Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. With biobeam, we present an open-source software package that enables to simulate operational light-sheet microscopes by combining data from 10^5 - 10^6 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.

Biobeam will be made available as an open-source, hardware-agnostic python implementation, ready for anyone to use.
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Biobeam is developed by: Martin Weigert, Gene Myers, and Moritz Kreysing