Research Groups

ALF HONIGMANN
GROUP

Membrane Organization of Cells and Tissues

Biological membranes are fascinating composites of lipids, proteins and sugars. Our aim is to understand how the molecular components self-organize into meso-scale complexes that control processes as adhesion, polarization, signaling / sensing, which underlie morphogenesis of cells and tissues.

To this end, we pursue a mutli-scale approach:

(1) Supra-Molecular-Organization of Membrane Complexes in Cells and Tissues 
We use and develop super-resolution STED microscopy in combination with 3D tissue culture to study how molecules organize into functional meso-scale structures. Currently we are working on the Apical Junction Complex. 

(2) Reconstitution of Membrane Complexes by Molecular Self-Organization in vitro 
We purify key organizing proteins to re-assemble complex structures and functions in controlled in vitro membrane systems. We are particularly interested in collective processes such as phase transitions / separation and active feedback systems.

(3) Control of Morphogenesis in Epithelial Organoids
We aim to apply and test the molecular and mesoscale self-organization principles during the morphogenesis of stem cell derived organoids. We especially focus on the interplay of cell adhesion, cortical mechanics and cell differentiation.

 

 

Methods

  • Super-Resolution Microscopy (STED, SMLM)
  • Fluorescence Correlation Spectroscopy, Single Molecule Tracking
  • 2D and 3D Tissue Culture of Epithelial and Stem Cells
  • Protein Purification and Biochemistry
  • Reconstitution of Proteins on Model Membranes
  • Genetics (Crispr KI and KO)
  • Micro-Patterning of Surfaces for Control of Cell Adhesion and Polarization