Services & Facilities

The Transgenic Core Facility (TCF) provides a centralized resource and state-of-the-art technology for the production of knock-out mice by injection or aggregation of embryonic stem cells into mouse embryos, and transgenic mice by injection of DNA into the pronucleus of mouse oocytes.

Services include:

  • Blastocyst injection / Morula laser injection
  • CRISPR and Pronuclear injection (plasmids and BACs)
  • Cleaning and import by embryo transfers
  • In vitro fertilization
  • Backup freezing of sperm or embryos
  • Electroporation of DNA constructs into mouse ES-Cells
  • Deriving of new mouse ES-cell lines