- Alexander von Appen
- Jan Brugués
- Dye / Eaton
- Anne Grapin-Botton
- Stephan Grill
- Pierre Haas
- Alf Honigmann
- Meritxell Huch
- Wieland Huttner
- Anthony Hyman
- Florian Jug
- Elisabeth Knust
- Moritz Kreysing
- Rita Mateus
- Carl Modes
- Gene Myers
- André Nadler
- Gaia Pigino
- Jonathan Rodenfels
- Ivo Sbalzarini
- Andrej Shevchenko
- Jacqueline Tabler
- Dora Tang
- Pavel Tomancak
- Agnes Toth-Petroczy
- Jesse Veenvliet
- Christoph Zechner
- Marino Zerial
General Contact Information
Max Planck Institute
of Molecular Cell Biology and Genetics
- Grapin-Botton -
|Phone||+49 351 210-2730|
|Fax||+49 351 210-1409|
Current Lab Members
|Beydag-Tasöz, Belin Selcen||Predocemail@example.com||+49 351 210-2677|
|D'Costa, Joyson Verner||Guestfirstname.lastname@example.org|
|Dietze, Sophia||Apprentice Biologielaborantemail@example.com|
|Franz, Antje||Assistant to Anne Grapin-Bottonfirstname.lastname@example.org||+49 351 210-2730|
|Grapin-Botton, Anne||Directoremail@example.com||+49 351 210-2500|
|Katsumoto, Keiichi||Staff Scientistfirstname.lastname@example.org||+49 351 210-2672|
|Keshara, Rashmiparvathi||Predocemail@example.com||+49 351 210-2605|
|Kim, Yung Hae||Staff Scientistfirstname.lastname@example.org||+49 351 210-2671|
|Lee, Byung Ho||Postdocemail@example.com||+49 351 210-2983|
|Leuschner, Marit||Technicianfirstname.lastname@example.org||+49 351 210-2736|
|Lewis, Allison||Predocemail@example.com||+49 351 210-2857|
|Petzold, Heike||Technicianfirstname.lastname@example.org||+49 351 210-2743|
|Schewin, Coline||Predocemail@example.com||+49 351 210-2676|
|Seijo Barandiaran, Irene||Postdocfirstname.lastname@example.org||+49 351 210-2984|
|Tan, Tzer Han||ELBE-Postdocemail@example.com||+49 351 210-2901|
Although there is a considerable body of work on pancreatic endocrine cells due to their importance in regulation of blood glucose levels and consequently their link to wide-spread diseases such as diabetes mellitus, the development and fate choices these cells undergo in human have not been understood in detail yet. My project focuses on characterizing human pancreatic endocrine progenitors better, and trying to understand how they choose their subsequent endocrine fates. I mainly use in vitro pancreatic differentiation systems to characterize these cells via live imaging, analyzing their endocrine subtype commitment, transcriptional profile and thus the heterogeneity within the population.
I am investigating how the planar polarity pathway can organize pancreatic progenitors in the complex network of tubes composing the pancreas. A better understanding of pancreatic epithelium architecture and how it influences cell fate choices will provide new insights into disorders associated with cyst formation and diabetes.
I study β-cell proliferation and β-cell maturation mechanisms. Diabetes is caused by many factors, but basically, diabetes patients have a problem in producing Insulin to control blood glucose level, which can involve decreased β-cell number and impaired β-cell function. If we can control β-cell proliferation and β-cell maturation in their body, we hope to overcome diabetes. I more specifically study Wnt signaling in these steps.
I’m investigating self-organisation and cell fate decisions during pancreas development using hES cell-derived pancreatic organoids. I’m using two approaches; naturally occurring mutations causing permanent neonatal diabetes and biochemical screening.
Yung Hae Kim
I'm working on differentiation dynamics of pancreatic progenitors to endocrine lineage during pancreas development by observing pancreatic progenitors' behaviors at single-cell resolution from mouse embryonic pancreas and human pluripotent stem cell (hPSC)-derived pancreatic cells. I use 2-D and 3-D live imaging, lineage tracing, single-cell transcription analysis, hPSC differentiation, and hPSC-derived pancreatic organoids. I'm also collaborating with mathematicians/physicists to model our data and test the model experimentally. The ultimate goal is to be able to control progenitor expansion and/or endocrine differentiation to boost production of beta-cells in vitro as a mean of beta-cell replacement therapy for type 1 diabetes.
Byung Ho Lee
Byung Ho is studying the influence of biophysical stimuli on the growing pancreas and pancreatic organoids. He is particularly interested in how mechanical deformations influence branching and differentiation of the pancreas and pancreatic organoids.
In Spring 2018 I was responsible primarily for the relocation of Anne’s research laboratory from Copenhagen and establishment of a functioning lab in Dresden.
I am a very enthusiastic Senior Research Technician who has worked in a number of remarkable research laboratories. I have gained extensive experience and useful working skills throughout my career in a variety of research projects.
Today I use this knowledge as I support ongoing experiments and establish new methodologies within our group, advise incoming lab members and keep the lab business running smoothly.
My research project focuses on the role of apicobasal polarity in 3D pancreatic organoid cultures. Specifically, we are currently investigating what controls cell polarity in 3D organoids compared to sphere formation.
Our state-of-the-art microscopes will help to answer these and other open questions in the future.
I work with human pluripotent stem cells, differentiate them into pancreatic cells and make pancreatic organoids. I characterize cells by flow cytometry, qPCR and immunofluorescent staining. I also take care of lab organization.
Progenitor cells are important during development to ensure there are sufficient cells to generate an organ of appropriate size and cell diversity; therefore, impairment of progenitor production, maintenance, or differentiation can result in organs of decreased size and functionality. Progenitors remain important during adulthood to ensure that as cells die they can be replenished by a cell of the correct identity. I am interested in understanding how cellular gene expression interacts with the tissue niche to control progenitor maintenance and differentiation during pancreas development, and use human organoid models to investigate this question.
It was recently shown in our lab that during development, the early secretion of fluid in the pancreas likely shapes the structure of the ductal network that deliver enzymes digesting food from their production site, in acini, to the intestine (Dahl-Jensen et al., 2018). In my project I study the influence of exocrine secretion flow on the morphogenesis of this ductal network. I am particularly interested in how the flow is generated, how it is sensed and its influence on the remodeling of the ducts.
I am studying planar cell polarity during pancreas development. I am particularly interested in understanding how tissue asymmetry is established and to analyze its role on cell differentiation.
Both, pancreas and nervous system contain highly specified cell types with shared secretory activities but distinct physiological functions i.e. metabolism vs. signal transduction. Intriguingly, many genes are shared across the two tissues. During my project, I intend to molecularly decode the conservation of gene regulatory networks (GRNs) of transcription factors, crucial for pancreas and spinal cord development. In addition, I aim to dissect the conservation of gene regulatory regions for key genes, controlling spatio-temporal gene expression. This will provide a regulatory map to help understand disease-causing mutations in non-coding regions controlling appropriate cell biogenesis in development.
Tzer Han Tan
I am interested in understanding and controlling the morphogenesis of complex 3D tissues such as pancreatic organoids. By combining novel bioengineering tools, quantitative microscopy and biophysical modelling, I hope to uncover the assembly rules underlying the emergence of functional tissue architectures.