Services & Facilities

2019

Steven W Poser, Oliver Otto, Carina Arps-Forker, Yan Ge, Maik Herbig, Cordula Andree, Konrad Gruetzmann, Melissa F Adasme, Szymon Stodolak, Polyxeni Nikolakopoulou, Deric M Park, Alan Mcintyre, Mathias Lesche, Andreas Dahl, Petra Lennig, Stefan R. Bornstein, Evelin Schroeck, Barbara Klink, Ronen R Leker, Marc Bickle, George P Chrousos, Michael Schroeder, Carlo Vittorio Cannistraci, Jochen Guck, Andreas Androutsellis-Theotokis
Controlling distinct signaling states in cultured cancer cells provides a new platform for drug discovery.
FASEB J, Art. No. doi: 10.1096/fj.201802603RR (2019)
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Ioannis Giannios, Ioannis Serafimidis, Vivian Anastasiou, Daniela Pezzolla, Mathias Lesche, Cordula Andree, Marc Bickle, Anthony Gavalas
Protein Methyltransferase Inhibition Decreases Endocrine Specification Through the Upregulation of Aldh1b1 Expression.
Stem Cells, 37(5) 640-651 (2019)
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Lydia Reinhardt, Susanne Kordes, Peter Reinhardt, Michael Glatza, Michael Baumann, Hannes C A Drexler, Sascha Menninger, Gunther Zischinsky, Jan Eickhoff, Claudia Fröb, Prabesh Bhattarai, Guruchandar Arulmozhivarman, Lara Marrone, Antje Janosch, Kenjiro Adachi, Martin Stehling, Eric D Anderson, Masin Abo-Rady, Marc Bickle, Udai Pandey, Michell M Reimer, Caghan Kizil, Hans R Schöler, Peter Nussbaumer, Bert Klebl, Jared Sterneckert
Dual Inhibition of GSK3β and CDK5 Protects the Cytoskeleton of Neurons from Neuroinflammatory-Mediated Degeneration In Vitro and In Vivo.
Stem Cell Reports, 12(3) 502-517 (2019)
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Antje Janosch, Carolin Kaffka, Marc Bickle
Unbiased Phenotype Detection Using Negative Controls.
SLAS Discov, 24(3) 234-241 (2019)
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Marc Bickle
The Academic Pill: How Academia Contributes to Curing Diseases.
SLAS Discov, 24(3) 203-212 (2019)
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Matthias Höllerhage, Marc Bickle, Günter U Höglinger
Unbiased Screens for Modifiers of Alpha-Synuclein Toxicity.
Curr Neurol Neurosci Rep, 19(2) Art. No. 8 (2019)
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Martin Stöter, Antje Janosch, Rico Barsacchi, Marc Bickle
CellProfiler and KNIME: Open-Source Tools for High-Content Screening.
Methods Mol Biol, 1953 43-60 (2019)

2018

Juliana Roscito, Katrin Sameith, Genis Parra, Bjoern Langer, Andreas Petzold, Claudia Moebius, Marc Bickle, Miguel Trefaut Rodrigues, Michael Hiller
Phenotype loss is associated with widespread divergence of the gene regulatory landscape in evolution.
Nat Commun, 9(1) Art. No. 4737 (2018)
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Neil O Carragher, Filippo Piccinini, Anna Tesei, O Joseph Trask Jr, Marc Bickle, Peter Horvath
Concerns, challenges and promises of high-content analysis of 3D cellular models.
Nat Rev Drug Discov, 17(8) Art. No. 606 (2018)
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Alvin Kuriakose Thomas, Robert Wieduwild, Ralf Zimmermann, Weilin Lin, Jens Friedrichs, Marc Bickle, Karim Fahmy, Carsten Werner, Yixin Zhang
Layer-by-Layer Assembly of Heparin and Peptide-Polyethylene Glycol Conjugates to Form Hybrid Nanothin Films of Biomatrices.
ACS Appl Mater Interfaces, 10(17) 14264-14270 (2018)
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Shovamayee Maharana, Jie Wang, Dimitrios Papadopoulos, Doris Richter, Andrei I. Pozniakovsky, Ina Poser, Marc Bickle, Sandra Rizk, Jordina Guillén-Boixet, Titus Franzmann, Marcus Jahnel, Lara Marrone, Young-Tae Chang, Jared Sterneckert, Pavel Tomancak, Anthony Hyman, Simon Alberti
RNA buffers the phase separation behavior of prion-like RNA binding proteins.
Science, 360(6391) 918-921 (2018)
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Lara Marrone, Ina Poser, Ian Casci, Julia Japtok, Peter Reinhardt, Antje Janosch, Cordula Andree, Hyun-Ok Kate Lee, Claudia Moebius, Ellen Koerner, Lydia Reinhardt, Maria Elena Cicardi, Karl Hackmann, Barbara Klink, Angelo Poletti, Simon Alberti, Marc Bickle, Andreas Hermann, Udai Pandey, Anthony Hyman, Jared Sterneckert
Isogenic FUS-eGFP iPSC Reporter Lines Enable Quantification of FUS Stress Granule Pathology that Is Rescued by Drugs Inducing Autophagy.
Stem Cell Reports, 10(2) 375-389 (2018)
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Milan Esner, Felix Meyenhofer, Marc Bickle
Live-Cell High Content Screening in Drug Development.
Methods Mol Biol, 1683 149-164 (2018)

2017

Guruchandar Arulmozhivarman, Martin Kräter, Manja Wobus, Jens Friedrichs, Elham Pishali Bejestani, Katrin Müller, Katrin Lambert, Dimitra Alexopoulou, Andreas Dahl, Martin Stöter, Marc Bickle, Nona Shayegi, Jochen Hampe, Friedrich Stölzel, Michael Brand, Malte von Bonin, Martin Bornhäuser
Zebrafish In-Vivo Screening for Compounds Amplifying Hematopoietic Stem and Progenitor Cells: - Preclinical Validation in Human CD34+ Stem and Progenitor Cells.
Sci Rep, 7(1) Art. No. 12084 (2017)
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Matthias Höllerhage, Claudia Moebius, Johannes Melms, Wei-Hua Chiu, Joachim N Goebel, Tasnim Chakroun, Thomas Koeglsperger, Wolfgang H Oertel, Thomas W Rösler, Marc Bickle, Günter U Höglinger
Protective efficacy of phosphodiesterase-1 inhibition against alpha-synuclein toxicity revealed by compound screening in LUHMES cells.
Sci Rep, 7(1) Art. No. 11469 (2017)
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J Gray Camp, Keisuke Sekine, Tobias Gerber, Henry Loeffler-Wirth, Hans Binder, Malgorzata Gac, Sabina Kanton, Jorge Kageyama, Georg Damm, Daniel Seehofer, Lenka Belicova, Marc Bickle, Rico Barsacchi, Ryo Okuda, Emi Yoshizawa, Masashi Kimura, Hiroaki Ayabe, Hideki Taniguchi, Takanori Takebe, Barbara Treutlein
Multilineage communication regulates human liver bud development from pluripotency.
Nature, 546(7659) 533-538 (2017)

2016

Peter Horvath, Nathalie Aulner, Marc Bickle, Anthony M Davies, Elaine Del Nery, Daniel Ebner, Maria Montoya, Päivi Östling, Vilja Pietiäinen, Leo S Price, Spencer L Shorte, Gerardo Turcatti, Carina von Schantz, Neil O Carragher
Screening out irrelevant cell-based models of disease.
Nat Rev Drug Discov, 15(11) 751-769 (2016)
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Maria Montoya, Thierry Dorval, Marc Bickle
SLAS Europe High-Content Screening Conference in Dresden: A Glimpse of the Future?
J Biomol Screen, 21(9) 883-886 (2016)
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Guruchandar Arulmozhivarman, Martin Stöter, Marc Bickle, Martin Kräter, Manja Wobus, Gerhard Ehninger, Friedrich Stölzel, Michael Brand, Martin Bornhäuser, Nona Shayegi
In Vivo Chemical Screen in Zebrafish Embryos Identifies Regulators of Hematopoiesis Using a Semiautomated Imaging Assay.
J Biomol Screen, 21(9) 956-964 (2016)
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Susanne Sales, Juergen Graessler, Sara Ciucci, Rania Al-Atrib, Terhi Vihervaara, Kai Schuhmann, Dimple Kauhanen, Marko Sysi-Aho, Stefan Bornstein, Marc Bickle, Carlo Vittorio Cannistraci, Kim Ekroos, Andrej Shevchenko
Gender, Contraceptives and Individual Metabolic Predisposition Shape a Healthy Plasma Lipidome.
Sci Rep, 6 Art. No. 27710 (2016)
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Jon M Carthy, Martin Stöter, Claudia Bellomo, Michael Vanlandewijck, Angelos Heldin, Anita Morén, Dimitris Kardassis, Timothy C Gahman, Andrew K Shiau, Marc Bickle, Marino Zerial, Carl-Henrik Heldin, Aristidis Moustakas
Chemical regulators of epithelial plasticity reveal a nuclear receptor pathway controlling myofibroblast differentiation.
Sci Rep, 6 Art. No. 29868 (2016)

2015

Jerome Gilleron, Prasath Paramasivam, Anja Zeigerer, William Querbes, Giovanni Marsico, Cordula Andree, Sarah Seifert, Pablo Amaya, Martin Stöter, Victor Koteliansky, Herbert Waldmann, Kevin Fitzgerald, Yannis Kalaidzidis, Akin Akinc, Martin A Maier, Muthiah Manoharan, Marc Bickle, Marino Zerial
Identification of siRNA delivery enhancers by a chemical library screen.
Nucleic Acids Res, 43(16) 7984-8001 (2015)
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Marc Bickle, Hakim Djaballah, Lorenz Martin Mayr
The King Is Dead, Long Live the King! JBS Special Issue on Screening by RNAi and Precise Genome Editing Technologies.
J Biomol Screen, 20(8) 929-931 (2015)
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Marisa P. McShane, Tim Friedrichson, Angelika Giner, Felix Meyenhofer, Rico Barsacchi, Marc Bickle, Marino Zerial
A Combination of Screening and Computational Approaches for the Identification of Novel Compounds That Decrease Mast Cell Degranulation.
J Biomol Screen, 20(6) 720-728 (2015)
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Anne Eugster, Aaron Lindner, Mara Catani, Anne-Kristin Heninger, Andreas Dahl, Sylvia Klemroth, Denise Kühn, Susanne M Dietz, Marc Bickle, Anette-Gabrielle Ziegler, Enzio Bonifacio
High Diversity in the TCR Repertoire of GAD65 Autoantigen-Specific Human CD4+ T Cells.
J Immunol, 194(6) 2531-2538 (2015)

2014

Mihaela Anitei, Ramu Chenna, Cornelia Czupalla, Milan Esner, Sara Christ, Steffi Lenhard, Kerstin Korn, Felix Meyenhofer, Marc Bickle, Marino Zerial, Bernard Hoflack
A high-throughput siRNA screen identifies genes that regulate mannose 6-phosphate receptor trafficking.
J Cell Sci, 127(23) 5079-5092 (2014)
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Marc Bickle
The Technology Development Studio of the MPI-CBG: An Open Access Cell-Based Screening Facility.
Comb Chem High Throughput Screen, 17(4) 322-327 (2014)
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Carsten Wenzel, Björn Riefke, Stephan Gründemann, Alice Krebs, Sven Christian, Florian Prinz, Marc Osterland, Sven Golfier, Sebastian Räse, Nariman Ansari, Milan Esner, Marc Bickle, Francesco Pampaloni, Christian Mattheyer, Ernst H K Stelzer, Karsten Parczyk, Stefan Prechtl, Patrick Steigemann
3D high-content screening for the identification of compounds that target cells in dormant tumor spheroid regions.
Exp Cell Res, 323(1) 131-143 (2014)
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Danielle Borg, Marc Weigelt, Carmen Wilhelm, Michael Gerlach, Marc Bickle, Stephan Speier, Enzio Bonifacio, Angela Hommel
Mesenchymal stromal cells improve transplanted islet survival and islet function in a syngeneic mouse model.
Diabetologia, 57(3) 522-531 (2014)
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Milan Esner, Felix Meyenhofer, Michael Kuhn, Melissa Thomas, Yannis Kalaidzidis, Marc Bickle
Development of a Kinetic Assay for Late Endosome Movement.
J Biomol Screen, 19(7) 1070-1078 (2014)
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Varadharajan Sundaramurthy, Rico Barsacchi, Mikhail Chernykh, Martin Stöter, Nadine Tomschke, Marc Bickle, Yannis Kalaidzidis, Marino Zerial
Deducing the mechanism of action of compounds identified in phenotypic screens by integrating their multiparametric profiles with a reference genetic screen.
Nat Protoc, 9(2) 474-490 (2014)

2013

Anna Ivanova, Yannis Kalaidzidis, Ronald Dirkx, Mihail Sarov, Michael Gerlach, Britta Schroth-Diez, Andreas Müller, Yanmei Liu, Cordula Andree, Bernard Mulligan, Carla Münster, Thomas Kurth, Marc Bickle, Stephan Speier, Konstantinos Anastassiadis, Michele Solimena
Age-dependent labeling and imaging of insulin secretory granules.
Diabetes, 62(11) 3687-3696 (2013)
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Esther Zanin, Arshad Desai, Ina Poser, Yusuke Toyoda, Cordula Andree, Claudia Moebius, Marc Bickle, Barbara Conradt, Alisa Piekny, Karen Oegema
A conserved RhoGAP limits M phase contractility and coordinates with microtubule asters to confine RhoA during cytokinesis.
Dev Cell, 26(5) 496-510 (2013)
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Jerome Gilleron, William Querbes, Anja Zeigerer, Anna Borodovsky, Giovanni Marsico, Undine Schubert, Kevin Manygoats, Sarah Seifert, Cordula Andree, Martin Stöter, Hila Epstein-Barash, Ligang Zhang, Victor Koteliansky, Kevin Fitzgerald, Eugenio Fava, Marc Bickle, Yannis Kalaidzidis, Akin Akinc, Martin A Maier, Marino Zerial
Image-based analysis of lipid nanoparticle-mediated siRNA delivery, intracellular trafficking and endosomal escape.
Nat Biotechnol, 31(7) 638-646 (2013)
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Varadharajan Sundaramurthy, Rico Barsacchi, Nikolay Samusik, Giovanni Marsico, Jerome Gilleron, Inna Kalaidzidis, Felix Meyenhofer, Marc Bickle, Yannis Kalaidzidis, Marino Zerial
Integration of chemical and RNAi multiparametric profiles identifies triggers of intracellular mycobacterial killing.
Cell Host Microbe, 13(2) 129-142 (2013)
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Martin Stöter, Antje Niederlein, Rico Barsacchi, Felix Meyenhofer, Holger Brandl, Marc Bickle
CellProfiler and KNIME: open source tools for high content screening.
Methods Mol Biol, 986 105-122 (2013)

2012

Marc Bickle
Systems drug discovery: a quantitative, objective approach for safer drug development.
Expert Opin Drug Discov, 7(9) 757-759 (2012)
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Julia Franziska Winter, Sebastian Höpfner, Kerstin Korn, Benjamin Farnung, Charles R. Bradshaw, Giovanni Marsico, Michael Volkmer, Bianca Habermann, Marino Zerial
Caenorhabditis elegans screen reveals role of PAR-5 in RAB-11-recycling endosome positioning and apicobasal cell polarity.
Nat Cell Biol, 14(7) 666-676 (2012)
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Eugenio Fava, J Dehghany, Joke Ouwendijk, A Müller, Antje Niederlein, Paul Verkade, M Meyer-Hermann, Michele Solimena
Novel standards in the measurement of rat insulin granules combining electron microscopy, high-content image analysis and in silico modelling.
Diabetologia, 55(4) 1013-1023 (2012)

2011

Andreas Ettinger, Michaela Wilsch-Bräuninger, Anne-Marie Marzesco, Marc Bickle, Annett Lohmann, Zoltan Maliga, Jana Karbanová, Denis Corbeil, Anthony A. Hyman, Wieland B. Huttner
Proliferating versus differentiating stem and cancer cells exhibit distinct midbody-release behaviour.
Nat Commun, 2 Art. No. 503 (2011)

2010

Marc Bickle
The beautiful cell: high-content screening in drug discovery.
Anal Bioanal Chem, 398(1) 219-226 (2010)
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Akin Akinc, William Querbes, Soma De, June Qin, Maria Frank-Kamenetsky, K Narayanannair Jayaprakash, Muthusamy Jayaraman, Kallanthottathil G Rajeev, William L Cantley, J Robert Dorkin, James S Butler, Liuliang Qin, Timothy Racie, Andrew Sprague, Eugenio Fava, Anja Zeigerer, Michael J Hope, Marino Zerial, Dinah W Y Sah, Kevin Fitzgerald, Mark A Tracy, Muthiah Manoharan, Victor Koteliansky, Antonin de Fougerolles, Martin A Maier
Targeted delivery of RNAi therapeutics with endogenous and exogenous ligand-based mechanisms.
Mol Ther, 18(7) 1357-1364 (2010)
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Hesso Farhan, Markus W Wendeler, Sandra Mitrovic, Eugenio Fava, Yael Silberberg, Roded Sharan, Marino Zerial, Hans-Peter Hauri
MAPK signaling to the early secretory pathway revealed by kinase/phosphatase functional screening.
J Cell Biol, 189(6) 997-1011 (2010)
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Claudio Collinet, Martin Stöter, Charles R. Bradshaw, Nikolay Samusik, Jochen Rink, Denise Kenski, Bianca Habermann, Frank Buchholz, Robert Henschel, Matthias S Mueller, Wolfgang E Nagel, Eugenio Fava, Yannis Kalaidzidis, Marino Zerial
Systems survey of endocytosis by multiparametric image analysis.
Nature, 464(7286) 243-249 (2010)

2009

Antje Niederlein, Felix Meyenhofer, Dan White, Marc Bickle
Image Analysis in High-Content Screening.
Comb Chem High Throughput Screen, 12(9) 899-907 (2009)
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Jesper B Bramsen, Maria B Laursen, Anne F Nielsen, Thomas B Hansen, Claus Bus, Niels Langkjaer, B Ravindra Babu, Torben Højland, Mikhail Abramov, Arthur Van Aerschot, Dalibor Odadzic, Romualdas Smicius, Jens Haas, Cordula Andree, Jharna Barman, Malgorzata Wenska, Puneet Srivastava, Chuanzheng Zhou, Dmytro Honcharenko, Simone Hess, Elke Müller, Georgii V Bobkov, Sergey N Mikhailov, Eugenio Fava, Thomas F Meyer, Jyoti Chattopadhyaya, Marino Zerial, Joachim W Engels, Piet Herdewijn, Jesper Wengel, Jørgen Kjems
A large-scale chemical modification screen identifies design rules to generate siRNAs with high activity, high stability and low toxicity.
Nucleic Acids Res, 37(9) 2867-2881 (2009)

2008

Vinciane Grimard, Julia Massier, Doris Richter, Dominik Schwudke, Yannis Kalaidzidis, Eugenio Fava, Albin Hermetter, Christoph Thiele
siRNA screening reveals JNK2 as an evolutionary conserved regulator of triglyceride homeostasis.
J Lipid Res, 49(11) 2427-2440 (2008)
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Marc Bickle
High-content screening: a new primary screening tool?
IDrugs, 11(11) 822-826 (2008)
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Marc Bickle
Live cell high content screening in drug development
European Pharmaceutical Review, 13(4) 54-60 (2008)
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Kerstin Korn, Eberhard Krausz
High-content siRNA screening for target identification and validation
Expert Opin Drug Discov, 3(5) 551-564 (2008)

2007

Kerstin Korn, Eberhard Krausz
Cell-based high-content screening of small-molecule libraries.
Curr Opin Chem Biol, 11(5) 503-510 (2007)
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Torben Lessmann, Michele G Leuenberger, Sascha Menninger, Meritxell Lopez-Canet, Oliver Müller, Stefan Hümmer, Jenny Bormann, Kerstin Korn, Eugenio Fava, Marino Zerial, Thomas U Mayer, Herbert Waldmann
Natural product-derived modulators of cell cycle progression and viral entry by enantioselective oxa Diels-Alder reactions on the solid phase.
Chem Biol, 14(4) 443-451 (2007)

2006

Anne-Kristin Heninger, Karol Kozak, Ralf Kittler, Jan Wagner, Annett Lohmann, Ina Poser, Hannes Grabner, Eberhard Krausz, Frank Buchholz
RNAi libraries for Funtional Genomics: Avoid the Difficulties of Predicting Efficient siRNAs and the Cost of Chemical Synthesis
Genetic Engineering News, 26(19) 22-23 (2006)
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Eberhard Krausz
Challenges in High-Content siRNA Screening
European Pharmaceutical Review, 6 13-20 (2006)

2005

Ayano Satoh, Jörg Malsam, Graham Warren
Tethering assays for COPI vesicles mediated by golgins
In: GTPases regulating membrane dynamics. (Eds.) William E. Balch Methods in enzymology ; 404.,Amsterdam, Netherlands,Elsevier (2005),125-134 Ch. 13
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Eberhard Krausz
Linking Multi-parametric Cell-based Assays to HTS
Screening (Darmstadt), 6 22-24 (2005)
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Christoph Sachse, Eberhard Krausz, Andrea Krönke, Michael Hannus, Andrew Walsh, Anne Grabner, Dmitriy Ovcharenko, David Dorris, Claude Trudel, Birte Sönnichsen, Christophe J. Echeverri
High throughput RNA interference strategies for target discovery and validation using syntheic short interfering RNAs: functional genomics investigations of biological pathways
In: RNA interference. (Eds.) David R. Engelke, John J. Rossi Methods in enzymology ; 392.,Amsterdam, Netherlands,Elsevier (2005),242-277 Ch. 15
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Eberhard Krausz, Lucas Pelkmans, Eugenio Fava, Michael Hannus, Claudia Möbius, Kerstin Korn, Francoise Halley, Annett Lohmann, Hannes Grabner, Marino Zerial
Genomweite funktionelle Analyse des Kinoms mittels RNA-Interfernz
BIOspektrum, 11(SH) 518-520 (2005)

2003

Eberhard Krausz, Anne Grabner, Andrea Kroenke, Christoph Sachse, Christophe J. Echeverri
Optimizing High Throughput RNAi-Based Assays Using Transient Transfection of Synthetic siRNAs in Cultured Mammalian Cells
In: RNA Interference (RNAi) - Nuts & Bolts of RNAi Technology. (Eds.) David R. Engelke,Eagleville, USA,DNA Press (2003),131-168