Alvéole Primo Micropatterning System

The micropatterning system is a Nikon Eclipse Ti2 fluorescence  widefield microscope equipped with an Alveolé Primo-1 digital micromirror device (DMD) that allows illumination of the field of view with a pattern. By illuminating the sample with UV light, the system enables creating patterns of surface coating on glass substrates and TEM grids for electron microscopy (e.g. local PEG passivation). In addition, the system is capable to generate 3D hydrogel structures on surfaces.

Instrument configuration

  • Primary objective: Nikon 20x/04.5 objective
  • Illumination laser: 375 nm
  • DMD-size: 1824 pixel x 1140 pixel
  • Illuminated field: approx. 500 um x 300 um (20x objective)
  • Pixel size: approx. 0.29 um (20x objective)
  • Effective resolution of patterning: 1 - 2 um
  • Possible heights of hydrogel structures: 40 - 600 um (depending on gel, photoinitiator, and dose of illuminated UV light)
  • Speed: approx. 0.5 s per field of view, depending on dose
  • Software: Leonardo control software

Location