The major goal of the High-Throughput Technology Development Studio (TDS) is to provide expertise in assay development towards high-throughput applications and high content screening services (HCS). To this end the HT-TDS has state-of-the-art technologies for cell-based screening such as robotic liquid handling stations, plate readers and several automated microscopes. The services include developing complex automated image analysis pipelines and multi-parametric analysis of high content imaging applications.


Assay and technology development for high-content screening (lab automation, siRNA transfection, chemical screening, automated microscopy, live cell imaging, image analysis, data management, mutli-parametric statistics), robotic liquid handling processes (rearraying of libraries, hit picking, normalisation)


  • Application of automated image-based screening at subcellular resolution
  • Analysis of kinetic parameters of biological processes in medium-throughput mode with live cell automated microscopy
  • Identification of new gene functions and phenotypes after depletion or knock-down
  • Identification of new chemical or biological entities that induce equivalent phenotypes
  • Using chemico-genomic approaches to discover mode of action of compounds (comparison RNAi and chemical screens)


  • Automated confocal microscopy
  • Automated widefield microscopy
  • Fluorescence emission
  • Fluorescence polarisation
  • HTRF (homogeneous time resolved fluorescence)
  • Alpha ELISA
  • Luminescence
  • Absorbance
  • Acoustic dispensing
  • Electroporation
  • Automated liquid handling (contact and free dispense)


Complete execution of high-throughput / high-content screening projects

  • Assay transfer (adaptation of the bench experiment to a screenable assay)
  • Automation
  • Validation
  • Screening

Provision of individual processes performed by TDS-staff:

  • Transfection 
  • Liquid handling for all sorts of applications (reformatting & replication of libraries, normalisation of products to identical concentrations, cherry/hit picking, preparation of dilution series, etc.)
  • Measurements in homogeneous solution with plate readers
  • Measurements in cells or tissues with automated microscopes

Assay Development:

  • Assay design: full service cell-based high-content assay design and development using multi-parametric readout to tackle your specific biological questions
  • Assay optimisation: cost effective process optimisation for high-throughput screening, down-scaling, transfection optimisation, work-flow optimisationAssay validation: validation of assays according to well-established standards
  • Image and statistical analysis
  • Development of image analysis solutions for imaging projects (low and high throughput microscopy)(CellProfiler, MotionTracker, Acapella, Matlab, BioApplications)
  • Development of statistical analysis solutions for biological questions (R, KNIME, Matlab)

User Facility:

  • After an introduction and initial training, the TDS provides individuals with the opportunity to perform automated experiments or projects on their own with the instruments provided in the User Facility



  • Flexible liquid handling work stations Biomek FXp (Beckman), Freedom Evo 200 (TECAN)
  • Multilabel microtiter plate readers Envision (Perkin Elmer),  GENios Pro (TECAN)
  • Acoustic dispensing (Labcyte Echo 550)
  • CellVoyager CV7000 automated live cell confocal microscope (Yokogaw)
  • Perkin Elmer OPERA
  • Operetta widefield microscope (Perkin Elmer)
  • Automated confocal microscopy OPERA (Perkin Elmer)
  • Washers, dispensers
  • 96-well electroporation system (amaxa nucleofector)