Research Groups

GAIA PIGINO
GROUP

Electron Microscopy of Resin Embedded Samples

Cross section of Tetrahymena cilia from the oral apparatus.
Cross section of human trachea cilia.
Cross section of Tetrahymena cilia.

Single Particle Analysis

Negatively stained yeast proteins
that tend to form filaments.
3D model reconstituted in EMAN2.

TIRF Microscopy

CLEM

(Correlative Light and Electron Microscopy)

Cross sections of microtubule doublets with attached IFT trains are averaged. The directions of the trains were previously detected by TIRF microscopy. Thus, we could show that anterograde trains (B, green in D) travel along the B-tubule, while retrograde trains (C, magenta in D) only travel along A-tubules. In this way the trains avoid collision.

Immuno Fluorescence

Immuno fluorescence staining of primary cilia from MDCK cells
(blue: DAPI, red: acetylated tubulin,green: Arl13b – ciliary membrane)
Confocal immuno fluorescence
of Tetrahymena cells.