Services & Facilities

The Gene Expression Facility/DNA Microarray Facility currently provides service for two commercially available DNA microarray platforms: Agilent and Affymetrix. The Agilent hybridization oven and microarray confocal laser scanner set-up of the Microarray Facility allows for analysis of other 1x3” glass slide array platforms, also.  Such platforms include but are not limited to, GE Healthcare, Illumina, or custom printed arrays.  Any type of microarray, e.g. protein, peptide, antibody, lipid, glycan, etc. can be processed in the Microarray Facility. 

We have expertise in designing custom microarrays, for example DNA Microarrays with the Agilent database eArray. We offer full service of microarray experiments including sample quality control, experimental design, execution of the experiments, data analysis and data storage. For the validation of DNA microarray results, we are equipped and experienced with qRT-PCR analysis. In addition, we offer analysis service for any type of qRT-PCR projects, including sample quality control, experimental design, execution of the experiments, data analysis and data storage.

Services

  • Microarray gene expression analysis (1 color and 2 color)
  • qRT-PCR analysis
  • ChIP-on-chip analysis
  • Array-based comparative genomic hybridization
  • miRNA expression analysis
  • Any type of array processing, e.g. protein, peptide, antibody, lipid, glycan, etc.
  • Validation of DNA microarray results with qRT-PCR 
  • Custom array design, e.g. with eArray tool

Expertise

  • Comprehensive microarray proficiency
  • Comprehensive qRT-PCR proficiency
  • Professional support, teaching, and training
  • High throughput screening and large-scale data analysis
  • Experimental design and assay development
  • Design and analysis of custom microarrays, such as DNA, protein, peptide, antibody, lipid, glycan, etc.

Equipment:

  • Agilent microarray confocal laser scanner
  • Agilent hybridization oven
  • Agilent Bioanalyzer
  • Stratagene Mx 3000P qRT-PCR machines
  • Large-scale data analysis platform:  GeneSpring