Welcome to the esiRNA web-page of the Max Planck Institute of Molecular Cell Biology and Genetics. RNAi is a powerful tool for gene function studies and different silencing triggers have been developed to elicit an RNAi response in a variety of systems. esiRNAs are enzymatically prepared siRNAs, resulting from digestion of long double stranded RNAs with an RNaseIII family enzyme in vitro. This page contains information about the production and performance of esiRNAs that should help users to perform successful RNAi experiments with this reagent.

Time-laps movie of HeLa cells transfected with esiRNA targeting firefly luciferase (LUC) and a protein of the anaphase promoting complex (CDC16), respectively. Movies were started 24 hours post transfection and frames were recorded every 10 minutes. Note the increase of cells arrested in mitosis at later stages in the CDC16 knockdown.