Technology Development Groups

Publications

* joined first author # joined corresponding author

2021

Andreas Lackner✳︎, Robert Sehlke✳︎, Marius Garmhausen✳︎, Giuliano Giuseppe Stirparo✳︎, Michelle Huth, Fabian Titz-Teixeira, Petra van der Lelij, Julia Ramesmayer, Henry F Thomas, Meryem Ralser, Laura Santini, Elena Galimberti, Mihail Sarov, A F Stewart, Austin Smith, Andreas Beyer, Martin Leeb
Cooperative genetic networks drive embryonic stem cell transition from naïve to formative pluripotency.
EMBO J, Art. No. e105776 (2021)
DOI

2020

Juliana G. Roscito, Kaushikaram Subramanian, Ronald Naumann, Mihail Sarov, Anna Shevchenko, Aliona Bogdanova, Thomas Kurth, Leo Foerster, Moritz Kreysing, Michael Hiller
Recapitulating evolutionary divergence in a single cis-regulatory element is sufficient to cause expression changes of the lens gene Tdrd7.
Mol Biol Evol, 38(2) 380-392 (2020)
Open Access   DOI
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Robert W Fernandez, Kimberly Wei, Erin Y Wang, Deimante Mikalauskaite, Andrew Olson, Judy Pepper, Nakeirah Christie, Seongseop Kim, Susanne Weissenborn, Mihail Sarov, Michael R Koelle
Cellular Expression and Functional Roles of All 26 Neurotransmitter GPCRs in the C. elegans Egg-Laying Circuit.
J Neurosci, 40(39) 7475-7488 (2020)
DOI
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Alexandra Lewis, Ahmet C Berkyurek, Andre Greiner, Ahilya N Sawh, Ajay A Vashisht, Stephanie Merrett, Mathieu N Flamand, James Wohlschlegel, Mihail Sarov, Eric A Miska, Thomas F Duchaine
A Family of Argonaute-Interacting Proteins Gates Nuclear RNAi.
Mol Cell, 78(5) 862-875 (2020)
DOI
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Sören Reinke, Mary Linge, Hans H Diebner, H Luksch, Silke Glage, Anne Gocht, Avril A B Robertson, Matthew A Cooper, Sigrun R Hofmann, Ronald Naumann, Mihail Sarov, Rayk Behrendt, Axel Roers, Frank Pessler, Joachim Roesler, Angela Rösen-Wolff, Stefan Winkler
Non-canonical Caspase-1 Signaling Drives RIP2-Dependent and TNF-α-Mediated Inflammation In Vivo.
Cell Rep, 30(8) 2501-2511 (2020)
Open Access   DOI

2019

Aleksandra Spiegel, Mandy Bachmann, Gabriel Jurado Jiménez, Mihail Sarov
CRISPR/Cas9-based knockout pipeline for reverse genetics in mammalian cell culture.
Methods, 164-165 49-58 (2019)
DOI

2018

Samir Vaid, J Gray Camp, Lena Hersemann, Christina Eugster Oegema, Anne-Kristin Heninger, Sylke Winkler, Holger Brandl, Mihail Sarov, Barbara Treutlein, Wieland Huttner#, Takashi Namba#
A novel population of Hopx-dependent basal radial glial cells in the developing mouse neocortex.
Development, 145(20) Art. No. dev169276 (2018)
DOI
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Yusuke Toyoda#, Busra Akarlar, Mihail Sarov, Nurhan Ozlu, Shigeaki Saitoh#
Extracellular glucose level regulates dependence on GRP78 for cell surface localization of multipass transmembrane proteins in HeLa cells.
FEBS Lett, 592(19) 3295-3304 (2018)
DOI
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Jan-Philip Medelnik, Kathleen Roensch, Satoshi Okawa, Antonio Del Sol, Osvaldo Chara, Levan Mchedlishvili, Elly M. Tanaka
Signaling-Dependent Control of Apical Membrane Size and Self-Renewal in Rosette-Stage Human Neuroepithelial Stem Cells.
Stem Cell Reports, 10(6) 1751-1765 (2018)
Open Access   DOI
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Radoslav Aleksandrov, Anton Dotchev, Ina Poser, Dragomir Krastev, Georgi Georgiev, Greta C Panova, Yordan Babukov, Georgi Danovski, Teodora Dyankova, Lars Hubatsch, Aneliya Ivanova, Aleksandar Atemin, Marina N Nedelcheva-Veleva, Susanne Hasse, Mihail Sarov, Frank Buchholz, Anthony Hyman, Stephan W. Grill, Stoyno Stoynov
Protein Dynamics in Complex DNA Lesions.
Mol Cell, 69(6) 1046-1061 (2018)
DOI

2017

Edlyn Wu, Ajay A Vashisht, Clément Chapat, Mathieu N Flamand, Emiliano Cohen, Mihail Sarov, Yuval Tabach, Nahum Sonenberg, James Wohlschlegel, Thomas F Duchaine
A continuum of mRNP complexes in embryonic microRNA-mediated silencing.
Nucleic Acids Res, 45(4) 2081-2098 (2017)
Open Access   PDF    DOI

2016

Susanne Hasse, Anthony Hyman, Mihail Sarov
TransgeneOmics - A transgenic platform for protein localization based function exploration.
Methods, 96 69-74 (2016)
Open Access   PDF    DOI
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Sadhna Phanse, Cuihong Wan, Blake Borgeson, Fan Tu, Kevin Drew, Greg Clark, Xuejian Xiong, Olga Kagan, Julian Kwan, Alexandr Bezginov, Kyle Chessman, Swati Pal, Graham Cromar, Ophelia Papoulas, Zuyao Ni, Daniel R Boutz, Snejana Stoilova, Pierre C Havugimana, Xinghua Guo, Ramy H Malty, Mihail Sarov, Jack Greenblatt, Mohan Babu, W Brent Derry, Elisabeth R Tillier, John Wallingford, John Parkinson, Edward M Marcotte, Andrew Emili
Proteome-wide dataset supporting the study of ancient metazoan macromolecular complexes.
Data Brief, 6 715-721 (2016)
Open Access   PDF    DOI
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Nereo Kalebic, Elena Taverna, Stefania Tavano, Fong Kuan Wong, Dana Suchold, Sylke Winkler, Wieland B. Huttner, Mihail Sarov
CRISPR/Cas9-induced disruption of gene expression in mouse embryonic brain and single neural stem cells in vivo.
EMBO Rep, 17(3) 338-348 (2016)
Open Access   PDF    DOI
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Mihail Sarov#, Christiane Barz, Helena Jambor, Marco Y Hein, Christopher Schmied, Dana Suchold, Bettina Stender, Stephan Janosch, Vinay Vikas Kj, R T Krishnan, Aishwarya Krishnamoorthy, Irene R S Ferreira, Radoslaw K Ejsmont, Katja Finkl, Susanne Hasse, Philipp Kämpfer, Nicole Plewka, Elisabeth Vinis, Siegfried Schloissnig, Elisabeth Knust, Volker Hartenstein, Matthias Mann, Mani Ramaswami, K VijayRaghavan, Pavel Tomancak#, Frank Schnorrer#
A genome-wide resource for the analysis of protein localisation in Drosophila.
Elife, 5 Art. No. e12068 (2016)
Open Access   PDF    DOI

2015

Cuihong Wan, Blake Borgeson, Sadhna Phanse, Fan Tu, Kevin Drew, Greg Clark, Xuejian Xiong, Olga Kagan, Julian Kwan, Alexandr Bezginov, Kyle Chessman, Swati Pal, Graham Cromar, Ophelia Papoulas, Zuyao Ni, Daniel R Boutz, Snejana Stoilova, Pierre C Havugimana, Xinghua Guo, Ramy H Malty, Mihail Sarov, Jack Greenblatt, Mohan Babu, W Brent Derry, Elisabeth R Tillier, John Wallingford, John Parkinson, Edward M Marcotte, Andrew Emili
Panorama of ancient metazoan macromolecular complexes.
Nature, 525(7569) 339-344 (2015)
DOI
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Eric Cornes, Montserrat Porta-De-La-Riva, David Aristizábal-Corrales, Ana María Brokate-Llanos, Francisco Javier García-Rodríguez, Iris Ertl, Mònica Díaz, Laura Fontrodona, Kadri Reis, Robert Johnsen, David Baillie, Manuel J Muñoz, Mihail Sarov, Denis Dupuy, Julián Cerón
Cytoplasmic LSM-1 protein regulates stress responses through the insulin/IGF-1 signaling pathway in Caenorhabditis elegans.
RNA, 21(9) 1544-1553 (2015)
DOI
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Maria L Spletter, Christiane Barz, Assa Yeroslaviz, Cornelia Schönbauer, Irene R S Ferreira, Mihail Sarov, Daniel Gerlach, Alexander Stark, Bianca Habermann, Frank Schnorrer
The RNA-binding protein Arrest (Bruno) regulates alternative splicing to enable myofibril maturation in Drosophila flight muscle.
EMBO Rep, 16(2) 178-191 (2015)
DOI
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Sider Penkov, Damla Kaptan, Cihan Erkut, Mihail Sarov, Fanny Mende, Teymuras V. Kurzchalia
Integration of carbohydrate metabolism and redox state controls dauer larva formation in Caenorhabditis elegans.
Nat Commun, 6 Art. No. 8060 (2015)
Open Access   DOI

2014

Christian Frøkjær-Jensen, M Wayne Davis, Mihail Sarov, Jon Taylor, Stephane Flibotte, Matthew LaBella, Andrei I. Pozniakovsky, Donald G Moerman, Erik M Jorgensen
Random and targeted transgene insertion in Caenorhabditis elegans using a modified Mos1 transposon.
Nat Methods, 11(5) 529-534 (2014)
DOI
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Stephan Preibisch#, Fernando Amat, Evangelia Stamataki, Mihail Sarov, Robert H. Singer, Gene Myers, Pavel Tomancak#
Efficient Bayesian-based multiview deconvolution.
Nat Methods, 11(6) 645-648 (2014)
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Indulekha P Sudhakaran, Jens Hillebrand, Adrian Dervan, Shradha Das, Eimear E Holohan, Jörn Hülsmeier, Mihail Sarov, Roy Parker, K VijayRaghavan, Mani Ramaswami
FMRP and Ataxin-2 function together in long-term olfactory habituation and neuronal translational control.
Proc Natl Acad Sci U.S.A., 111(1) 99-108 (2014)
DOI

2013

Anna Ivanova, Yannis Kalaidzidis, Ronald Dirkx, Mihail Sarov, Michael Gerlach, Britta Schroth-Diez, Andreas Müller, Yanmei Liu, Cordula Andree, Bernard Mulligan, Carla Münster, Thomas Kurth, Marc Bickle, Stephan Speier, Konstantinos Anastassiadis, Michele Solimena
Age-dependent labeling and imaging of insulin secretory granules.
Diabetes, 62(11) 3687-3696 (2013)
DOI
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Marina N Nedelcheva-Veleva, Mihail Sarov, Ivan Yanakiev, Eva Mihailovska, Miroslav P Ivanov, Greta C Panova, Stoyno Stoynov
The thermodynamic patterns of eukaryotic genes suggest a mechanism for intron-exon recognition.
Nat Commun, 4 Art. No. 2101 (2013)
Open Access   DOI
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Claes Andréasson, Anna J Schick, Susanne M Pfeiffer, Mihail Sarov, Francis Stewart, Wolfgang Wurst, Joel A Schick
Direct cloning of isogenic murine DNA in yeast and relevance of isogenicity for targeting in embryonic stem cells
PLoS ONE, 8(9) Art. No. e74207 (2013)
Open Access   DOI

2012

Mihail Sarov, John I Murray, Kristin Schanze, Andrei Pozniakovski, Wei Niu, Karolin Angermann, Susanne Hasse, Michaela Rupprecht, Elisabeth Vinis, Matthew Tinney, Elicia A. Preston, Andrea Zinke, Susanne Enst, Tina Teichgraber, Judith Janette, Kadri Reis, Stephan Janosch, Siegfried Schloissnig, Radoslaw K Ejsmont, Cindie Slightam, Xiao Xu, Stuart K Kim, Valerie Reinke, A Francis Stewart, Michael Snyder, Robert H Waterston, Anthony A. Hyman
A Genome-Scale Resource for In Vivo Tag-Based Protein Function Exploration in C. elegans.
Cell, 150(4) 855-866 (2012)
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2011

Marta Nedelkova, Marcello Maresca, Jun Fu, Mariya Rostovskaya, Ramu Chenna, Christian Thiede, Konstantinos Anastassiadis, Mihail Sarov, A Francis Stewart
Targeted isolation of cloned genomic regions by recombineering for haplotype phasing and isogenic targeting.
Nucleic Acids Res, 39(20) Art. No. e137 (2011)
Open Access   DOI
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Antigoni Elefsinioti, Ömer Sinan Saraç, Anna Hegele, Conrad Plake, Nina C Hubner, Ina Poser, Mihail Sarov, Anthony A. Hyman, Matthias Mann, Michael Schroeder, Ulrich Stelzl, Andreas Beyer
Large-scale de novo prediction of physical protein-protein association.
Mol Cell Proteomics, 10(11) Art. No. M111.010629 (2011)
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Sarah C Petersen, Joseph D Watson, Janet E Richmond, Mihail Sarov, Walter W Walthall, David M Miller
A transcriptional program promotes remodeling of GABAergic synapses in Caenorhabditis elegans
J Neurosci, 31(43) 15362-15375 (2011)
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Helmut Hofemeister, Giovanni Ciotta, Jun Fu, Philipp Martin Seibert, Alexander Schulz, Marcello Maresca, Mihail Sarov, Konstantinos Anastassiadis, A. Francis Stewart
Recombineering, transfection, Western, IP and ChIP methods for protein tagging via gene targeting or BAC transgenesis.
Methods, 53(4) 437-452 (2011)
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Giovanni Ciotta, Helmut Hofemeister, Marcello Maresca, Jun Fu, Mihail Sarov, Konstantinos Anastassiadis, A Francis Stewart
Recombineering BAC transgenes for protein tagging
Methods, 53(2) 113-119 (2011)
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Wei Niu, Zhi John Lu, Mei Zhong, Mihail Sarov, James T Murray, Cathleen M Brdlik, Judith Janette, Chao Chen, Pedro Alves, Elicia A. Preston, Cindie Slightam, Lixia Jiang, Anthony A. Hyman, Stuart K Kim, Robert H Waterston, Mark Gerstein, Michael Snyder, Valerie Reinke
Diverse transcription factor binding features revealed by genome-wide ChIP-seq in C. elegans.
Genome Res, 21(2) 245-254 (2011)
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Radoslaw K Ejsmont, Peter Ahlfeld, Andrei I. Pozniakovsky, A Francis Stewart, Pavel Tomancak#, Mihail Sarov#
Recombination-mediated genetic engineering of large genomic DNA transgenes.
Methods Mol Biol, 772 445-458 (2011)
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2010

Mark Gerstein✳︎, Zhi John Lu✳︎, Eric L. Van Nostrand✳︎, Chao Cheng✳︎, Bradley I. Arshinoff✳︎, Tao Liu✳︎, Kevin Y. Yip✳︎, Rebecca Robilotto✳︎, Andreas Rechtsteiner✳︎, Kohta Ikegami✳︎, Pedro Alves✳︎, Aurelien Chateigner✳︎, Marc Perry✳︎, Mitzi Morris✳︎, Raymond Auerbach✳︎, Xin Feng✳︎, Jing Leng✳︎, Anne Vielle✳︎, Wei Niu✳︎, Kahn Rhrissorrakrai✳︎, Ashish Agarwal, Roger P. Alexander, Galt Barber, Cathleen M Brdlik, Jennifer Brennan, Jeremy Jean Brouillet, Adrian Carr, Ming-Sin Cheung, Hiram Clawson, Sergio Contrino, Luke O. Dannenberg, Abby F. Dernburg, Arshad Desai, Lindsay Dick, Andréa Dosé, Jiang Du, Thea Egelhofer, Sevinc Ercan, Ghia Euskirchen, Brent Ewing, Elise A. Feingold, Reto Gassmann, Peter J. Good, Phil Green, Francois Gullier, Michelle Gutwein, Mark S. Guyer, Lukas Habegger, Ting Han, Jorja G. Henikoff, Stefan R. Henz, Angie Hinrichs, Heather Holster, Anthony A. Hyman, A. Leo Iniguez, Judith Janette, Morten Jensen, Masaomi Kato, W. James Kent, Ellen Kephart, Vishal Khivansara, Ekta Khurana, John K. Kim, Paulina Kolasinska-Zwierz, Eric C. Lai, Isabel Latorre, Amber Leahey, Suzanna E Lewis, Paul Lloyd, Lucas Lochovsky, Rebecca F. Lowdon, Yaniv Lubling, Rachel Lyne, Michael MacCoss, Sebastian D. Mackowiak, Marco Mangone, Sheldon McKay, Desirea Mecenas, Gennifer Merrihew, David M. Miller, Andrew Muroyama, John I. Murray, Siew-Loon Ooi, Hoang Pham, Taryn Phippen, Elicia A. Preston, Nikolaus Rajewski, Gunnar Rätsch, Heidi Rosenbaum, Joel Rozowksy, Kim Rutherford, Peter Ruzanov, Mihail Sarov, Rajkumar Sasidharan, Andrea Sboner, Paul Scheid, Eran Segal, Hyunjin Shin, Chong Shou, Frank J. Slack, Cindie Slightam, Richard Smith, William C. Spencer, E. O. Stinson, Scott Taing, Teruaki Takasaki, Dionne Vafeados, Ksenia Voronina, Guilin Wang, Nicole L. Washington, Christina M. Whittle, Beijing Wu, Koon-Kiu Yan, Georg Zeller, Zheng Zha, Mei Zhong, Xingliang Zhou, Julie Ahringer, Susan Strome, Kristin C. Gunsalus, Gos Micklem, X. Shirley Liu, Valerie Reinke, Stuart K Kim, LaDeana W Hillier, Steven Henikoff, Fabio Piano, Michael Snyder, Lincoln Stein, Jason D. Lieb, Robert H Waterston
Integrative analysis of the Caenorhabditis elegans genome by the modENCODE project
Science, 330(6012) 1775-1787 (2010)
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Haiyan Lei, Tetsunari Fukushige, Wei Niu, Mihail Sarov, Valerie Reinke, Michael Krause
A widespread distribution of genomic CeMyoD binding sites revealed and cross validated by ChIP-Chip and ChIP-Seq techniques.
PLoS ONE, 5(12) Art. No. e15898 (2010)
Open Access   PDF    DOI
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James R A Hutchins✳︎, Yusuke Toyoda✳︎, Björn Hegemann✳︎, Ina Poser✳︎, Jean-Karim Hériché, Martina M Sykora, Martina Augsburg, Otto Hudecz, Bettina A Buschhorn, Jutta Bulkescher, Christian Conrad, David Comartin, Alexander Schleiffer, Mihail Sarov, Andrei I. Pozniakovsky, Mikolaj Slabicki, Siegfried Schloissnig, Ines Steinmacher, Marit Leuschner, Andrea Ssykor, Steffen Lawo, Laurence Pelletier, Holger Stark, Kim Nasmyth, Jan Ellenberg, Richard Durbin, Frank Buchholz, Karl Mechtler, Anthony A. Hyman#, Jan-Michael Peters#
Systematic analysis of human protein complexes identifies chromosome segregation proteins.
Science, 328(5978) 593-599 (2010)
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Mei Zhong, Wei Niu, Zhi John Lu, Mihail Sarov, James T Murray, Judith Janette, Debasish Raha, Karyn L Sheaffer, Hugo Y K Lam, Elicia A. Preston, Cindie Slightam, LaDeana W Hillier, Trisha Brock, Ashish Agarwal, Raymond Auerbach, Anthony A. Hyman, Mark Gerstein, Susan E Mango, Stuart K Kim, Robert H Waterston, Valerie Reinke#, Michael Snyder#
Genome-wide identification of binding sites defines distinct functions for Caenorhabditis elegans PHA-4/FOXA in development and environmental response.
PLoS Genet, 6(2) Art. No. e1000848 (2010)
Open Access   PDF    DOI

2009

Radoslaw K Ejsmont, Mihail Sarov, Sylke Winkler, Kamil A Lipinski, Pavel Tomancák
A toolkit for high-throughput, cross-species gene engineering in Drosophila.
Nat Methods, 6(6) 435-437 (2009)
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2008

Ina Poser, Mihail Sarov, James R A Hutchins, Jean-Karim Hériché, Yusuke Toyoda, Andrei I. Pozniakovsky, Daniela Weigl, Anja Nitzsche, Björn Hegemann, Alexander W. Bird, Laurence Pelletier, Ralf Kittler, Sujun Hua, Ronald Naumann, Martina Augsburg, Martina M Sykora, Helmut Hofemeister, Youming Zhang, Kim Nasmyth, Kevin P White, Steffen Dietzel, Karl Mechtler, Richard Durbin, A. Francis Stewart, Jan-Michael Peters, Frank Buchholz, Anthony A. Hyman
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.
Nat Methods, 5(5) 409-415 (2008)
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2006

Mihail Sarov, Susan Schneider, Andrei I. Pozniakovsky, Assen Roguev, Susanne Ernst, Youming Zhang, Anthony A. Hyman, A. Francis Stewart
A recombineering pipeline for functional genomics applied to Caenorhabditis elegans.
Nat Methods, 3(10) 839-844 (2006)
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Junping Wang, Mihail Sarov, Jeanette Rientjes, Jun Fu, Heike Hollak, Harald Kranz, Wei Xie, A Francis Stewart, Youming Zhang
An improved recombineering approach by adding RecA to lambda Red recombination
Mol Biotechnol, 32(1) 43-53 (2006)
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2005

Mihail Sarov, A Francis Stewart
The best control for the specificity of RNAi
Trends Biotechnol, 23(9) 446-448 (2005)
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2004

Evdokia Pasheva, Mihail Sarov, Kiril Bidjekov, Iva Ugrinova, Bettina Sarg, Herbert Lindner, Iliya G Pashev
In vitro acetylation of HMGB-1 and -2 proteins by CBP: the role of the acidic tail
Biochemistry, 43(10) 2935-2940 (2004)
DOI

2003

Daniel Schaft✳︎, Assen Roguev✳︎, Kimberly M. Kotovic, Anna Shevchenko, Mihail Sarov, Andrej Shevchenko, Karla M. Neugebauer, A. Francis Stewart
The histone 3 lysine 36 methyltransferase, SET2, is involved in transcriptional elongation.
Nucleic Acids Res, 31(10) 2475-2482 (2003)
Open Access   PDF