez, Leo A B Joosten, Selen Ekim, Maarten Brom, Martin Gotthardt, Anne Grapin-Botton, Theo Lasser
Combined Optical Coherence and Fluorescence Microscopy to assess dynamics and specificity of pancreatic beta-cell tracers.
Sci Rep, 5 10385-10385 (2015)
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Anne Grapin-Botton, Philip Allan Seymour, Gérard Gradwohl
Pairing-up SOX to kick-start beta cell genesis.
Diabetologia, 58(5) 859-861 (2015)
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Yung Hae Kim, Hjalte List Larsen, Pau Rué, Laurence A Lemaire, Jorge Ferrer, Anne Grapin-Botton
Cell cycle-dependent differentiation dynamics balances growth and endocrine differentiation in the pancreas.
PLoS Biol, 13(3) Art. No. e1002111 (2015)
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Laurence A Lemaire, Joan Goulley, Yung Hae Kim, Solenne Carat, Patrick Jacquemin, Jacques Rougemont, Daniel B Constam, Anne Grapin-Botton
Bicaudal C1 promotes pancreatic NEUROG3+ endocrine progenitor differentiation and ductal morphogenesis.
Development, 142(5) 858-870 (2015)
DOI
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Chiara Greggio, Filippo De Franceschi, Anne Grapin-Botton
Concise reviews: In vitro-produced pancreas organogenesis models in three dimensions: self-organization from few stem cells or progenitors.
Stem Cells, 33(1) 8-14 (2015)
DOI

2014

Spencer G Willet, Michael A Hale, Anne Grapin-Botton, Mark A Magnuson, Raymond J MacDonald, Christopher V E Wright
Dominant and context-specific control of endodermal organ allocation by Ptf1a.
Development, 141(22) 4385-4394 (2014)
DOI
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Chiara Greggio, Filippo De Franceschi, Manuel Figueiredo-Larsen, Anne Grapin-Botton
In vitro pancreas organogenesis from dispersed mouse embryonic progenitors.
J Vis Exp, (89) 1-1 (2014)
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Julie Hanotel, Nathalie Bessodes, Aurore Thélie, Marie Hedderich, Karine Parain, Benoit Van Driessche, Karina De Oliveira Brandão, Sadia Kricha, Mette C Jorgensen, Anne Grapin-Botton, Palle Serup, Carine Van Lint, Muriel Perron, Tomas Pieler, Kristine A Henningfeld, Eric J Bellefroid
The Prdm13 histone methyltransferase encoding gene is a Ptf1a-Rbpj downstream target that suppresses glutamatergic and promotes GABAergic neuronal fate in the dorsal neural tube.
Dev Biol, 386(2) 340-357 (2014)
DOI
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P. Rué, Y.H. Kim, H. List Larsen, A. Grapin-Botton, A. Martinez Arias
A framework for the analysis of symmetric and asymmetric divisions in developmental processes.
bioRxiv, Art. No. https://doi.org/10.1101/010835 (2014)
DOI

2013

Chiara Greggio, Filippo De Franceschi, Manuel Figueiredo-Larsen, Samy Gobaa, Adrian Ranga, Henrik Semb, Matthias Lutolf, Anne Grapin-Botton
Artificial three-dimensional niches deconstruct pancreas development in vitro.
Development, 140(21) 4452-4462 (2013)
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Apriliana E R Kartikasari, Josie X Zhou, Murtaza S Kanji, David N Chan, Arjun Sinha, Anne Grapin-Botton, Mark A Magnuson, William E Lowry, Anil Bhushan
The histone demethylase Jmjd3 sequentially associates with the transcription factors Tbx3 and Eomes to drive endoderm differentiation.
EMBO J, 32(10) 1393-1408 (2013)
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Xiaoling Qu, Solomon Afelik, Jan Nygaard Jensen, Michael A Bukys, Sune Kobberup, Martin Schmerr, Fan Xiao, Pia Nyeng, Maria Veronica Albertoni, Anne Grapin-Botton, Jan Nygaard Jensen
Notch-mediated post-translational control of Ngn3 protein stability regulates pancreatic patterning and cell fate commitment.
Dev Biol, 376(1) 1-12 (2013)
DOI

2012

Cédric Cortijo, Mathieu Gouzi, Fadel Tissir, Anne Grapin-Botton
Planar cell polarity controls pancreatic beta cell differentiation and glucose homeostasis.
Cell Rep, 2(6) 1593-1606 (2012)
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Palle Serup, Carsten Gustavsen, Tino Klein, Leah A Potter, Robert Lin, Nandita Mullapudi, Ewa Wandzioch, Angela Hines, Ashley Davis, Christine Bruun, Nina Engberg, Dorthe R Petersen, Janny M L Peterslund, Raymond J Macdonald, Anne Grapin-Botton, Mark A Magnuson, Kenneth S Zaret
Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice.
Dis Model Mech, 5(6) 956-966 (2012)
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Eunyoung Choi, Marine R-C Kraus, Laurence A Lemaire, Momoko Yoshimoto, Sasidhar Vemula, Leah A Potter, Elisabetta Manduchi, Christian J Stoeckert, Anne Grapin-Botton, Mark A Magnuson
Dual lineage-specific expression of Sox17 during mouse embryogenesis.
Stem Cells, 30(10) 2297-2308 (2012)
DOI
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Marine R-C Kraus, Anne Grapin-Botton
Patterning and shaping the endoderm in vivo and in culture.
Curr Opin Genet Dev, 22(4) 347-353 (2012)
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Hung Ping Shih, Janel L Kopp, Manbir Sandhu, Claire L Dubois, Philip Allan Seymour, Anne Grapin-Botton, Maike Sander
A Notch-dependent molecular circuitry initiates pancreatic endocrine and ductal cell differentiation.
Development, 139(14) 2488-2499 (2012)
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Corinne Berclaz, Joan Goulley, Martin Villiger, Christophe Pache, Arno Bouwens, Erica Martin-Williams, Dimitri Van de Ville, Anthony C Davison, Anne Grapin-Botton, Theo Lasser
Diabetes imaging-quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy.
Biomed Opt Express, 3(6) 1365-1380 (2012)
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Nancy Thompson, Emilie Gésina, Peter Scheinert, Philipp Bucher, Anne Grapin-Botton
RNA profiling and chromatin immunoprecipitation-sequencing reveal that PTF1a stabilizes pancreas progenitor identity via the control of MNX1/HLXB9 and a network of other transcription factors.
Mol Cell Biol, 32(6) 1189-1199 (2012)
DOI
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Marine R-C Kraus, Séverine Clauin, Yvan Pfister, Massimo Di Maïo, Tim Ulinski, Daniel B Constam, Christine Bellanné-Chantelot, Anne Grapin-Botton
Two mutations in human BICC1 resulting in Wnt pathway hyperactivity associated with cystic renal dysplasia.
Hum Mutat, 33(1) 86-90 (2012)
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Marie Boutant, Oscar Henrique Pereira Ramos, Cécile Tourrel-Cuzin, Jamileh Movassat, Anissa Ilias, David Vallois, Julien Planchais, Jean-Paul Pégorier, Frans Schuit, Patrice X Petit, Pascale Bossard, Kathrin Maedler, Anne Grapin-Botton, Mireille Vasseur-Cognet
COUP-TFII controls mouse pancreatic β-cell mass through GLP-1-β-catenin signaling pathways.
PLoS ONE, 7(1) 30847-30847 (2012)
DOI

2011

Mathieu Gouzi, Yung Hae Kim, Keiichi Katsumoto, Kerstin A Johansson, Anne Grapin-Botton
Neurogenin3 initiates stepwise delamination of differentiating endocrine cells during pancreas development.
Dev Dyn, 240(3) 589-604 (2011)
DOI

2010

Martin Villiger, Joan Goulley, Erica Martin-Williams, Anne Grapin-Botton, Theo Lasser
Towards high resolution optical imaging of beta cells in vivo.
Curr. Pharm. Des., 16(14) 1595-1608 (2010)
DOI

2009

Martin Villiger, Joan Goulley, M Friedrich, Anne Grapin-Botton, Paolo Meda, Theo Lasser, R A Leitgeb
In vivo imaging of murine endocrine islets of Langerhans with extended-focus optical coherence microscopy.
Diabetologia, 52(8) 1599-1607 (2009)
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Elke Bayha, Mette C Jørgensen, Palle Serup, Anne Grapin-Botton
Retinoic acid signaling organizes endodermal organ specification along the entire antero-posterior axis.
PLoS ONE, 4(6) 5845-5845 (2009)
DOI

2008

Anne Grapin-Botton
Endoderm specification.
In: StemBook [Internet].. DOI: 10.3824/stembook.1.30.1 .,Cambridge (MA),Harvard Stem Cell Institute (2008),1-1
DOI

2007

Anne Grapin-Botton, Harry Heimberg, Frédéric Lemaigre
The genetic programme of pancreatic beta-cells: basic science for the development of beta-cell therapy. Workshop on programming pancreatic beta-cells.
EMBO Rep, 8(4) 322-326 (2007)
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Anne Grapin-Botton, Daniel B Constam
Evolution of the mechanisms and molecular control of endoderm formation.
Mech Dev, 124(4) 253-278 (2007)
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Kerstin A Johansson, Umut Dursun, Nathalie Jordan, Guoqiang Gu, Friedrich Beermann, Gérard Gradwohl, Anne Grapin-Botton
Temporal control of neurogenin3 activity in pancreas progenitors reveals competence windows for the generation of different endocrine cell types.
Dev Cell, 12(3) 457-465 (2007)
DOI

2006

Jessica Dessimoz, Anne Grapin-Botton
Pancreas development and cancer: Wnt/beta-catenin at issue...
Cell Cycle, 5(1) 7-10 (2006)
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Jessica Dessimoz, Robert Opoka, Jennifer J Kordich, Anne Grapin-Botton, James M Wells
FGF signaling is necessary for establishing gut tube domains along the anterior-posterior axis in vivo.
Mech Dev, 123(1) 42-55 (2006)
DOI

2005

Jessica Dessimoz, Claude Bonnard, Joerg Huelsken, Anne Grapin-Botton
Pancreas-specific deletion of beta-catenin reveals Wnt-dependent and Wnt-independent functions during development.
Curr Biol, 15(18) 1677-1683 (2005)
DOI
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Anne Grapin-Botton
Ductal cells of the pancreas.
Int. J. Biochem. Cell Biol., 37(3) 504-510 (2005)
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Anne Grapin-Botton
Antero-posterior patterning of the vertebrate digestive tract: 40 years after Nicole Le Douarin's PhD thesis.
Int. J. Dev. Biol., 49(2-3) 335-347 (2005)
DOI

2004

Anne Grapin-Botton, Daniel B Constam
Endoderm development.
In: Gastrulation : from cells to embryo.,Cold Spring Harbor, N.Y,Cold Spring Harbor Laboratory Press (2004),433-448 Ch. 30

2003

Maya Kumar, Nathalie Jordan, Douglas A Melton, Anne Grapin-Botton
Signals from lateral plate mesoderm instruct endoderm toward a pancreatic fate.
Dev Biol, 259(1) 109-122 (2003)
DOI

2002

K A Johansson, Anne Grapin-Botton
Development and diseases of the pancreas.
Clin. Genet., 62(1) 14-23 (2002)
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Anne Grapin-Botton
Les étapes du développement du pancréas: des pistes pour le traitement du diabète.
Med Sci (Paris), 18 467-473 (2002)

2001

Anne Grapin-Botton, A R Majithia, Douglas A Melton
Key events of pancreas formation are triggered in gut endoderm by ectopic expression of pancreatic regulatory genes.
Genes Dev, 15(4) 444-454 (2001)
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K Ladjali-Mohammedi, Anne Grapin-Botton, M A Bonnin, N M Le Douarin
Distribution of HOX genes in the chicken genome reveals a new segment of conservation between human and chicken.
Cytogenet. Cell Genet., 92(1-2) 157-161 (2001)
DOI

2000

Anne Grapin-Botton, Douglas A Melton
Endoderm development: from patterning to organogenesis.
Trends Genet., 16(3) 124-130 (2000)
DOI

1999

Anne Grapin-Botton, F Cambronero, H L Weiner, M A Bonnin, L Puelles, N M Le Douarin
Patterning signals acting in the spinal cord override the organizing activity of the isthmus.
Mech Dev, 84(1-2) 41-53 (1999)
DOI

1998

G Couly, Anne Grapin-Botton, P Coltey, B Ruhin, N M Le Douarin
Determination of the identity of the derivatives of the cephalic neural crest: incompatibility between Hox gene expression and lower jaw development.
Development, 125(17) 3445-3459 (1998)
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V Nataf, Anne Grapin-Botton, D Champeval, A Amemiya, M Yanagisawa, N M Le Douarin
The expression patterns of endothelin-A receptor and endothelin 1 in the avian embryo.
Mech Dev, 75(1-2) 145-149 (1998)
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A M Mager, Anne Grapin-Botton, K Ladjali, D Meyer, C M Wolff, P Stiegler, M A Bonnin, P Remy
The avian fli gene is specifically expressed during embryogenesis in a subset of neural crest cells giving rise to mesenchyme.
Int. J. Dev. Biol., 42(4) 561-572 (1998)
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Anne Grapin-Botton, M A Bonnin, M Sieweke, N M Le Douarin
Defined concentrations of a posteriorizing signal are critical for MafB/Kreisler segmental expression in the hindbrain.
Development, 125(7) 1173-1181 (1998)

1997

A Eichmann, Anne Grapin-Botton, L Kelly, T Graf, N M Le Douarin, M Sieweke
The expression pattern of the mafB/kr gene in birds and mice reveals that the kreisler phenotype does not represent a null mutant.
Mech Dev, 65(1-2) 111-122 (1997)
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Anne Grapin-Botton, M A Bonnin, N M Le Douarin
Hox gene induction in the neural tube depends on three parameters: competence, signal supply and paralogue group.
Development, 124(4) 849-859 (1997)
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N M Le Douarin, A Grapin-Botton
Contrôle génétique du développement du rhombencéphale par les gènes Hox étudié chez l'embryon d'oiseau par la méthode des chimères caille-poulet [Genetic control of rhombencephalon development by Hox genes studied in bird embryo by the quail-chick chimera method].
C R Seances Soc Biol Fil, 191(1) 29-42 (1997)

1996

G Couly, Anne Grapin-Botton, P Coltey, N M Le Douarin
The regeneration of the cephalic neural crest, a problem revisited: the regenerating cells originate from the contralateral or from the anterior and posterior neural fold.
Development, 122(11) 3393-3407 (1996)
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Nicole M. Le Douarin, Anne Grapin-Botton, Martin Catala
Patterning of the neural primordium in the avian embryo.
Semin Cell Dev Biol, 7(2) 157-167 (1996)

1995

Anne Grapin-Botton, M A Bonnin, L A McNaughton, Robb Krumlauf, N M Le Douarin
Plasticity of transposed rhombomeres: Hox gene induction is correlated with phenotypic modifications.
Development, 121(9) 2707-2721 (1995)