esiRNAs can be produced in high throughput at high quality. To assist the design of quality optimized esiRNAs, the algorithm Deqor can help to find the best region for esiRNA synthesis in any given transcript. Pre-designed optimized esiRNAs for human, mouse and rat can be found in the database RiDDLE. Ready to use Genome-scale esiRNA libraries are also available from Sigma-Aldrich.

A genome scale human esiRNA library arrayed in 384 well plates is shown.