Technology Development Groups

* joined first author # joined corresponding author

2020

Katharina Holzhüter, Eric R Geertsma
Functional (un)cooperativity in elevator transport proteins.
Biochem Soc Trans, 48(3) 1047-1055 (2020)
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Iwan Zimmermann, Pascal Egloff, Cedric Aj Hutter, Benedikt Kuhn, Philipp Bräuer, Simon Newstead, Roger Jp Dawson, Eric R Geertsma#, Markus A Seeger#
Generation of synthetic nanobodies against delicate proteins.
Nat Protoc, 15(5) 1707-1741 (2020)
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Benedikt Kuhn, Iwan Zimmermann, Pascal Egloff, Lea M Hürlimann, Cedric Aj Hutter, Christian Miscenic, Roger Jp Dawson, Markus A Seeger#, Eric R Geertsma#
Biotinylation of Membrane Proteins for Binder Selections.
Methods Mol Biol, 2127 151-165 (2020)
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Jessica Kobylka, Miriam S Kuth, Reinke T Müller, Eric R Geertsma, Klaas M Pos
AcrB: a mean, keen, drug efflux machine.
Ann N Y Acad Sci, 1459(1) 38-68 (2020)
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2019

Susanne Hofmann✳︎, Dovile Januliene✳︎, Ahmad R Mehdipour✳︎, Christoph Thomas, Erich Stefan, Stefan Brüchert, Benedikt Kuhn, Eric R Geertsma, Gerhard Hummer, Robert Tampé#, Arne Moeller#
Conformation space of a heterodimeric ABC exporter under turnover conditions.
Nature, 571(7766) 580-583 (2019)
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Yung-Ning Chang✳︎, Eva A Jaumann✳︎, Katrin Reichel✳︎, Julia Hartmann, Dominik Oliver, Gerhard Hummer#, Benesh Joseph#, Eric R Geertsma#
Structural basis for functional interactions in dimers of SLC26 transporters.
Nat Commun, 10(1) Art. No. 2032 (2019)
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2018

Iwan Zimmermann✳︎, Pascal Egloff✳︎, Cedric Aj Hutter✳︎, Fabian M Arnold, Peter Stohler, Nicolas Bocquet, Melanie N Hug, Sylwia Huber, Martin Siegrist, Lisa Hetemann, Jennifer Gera, Samira Gmür, Peter Spies, Daniel Gygax, Eric R Geertsma#, Roger Jp Dawson#, Markus A Seeger#
Synthetic single domain antibodies for the conformational trapping of membrane proteins.
Elife, 7 Art. No. e34317 (2018)
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2017

Kathrin Koch, Sarah Kalusche, Jonathan L Torres, Robyn L Stanfield, Welbeck Danquah, Kamal Khazanehdari, Hagen von Briesen, Eric R Geertsma, Ian A Wilson, Ulrich Wernery, Friedrich Koch-Nolte, Andrew B Ward, Ursula Dietrich
Selection of nanobodies with broad neutralizing potential against primary HIV-1 strains using soluble subtype C gp140 envelope trimers.
Sci Rep, 7(1) Art. No. 8390 (2017)
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Stephan Schenck, Laura Kunz, Daniela Sahlender, Els Pardon, Eric R Geertsma, Iaroslav Savtchouk, Toshiharu Suzuki, Yvonne Neldner, Saša Štefanić, Jan Steyaert, Andrea Volterra, Raimund Dutzler
Generation and Characterization of Anti-VGLUT Nanobodies Acting as Inhibitors of Transport.
Biochemistry, 56(30) 3962-3971 (2017)
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Yung-Ning Chang, Eric R Geertsma
The novel class of seven transmembrane segment inverted repeat carriers.
Biol Chem, 398(2) 165-174 (2017)
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Jacopo Marino, Katharina Holzhüter, Benedikt Kuhn, Eric R Geertsma
Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli.
Meth Enzymol, 594 139-164 (2017)
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2015

Eric R Geertsma#, Yung-Ning Chang, Farooque R Shaik, Yvonne Neldner, Els Pardon, Jan Steyaert, Raimund Dutzler#
Structure of a prokaryotic fumarate transporter reveals the architecture of the SLC26 family.
Nat Struct Mol Biol, 22(10) 803-808 (2015)
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Magdalena A Bukowska, Michael Hohl, Eric R Geertsma, Lea M Hürlimann, Markus G Grütter, Markus A Seeger
A Transporter Motor Taken Apart: Flexibility in the Nucleotide Binding Domains of a Heterodimeric ABC Exporter.
Biochemistry, 54(19) 3086-3099 (2015)
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Jacopo Marino, Michael Hohl, Markus A Seeger, Oliver Zerbe, Eric R Geertsma
Bicistronic mRNAs to enhance membrane protein overexpression.
J Mol Biol, 427(4) 943-954 (2015)
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2014

Ines A Ehrnstorfer, Eric R Geertsma, Els Pardon, Jan Steyaert, Raimund Dutzler
Crystal structure of a SLC11 (NRAMP) transporter reveals the basis for transition-metal ion transport.
Nat Struct Mol Biol, 21(11) 990-996 (2014)
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Eric R Geertsma
FX cloning: a simple and robust high-throughput cloning method for protein expression.
Methods Mol Biol, 1116 153-164 (2014)
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2013

Eric R Geertsma
FX cloning: a versatile high-throughput cloning system for characterization of enzyme variants.
Methods Mol Biol, 978 133-148 (2013)
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2012

Jacopo Marino, Eric R Geertsma, Oliver Zerbe
Topogenesis of heterologously expressed fragments of the human Y4 GPCR.
Biochim Biophys Acta, 1818(12) 3055-3063 (2012)
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Goran Malojčić✳︎, Eric R Geertsma✳︎, Maurice S Brozzo, Rudi Glockshuber
Mechanism of the prokaryotic transmembrane disulfide reduction pathway and its in vitro reconstitution from purified components.
Angew Chem Int Ed Engl, 51(28) 6900-6903 (2012)
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2011

Eric R Geertsma#, Raimund Dutzler#
A versatile and efficient high-throughput cloning tool for structural biology.
Biochemistry, 50(15) 3272-3278 (2011)
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Ravi K R Marreddy, Joao P C Pinto, Justina C Wolters, Eric R Geertsma, Fabrizia Fusetti, Hjalmar P Permentier, Oscar P Kuipers, Jan Kok, B Poolman
The response of Lactococcus lactis to membrane protein production.
PLoS ONE, 6(8) Art. No. e24060 (2011)
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2010

Daniel M Linares, Eric R Geertsma, B Poolman
Evolved Lactococcus lactis strains for enhanced expression of recombinant membrane proteins.
J Mol Biol, 401(1) 45-55 (2010)
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Ravi K R Marreddy, Eric R Geertsma, Hjalmar P Permentier, Joao P C Pinto, Jan Kok, B Poolman
Amino acid accumulation limits the overexpression of proteins in Lactococcus lactis.
PLoS ONE, 5(4) Art. No. e10317 (2010)
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Eric R Geertsma, B Poolman
Production of membrane proteins in Escherichia coli and Lactococcus lactis.
Methods Mol Biol, 601 17-38 (2010)
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2009

Johanna M Kuiper, Radek Pluta, Wim H C Huibers, Fabrizia Fusetti, Eric R Geertsma, B Poolman
A method for site-specific labeling of multiple protein thiols.
Protein Sci, 18(5) 1033-1041 (2009)
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Christopher Mulligan✳︎, Eric R Geertsma✳︎, Emmanuele Severi, David J Kelly, B Poolman, Gavin H Thomas
The substrate-binding protein imposes directionality on an electrochemical sodium gradient-driven TRAP transporter.
Proc Natl Acad Sci U.S.A., 106(6) 1778-1783 (2009)
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2008

Eric R Geertsma, Maarten Groeneveld, Dirk Jan Slotboom, B Poolman
Quality control of overexpressed membrane proteins.
Proc Natl Acad Sci U.S.A., 105(15) 5722-5727 (2008)
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Eric R Geertsma, N A B Nik Mahmood, Gea K Schuurman-Wolters, B Poolman
Membrane reconstitution of ABC transporters and assays of translocator function.
Nat Protoc, 3(2) 256-266 (2008)
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2007

Eric R Geertsma, B Poolman
High-throughput cloning and expression in recalcitrant bacteria.
Nat Methods, 4(9) 705-707 (2007)
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Ria H Duurkens, Menno B Tol, Eric R Geertsma, Hjalmar P Permentier, Dirk Jan Slotboom
Flavin binding to the high affinity riboflavin transporter RibU.
J Biol Chem, 282(14) 10380-10386 (2007)
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B Poolman, Eric R Geertsma, Dirk Jan Slotboom
Biochemistry. A missing link in membrane protein evolution.
Science, 315(5816) 1229-1231 (2007)
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2006

Catherine M Burgess, Dirk Jan Slotboom, Eric R Geertsma, Ria H Duurkens, B Poolman, Douwe van Sinderen
The riboflavin transporter RibU in Lactococcus lactis: molecular characterization of gene expression and the transport mechanism.
J Bacteriol, 188(8) 2752-2760 (2006)
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2005

Eric R Geertsma, Ria H Duurkens, B Poolman
The activity of the lactose transporter from Streptococcus thermophilus is increased by phosphorylated IIA and the action of beta-galactosidase.
Biochemistry, 44(48) 15889-15897 (2005)
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Eric R Geertsma, Ria H Duurkens, B Poolman
Functional interactions between the subunits of the lactose transporter from Streptococcus thermophilus.
J Mol Biol, 350(1) 102-111 (2005)
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Elisa B Vervoort, Jelle B Bultema, Gea K Schuurman-Wolters, Eric R Geertsma, Jaap Broos, B Poolman
The first cytoplasmic loop of the mannitol permease from Escherichia coli is accessible for sulfhydryl reagents from the periplasmic side of the membrane.
J Mol Biol, 346(3) 733-743 (2005)
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Mark K Doeven, Joost H A Folgering, Victor Krasnikov, Eric R Geertsma, Geert van den Bogaart, B Poolman
Distribution, lateral mobility and function of membrane proteins incorporated into giant unilamellar vesicles.
Biophys J, 88(2) 1134-1142 (2005)
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B Poolman, Mark K Doeven, Eric R Geertsma, Esther Biemans-Oldehinkel, Wil N Konings, Douglas C Rees
Functional analysis of detergent-solubilized and membrane-reconstituted ATP-binding cassette transporters.
Meth Enzymol, 400 429-459 (2005)
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2003

Eric R Geertsma, Ria H Duurkens, B Poolman
Identification of the dimer interface of the lactose transport protein from Streptococcus thermophilus.
J Mol Biol, 332(5) 1165-1174 (2003)
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2000

L M Veenhoff, Eric R Geertsma, J Knol, B Poolman
Close approximation of putative alpha -helices II, IV, VII, X, and XI in the translocation pathway of the lactose transport protein of Streptococcus thermophilus.
J Biol Chem, 275(31) 23834-23840 (2000)
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