Research Groups



Ivan Ivanov, Rafael B. Lira, T-Y Dora Tang, Titus Franzmann, Adam Klosin, Lucas Caire da Silva, Anthony Hyman, Katharina Landfester, Reinhard Lipowsky, Kai Sundmacher, Rumiana Dimova
Directed Growth of Biomimetic Microcompartments
Adv. Biosys., Art. No. doi: 10.1002/adbi.201800314 (2019)
Simon Alberti, Amy Gladfelter, Tanja Mittag
Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates.
Cell, 176(3) 419-434 (2019)
Titus Franzmann, Simon Alberti
Protein Phase Separation as a Stress Survival Strategy.
Cold Spring Harb Perspect Biol, Art. No. doi:10.1101/cshperspect.a034058 (2019)


Simon Alberti, Serena Carra
Quality Control of Membraneless Organelles.
J Mol Biol, 430(23) 4711-4729 (2018)
Simon Alberti, Shambaditya Saha, Jeffrey Woodruff, Titus Franzmann, Jie Wang, Anthony Hyman
A User's Guide for Phase Separation Assays with Purified Proteins.
J Mol Biol, 430(23) 4806-4820 (2018)
Edgar Boczek, Simon Alberti
Phase changes in neurotransmission.
Science, 361(6402) 548-549 (2018)
Jie Wang, Jeong-Mo Choi, Alex S Holehouse, Hyun O. Lee, Xiaojie Zhang, Marcus Jahnel, Shovamayee Maharana, Regis P. Lemaitre, Andrei I. Pozniakovsky, David N. Drechsel, Ina Poser, Rohit V Pappu, Simon Alberti, Anthony Hyman
A Molecular Grammar Governing the Driving Forces for Phase Separation of Prion-like RNA Binding Proteins.
Cell, 174(3) 688-699 (2018)
Titus Franzmann, Simon Alberti
Prion-like low-complexity sequences: Key regulators of protein solubility and phase behavior.
J Biol Chem, Art. No. doi: 10.1074/jbc.TM118.001190 (2018)
Sonja Kroschwald, Matthias Munder, Shovamayee Maharana, Titus Franzmann, Doris Richter, Martine Ruer, Anthony Hyman, Simon Alberti
Different Material States of Pub1 Condensates Define Distinct Modes of Stress Adaptation and Recovery.
Cell Rep, 23(11) 3327-3339 (2018)
Steven Boeynaems, Simon Alberti, Nicolas L Fawzi, Tanja Mittag, Magdalini Polymenidou, Frederic Rousseau, Joost Schymkowitz, James Shorter, Benjamin Wolozin, Ludo Van Den Bosch, Peter Tompa, Monika Fuxreiter
Protein Phase Separation: A New Phase in Cell Biology.
Trends Cell Biol, 28(6) 420-435 (2018)
Shovamayee Maharana, Jie Wang, Dimitrios Papadopoulos, Doris Richter, Andrei I. Pozniakovsky, Ina Poser, Marc Bickle, Sandra Rizk, Jordina Guillén-Boixet, Titus Franzmann, Marcus Jahnel, Lara Marrone, Young-Tae Chang, Jared Sterneckert, Pavel Tomancak, Anthony Hyman, Simon Alberti
RNA buffers the phase separation behavior of prion-like RNA binding proteins.
Science, 360(6391) 918-921 (2018)
Edgar Boczek, Simon Alberti
One domain fits all: Using disordered regions to sequester misfolded proteins.
J Cell Biol, 217(4) 1173-1175 (2018)
Matthäus Mittasch, Peter Gross, Michael Nestler, Anatol W Fritsch, Christiane Iserman, Mrityunjoy Kar, Matthias Munder, Axel Voigt, Simon Alberti, Stephan W. Grill, Moritz Kreysing
Non-invasive perturbations of intracellular flow reveal physical principles of cell organization.
Nat Cell Biol, 20(3) 344-351 (2018)
Lara Marrone, Ina Poser, Ian Casci, Julia Japtok, Peter Reinhardt, Antje Janosch, Cordula Andree, Hyun-Ok Kate Lee, Claudia Moebius, Ellen Koerner, Lydia Reinhardt, Maria Elena Cicardi, Karl Hackmann, Barbara Klink, Angelo Poletti, Simon Alberti, Marc Bickle, Andreas Hermann, Udai Pandey, Anthony Hyman, Jared Sterneckert
Isogenic FUS-eGFP iPSC Reporter Lines Enable Quantification of FUS Stress Granule Pathology that Is Rescued by Drugs Inducing Autophagy.
Stem Cell Reports, 10(2) 375-389 (2018)
Matthäus Mittasch, Peter Gross, Michael Nestler, Anatol W Fritsch, Christiane Iserman, Mrityunjoy Kar, Matthias Munder, Axel Voigt, Simon Alberti, Stephan W. Grill, Moritz Kreysing
How to apply FLUCS in single cells and living embryos
Protocol Exchange, Art. No. doi:10.1038/protex.2017.157 (2018)
Simon Alberti
Guilty by Association: Mapping Out the Molecular Sociology of Droplet Compartments.
Mol Cell, 69(3) 349-351 (2018)
Titus Franzmann, Marcus Jahnel, Andrei I. Pozniakovsky, Julia Mahamid, Alex S Holehouse, Elisabeth Nüske, Doris Richter, Wolfgang Baumeister, Stephan W. Grill, Rohit V Pappu, Anthony A. Hyman, Simon Alberti
Phase separation of a yeast prion protein promotes cellular fitness.
Science, 359(6371) Art. No. eaao5654 (2018)


Simon Alberti
Phase separation in biology.
Curr Biol, 27(20) 1097-1102 (2017)
Kamran Rizzolo, Jennifer Huen, Ashwani Kumar, Sadhna Phanse, James Vlasblom, Yoshito Kakihara, Hussein A Zeineddine, Zoran Minic, Jamie Snider, Wen Wang, Carles Pons, Thiago V Seraphim, Edgar Boczek, Simon Alberti, Michael Costanzo, Chad L Myers, Igor Stagljar, Charles Boone, Mohan Babu, Walid A Houry
Features of the Chaperone Cellular Network Revealed through Systematic Interaction Mapping.
Cell Rep, 20(11) 2735-2748 (2017)
Amayra Hernández-Vega, Marcus Braun, Lara Scharrel, Marcus Jahnel, Susanne Wegmann, Bradley T Hyman, Simon Alberti, Stefan Diez, Anthony Hyman
Local Nucleation of Microtubule Bundles through Tubulin Concentration into a Condensed Tau Phase.
Cell Rep, 20(10) 2304-2312 (2017)
Simon Alberti
The wisdom of crowds: regulating cell function through condensed states of living matter.
J Cell Sci, 130(17) 2789-2796 (2017)
Federica F Morelli, Dineke S Verbeek, Jessika Bertacchini, Jonathan Vinet, Laura Mediani, Sandra Marmiroli, Giovanna Cenacchi, Milena Nasi, Sara De Biasi, Jeanette F Brunsting, Jan Lammerding, Elena Pegoraro, Corrado Angelini, Rossella Tupler, Simon Alberti, Serena Carra
Aberrant Compartment Formation by HSPB2 Mislocalizes Lamin A and Compromises Nuclear Integrity and Function.
Cell Rep, 20(9) 2100-2115 (2017)
Serena Carra, Simon Alberti, Patrick A Arrigo, Justin L Benesch, Ivor J Benjamin, Wilbert Boelens, Britta Bartelt-Kirbach, Bianca J J M Brundel, Johannes Buchner, Bernd Bukau, John A Carver, Heath Ecroyd, Cecilia Emanuelsson, Stephanie Finet, Nikola Golenhofen, Pierre Goloubinoff, Nikolai Gusev, Martin Haslbeck, Lawrence E Hightower, Harm H Kampinga, Rachel E Klevit, Krzysztof Liberek, Hassane S Mchaourab, Kathryn A McMenimen, Angelo Poletti, Roy Quinlan, Sergei V Strelkov, Melinda E Toth, Elizabeth Vierling, Robert M Tanguay
The growing world of small heat shock proteins: from structure to functions.
Cell Stress Chaperones, 22(4) 601-611 (2017)
Avinash Patel, Liliana Malinovska, Shambaditya Saha, Jie Wang, Simon Alberti, Yamuna Krishnan, Anthony Hyman
ATP as a biological hydrotrope.
Science, 356(6339) 753-756 (2017)
Daniel Mateju, Titus Franzmann, Avinash Patel, Andrii Kopach, Edgar Boczek, Shovamayee Maharana, Hyun-Ok Kate Lee, Serena Carra, Anthony Hyman, Simon Alberti
An aberrant phase transition of stress granules triggered by misfolded protein and prevented by chaperone function.
EMBO J, 36(12) 1669-1687 (2017)
Catherine Rabouille, Simon Alberti
Cell adaptation upon stress: the emerging role of membrane-less compartments.
Curr Opin Cell Biol, 47 34-42 (2017)
Sonja Kroschwald, Simon Alberti
Gel or Die: Phase Separation as a Survival Strategy.
Cell, 168(6) 947-948 (2017)
Simon Alberti, Daniel Mateju, Laura Mediani, Serena Carra
Granulostasis: Protein Quality Control of RNP Granules.
Front Mol Neurosci, 10 Art. No. 84 (2017)


Liliana Malinovska, Simon Alberti
Studying the Protein Quality Control System of D. discoideum Using Temperature-controlled Live Cell Imaging.
J Vis Exp, (118) Art. No. e54730 (2016)
Simon Alberti, Anthony Hyman
Are aberrant phase transitions a driver of cellular aging?
Bioessays, 38(10) 959-968 (2016)
Massimo Ganassi, Daniel Mateju, Ilaria Bigi, Laura Mediani, Ina Poser, Hyun-Ok Kate Lee, Samuel J Seguin, Federica F Morelli, Jonathan Vinet, Giuseppina Leo, Orietta Pansarasa, Cristina Cereda, Angelo Poletti, Simon Alberti, Serena Carra
A Surveillance Function of the HSPB8-BAG3-HSP70 Chaperone Complex Ensures Stress Granule Integrity and Dynamism.
Mol Cell, 63(5) 796-810 (2016)
Elvan Boke, Martine Ruer, Martin Wühr, Margaret Coughlin, Regis P. Lemaitre, Steven Gygi, Simon Alberti, David N. Drechsel, Anthony Hyman, Timothy J. Mitchison
Amyloid-like Self-Assembly of a Cellular Compartment.
Cell, 166(3) 637-650 (2016)
Matthias Munder, Daniel Midtvedt, Titus Franzmann, Elisabeth Nüske, Oliver Otto, Maik Herbig, Elke Ulbricht, Paul Müller, Anna Taubenberger, Shovamayee Maharana, Liliana Malinovska, Doris Richter, Jochen Guck, Vasily Zaburdaev, Simon Alberti
A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy.
Elife, 5 Art. No. e09347 (2016)


Matthias Munder
pH - a key regulator of cellular dormacy?
Ph.D. Thesis,Technische Universität Dresden, Dresden, Germany (2015)
Liliana Malinovska, Simon Alberti
Protein misfolding in Dictyostelium: Using a freak of nature to gain insight into a universal problem.
Prion, 9(5) 339-346 (2015)
Avinash Patel, Hyun-Ok Kate Lee, Louise Jawerth, Shovamayee Maharana, Marcus Jahnel, Marco Y Hein, Stoyno Stoynov, Julia Mahamid, Shambaditya Saha, Titus Franzmann, Andrei Pozniakovski, Ina Poser, Nicola Maghelli, Loic Royer, Martin Weigert, Eugene W Myers, Stephan W. Grill, David N. Drechsel, Anthony Hyman, Simon Alberti
A Liquid-to-Solid Phase Transition of the ALS Protein FUS Accelerated by Disease Mutation.
Cell, 162(5) 1066-1077 (2015)
Simon Alberti
Don't Go with the Cytoplasmic Flow.
Dev Cell, 34(4) 381-382 (2015)
Liliana Malinovska, Sandra Palm, Kimberley Gibson, Jean-Marc Verbavatz, Simon Alberti
Dictyostelium discoideum has a highly Q/N-rich proteome and shows an unusual resilience to protein aggregation.
Proc Natl Acad Sci U.S.A., 112(20) 2620-2629 (2015)
Sonja Kroschwald
Promiscuous interactions and redundant assembly pathways promote RNP granule formation in Saccharomyces cerevisiae
Ph.D. Thesis,Technische Universität Dresden, Dresden, Germany (2015)
Sonja Kroschwald, Shovamayee Maharana, Daniel Mateju, Liliana Malinovska, Elisabeth Nüske, Ina Poser, Doris Richter, Simon Alberti
Promiscuous interactions and protein disaggregases determine the material state of stress-inducible RNP granules.
Elife, 4 Art. No. e06807 (2015)


Miguel Coelho, Steven Lade, Simon Alberti, Thilo Gross, Iva M. Tolic
Fusion of Protein Aggregates Facilitates Asymmetric Damage Segregation.
PLoS Biol, 12(6) Art. No. e1001886 (2014)
Christian Rogon, Anna Ulbricht, Michael Hesse, Simon Alberti, Preethi Vijayaraj, Diana Best, Ian R Adams, Thomas M Magin, Bernd K Fleischmann, Jörg Höhfeld
HSP70 Binding Protein HSPBP1 Regulates Chaperone Expression at a Posttranslational Level and is Essential for Spermatogenesis.
Mol Biol Cell, 25(15) 2260-2271 (2014)
Liliana Malinovska
Specific adaptations in the proteostasis network of the social amoebae Dictyostelium discoideum lead to an unusual resilience to protein aggregation
Ph.D. Thesis,Technische Universität Dresden, Dresden, Germany (2014)
Ivana Petrovska, Elisabeth Nüske, Matthias Munder, Gayathrie Kulasegaran, Liliana Malinovska, Sonja Kroschwald, Doris Richter, Karim Fahmy, Kimberley Gibson, Jean-Marc Verbavatz, Simon Alberti
Filament formation by metabolic enzymes is a specific adaptation to an advanced state of cellular starvation.
Elife, 3 Art. No. e02409 (2014)


Miguel Coelho, Aygul Dereli, Anett Haese, Sebastian Kühn, Liliana Malinovska, Morgan E Desantis, James Shorter, Simon Alberti, Thilo Gross, Iva M. Tolic-Norrelykke
Fission Yeast Does Not Age under Favorable Conditions, but Does So after Stress.
Curr Biol, 23(19) 1844-1852 (2013)
Simon Alberti
Aggregating the message to control the cell cycle.
Dev Cell, 25(6) 551-552 (2013)
Liliana Malinovska, Sonja Kroschwald, Simon Alberti
Protein disorder, prion propensities, and self-organizing macromolecular collectives.
Biochim Biophys Acta, 1834(5) 918-931 (2013)
Sandra Tenreiro, Matthias Munder, Simon Alberti, Tiago Fleming Outeiro
Harnessing the power of yeast to unravel the molecular basis of neurodegeneration.
J Neurochem, 127(4) 438-452 (2013)
Paola Picotti, Mathieu Clément-Ziza, Hugo Y K Lam, David S Campbell, Alexander Schmidt, Eric W Deutsch, Hannes Röst, Zhongwei Sun, Oliver Rinner, Lukas Reiter, Qin Shen, Jacob J Michaelson, Andreas Frei, Simon Alberti, Ulrike Kusebauch, Bernd Wollscheid, Robert L Moritz, Andreas Beyer, Ruedi Aebersold
A complete mass-spectrometric map of the yeast proteome applied to quantitative trait analysis.
Nature, 494(7436) 266-270 (2013)


Simon Alberti
Molecular mechanisms of spatial protein quality control.
Prion, 6(5) 437-442 (2012)
Randal Halfmann, Jessica Wright, Simon Alberti, Susan Lindquist, Michael Rexach
Prion formation by a yeast GLFG nucleoporin.
Prion, 6(4) 1-9 (2012)
Liliana Malinovska, Sonja Kroschwald, Matthias Munder, Doris Richter, Simon Alberti
Molecular chaperones and stress-inducible protein-sorting factors coordinate the spatiotemporal distribution of protein aggregates
Mol Biol Cell, 23(16) 3041-3056 (2012)


Randal Halfmann, Simon Alberti, Rajaraman Krishnan, Nicholas Lyle, Charles W O'Donnell, Oliver D King, Bonnie Berger, Rohit V Pappu, Susan Lindquist
Opposing effects of glutamine and asparagine govern prion formation by intrinsically disordered proteins.
Mol Cell, 43(1) 72-84 (2011)


Randal Halfmann, Simon Alberti, Susan Lindquist
Prions, protein homeostasis, and phenotypic diversity.
Trends Cell Biol, 20(3) 125-133 (2010)
Simon Alberti, Randal Halfmann, Susan Lindquist
Biochemical, cell biological, and genetic assays to analyze amyloid and prion aggregation in yeast.
Meth Enzymol, 470 709-734 (2010)


Simon Alberti, Randal Halfmann, Oliver King, Atul Kapila, Susan Lindquist
A systematic survey identifies prions and illuminates sequence features of prionogenic proteins.
Cell, 137(1) 146-158 (2009)


Simon Alberti, Aaron D Gitler, Susan Lindquist
A suite of Gateway cloning vectors for high-throughput genetic analysis in Saccharomyces cerevisiae.
Yeast, 24(10) 913-919 (2007)


Gerlinde Grelle, Susanne Kostka, Albrecht Otto, Birgit Kersten, Klaus F Genser, Eva-Christina Müller, Stephanie Wälter, Annett Böddrich, Ulrich Stelzl, Christian Hänig, Rudolf Volkmer-Engert, Christiane Landgraf, Simon Alberti, Jörg Höhfeld, Martin Strödicke, Erich E Wanker
Identification of VCP/p97, carboxyl terminus of Hsp70-interacting protein (CHIP), and amphiphysin II interaction partners using membrane-based human proteome arrays.
Mol Cell Proteomics, 5(2) 234-244 (2006)


Verena Arndt, Christina Daniel, Wolfgang Nastainczyk, Simon Alberti, Jörg Höhfeld
BAG-2 acts as an inhibitor of the chaperone-associated ubiquitin ligase CHIP.
Mol Biol Cell, 16(12) 5891-5900 (2005)


Claudia Esser, Simon Alberti, Jörg Höhfeld
Cooperation of molecular chaperones with the ubiquitin/proteasome system.
Biochim Biophys Acta, 1695(1-3) 171-188 (2004)
Simon Alberti, Karsten Böhse, Verena Arndt, Anton Schmitz, Jörg Höhfeld
The cochaperone HspBP1 inhibits the CHIP ubiquitin ligase and stimulates the maturation of the cystic fibrosis transmembrane conductance regulator.
Mol Biol Cell, 15(9) 4003-4010 (2004)


Simon Alberti, Claudia Esser, Jörg Höhfeld
BAG-1--a nucleotide exchange factor of Hsc70 with multiple cellular functions.
Cell Stress Chaperones, 8(3) 225-231 (2003)


Simon Alberti, Jens Demand, Claudia Esser, Niels Emmerich, Hansjorg Schild, Jorg Hohfeld
Ubiquitylation of BAG-1 suggests a novel regulatory mechanism during the sorting of chaperone substrates to the proteasome.
J Biol Chem, 277(48) 45920-45927 (2002)


Jens Demand, Simon Alberti, C Patterson, Jörg Höhfeld
Cooperation of a ubiquitin domain protein and an E3 ubiquitin ligase during chaperone/proteasome coupling.
Curr Biol, 11(20) 1569-1577 (2001)