Services & Facilities

The FACS Facility provides instrumentation, education and expertise for all your flow cytometry needs. The flow cytometry services include both, multi-parameter analysis and sorting of cell populations based on fluorescence labels and on phenotypic and biochemical properties of the cells.

Services:

•    Expertise to help users with experimental design and protocol development
•    Analysis and sorting of cell lines and primary tissue cells from various model organisms
•    User training on the Calibur Analyzer instrument

 

Analysis:

•    Multi-parameter analysis of cell suspensions (up to 20 parameters with 5 lasers (375, 405, 488, 561, 633nm)
•    Cell cycle analysis
•    Cell viability and apoptosis
•    Analysis of fluorescently labeled cell lines and antibody stainings

 

Cell sorting:

•    Sterile cell sorting of up to 4 subpopulations simultaneously
•    Sterile single-cell sorting into multiwell-plates (24, 48, 96 and 384 wells)

 

Equipment:

•    FacsARIA III, cell sorter, Beckton Dickinson, 4 laser lines (375/405, 488, 561 and 633 nm); 4-way sterile cell sorting into tubes or single-cell sorting into multiwell-plates.
•    FacsAria Fusion, cell sorter, Beckton Dickinson, 4 laser lines (375/405, 488, 561 and 633 nm); 4-way sterile cell sorting into tubes or single-cell sorting into multiwell-plates.
•    Calibur Analyzer, Beckton Dickinson, 2 laser lines (488 and 633 nm)