Research Groups

2020

Jordina Guillén-Boixet, Andrii Kopach, Alex S Holehouse, Sina Wittmann, Marcus Jahnel, Raimund Schlüßler, Kyoohyun Kim, Irmela Trussina, Jie Wang, Daniel Mateju, Ina Poser, Shovamayee Maharana, Martine Ruer-Gruß, Doris Richter, Xiaojie Zhang, Young-Tae Chang, Jochen Guck, Alf Honigmann, Julia Mahamid, Anthony Hyman, Rohit V Pappu, Simon Alberti, Titus Franzmann
RNA-Induced Conformational Switching and Clustering of G3BP Drive Stress Granule Assembly by Condensation.
Cell, 181(2) 346-361 (2020)
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Riccardo Maraspini, Chen-Ho Wang, Alf Honigmann
Optimization of 2D and 3D cell culture to study membrane organization with STED microscopy
J Phys D: Appl Phys, 53(1) Art. No. 014001 (2020)
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Adam Klosin, F Oltsch, T Harmon, Alf Honigmann, Frank Jülicher, Anthony A. Hyman, Christoph Zechner
Phase separation provides a mechanism to reduce noise in cells.
Science, 367(6476) 464-468 (2020)
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Alka Bhat, Linjie Lu, Chen-Ho Wang, Simon Lo Vecchio, Riccardo Maraspini, Alf Honigmann, Daniel Riveline
How to orient cells in microcavities for high resolution imaging of cytokinesis and lumen formation.
Methods Cell Biol, 158 25-41 (2020)
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2019

Oliver Beutel, Riccardo Maraspini, Karina Pombo-García, Cécilie Martin-Lemaitre, Alf Honigmann
Phase Separation of Zonula Occludens Proteins Drives Formation of Tight Junctions.
Cell, 179(4) 923-936 (2019)
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Beatrice Senigagliesi, Carlotta Penzo, Luisa Ulloa Severino, Riccardo Maraspini, Sara Petrosino, Hernán Morales-Navarrete, Enrico Pobega, Elena Ambrosetti, Pietro Parisse, Silvia Pegoraro, Guidalberto Manfioletti, Loredana Casalis, Riccardo Sgarra
The High Mobility Group A1 (HMGA1) Chromatin Architectural Factor Modulates Nuclear Stiffness in Breast Cancer Cells.
Int J Mol Sci, 20(11) Art. No. 2733 (2019)
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2018

Edouard Mobarak, Matti Javanainen, Waldemar Kulig, Alf Honigmann, Erdinc Sezgin, Noora Aho, Christian Eggeling, Tomasz Rog, Ilpo Vattulainen
How to minimize dye-induced perturbations while studying biomembrane structure and dynamics: PEG linkers as a rational alternative.
Biochim Biophys Acta Biomembr, 1860(11) 2436-2445 (2018)
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Mengfei Gao, Riccardo Maraspini, Oliver Beutel, Amin Zehtabian, Britta Eickholt, Alf Honigmann, Helge Ewers
Expansion Stimulated Emission Depletion Microscopy (ExSTED).
ACS Nano, 12(5) 4178-4185 (2018)
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Daniëlle R J Verboogen, Martin Ter Beest, Alf Honigmann, Geert van den Bogaart
Secretory vesicles of immune cells contain only a limited number of interleukin 6 molecules.
FEBS Lett, 592(9) 1535-1544 (2018)
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Riccardo Maraspini, Oliver Beutel, Alf Honigmann
Circle scanning STED fluorescence correlation spectroscopy to quantify membrane dynamics and compartmentalization.
Methods, 140-141 188-197 (2018)
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Alf Honigmann, André Nadler
The Next Frontier: Quantitative Biochemistry in Living Cells.
Biochemistry, 57(1) 47-55 (2018)
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2017

Julia P Steringer, Sascha Lange, Sabína Čujová, Radek Šachl, Chetan Poojari, Fabio Lolicato, Oliver Beutel, Hans-Michael Müller, Sebastian Unger, Ünal Coskun, Alf Honigmann, Ilpo Vattulainen, Martin Hof, Christian Freund, Walter Nickel
Key steps in unconventional secretion of fibroblast growth factor 2 reconstituted with purified components.
Elife, 6 Art. No. e28985 (2017)
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Jeffrey Woodruff, Beatriz Ferreira Gomes, Per Widlund, Julia Mahamid, Alf Honigmann, Anthony Hyman
The Centrosome Is a Selective Condensate that Nucleates Microtubules by Concentrating Tubulin.
Cell, 169(6) 1066-1077 (2017)
DOI

2016

Alf Honigmann, A Pralle
Compartmentalization of the Cell Membrane.
J Mol Biol, 428(24 Pt A) 4739-4748 (2016)
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Maksim V Baranov, Natalia H Revelo, Ilse Dingjan, Riccardo Maraspini, Martin Ter Beest, Alf Honigmann, Geert van den Bogaart
SWAP70 Organizes the Actin Cytoskeleton and Is Essential for Phagocytosis.
Cell Rep, 17(6) 1518-1531 (2016)
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Christian Eggeling, Alf Honigmann
Closing the gap: The approach of optical and computational microscopy to uncover biomembrane organization.
Biochim Biophys Acta, 1858(10) 2558-2568 (2016)
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Olena Steshenko, Débora M Andrade, Alf Honigmann, Veronika Mueller, Falk Schneider, Erdinc Sezgin, Stefan W Hell, Mikael Simons, Christian Eggeling
Reorganization of Lipid Diffusion by Myelin Basic Protein as Revealed by STED Nanoscopy.
Biophys J, 110(11) 2441-2450 (2016)
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Dragomir Milovanovic, Mitja Platen, Meike Junius, Ulf Diederichsen, Iwan A T Schaap, Alf Honigmann, Reinhard Jahn, Geert van den Bogaart
Calcium Promotes the Formation of Syntaxin 1 Mesoscale Domains through Phosphatidylinositol 4,5-Bisphosphate.
J Biol Chem, 291(15) 7868-7876 (2016)
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Erdinc Sezgin, Fatma Betul Can, Falk Schneider, Mathias P Clausen, Silvia Galiani, Tess A Stanly, Dominic Waithe, Alexandria Colaco, Alf Honigmann, Daniel Wüstner, Frances Platt, Christian Eggeling
A comparative study on fluorescent cholesterol analogs as versatile cellular reporters.
J Lipid Res, 57(2) 299-309 (2016)
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2015

Stefan W Hell, Steffen J. Sahl, Mark Bates, Xiaowei Zhuang, Rainer Heintzmann, Martin J. Booth, Joerg Bewersdorf, Gleb Shtengel, Harald Hess, Philip Tinnefeld, Alf Honigmann, Stefan Jakobs, Ilaria Testa, Laurent Cognet, Brahim Lounis, Helge Ewers, Simon J. Davis, Christian Eggeling, David Klenerman, Katrin I. Willig, Giuseppe Vicidomini, Marco Castello, Alberto Diaspro, Thorben Cordes
The 2015 super-resolution microscopy roadmap
J Phys D: Appl Phys, 48(44) Art. No. 443001 (2015)
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Giuseppe Vicidomini, Haisen Ta, Alf Honigmann, Veronika Mueller, Mathias P Clausen, Dominic Waithe, Silvia Galiani, Erdinc Sezgin, Alberto Diaspro, Stefan W Hell, Christian Eggeling
STED-FLCS: An Advanced Tool to Reveal Spatiotemporal Heterogeneity of Molecular Membrane Dynamics.
Nano Lett, 15(9) 5912-5918 (2015)
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Dragomir Milovanovic, Alf Honigmann, Seiichi Koike, Fabian Göttfert, Gesa Pähler, Meike Junius, Stefan Müllar, Ulf Diederichsen, Andreas Janshoff, Helmut Grubmüller, H Jelger Risselada, Christian Eggeling, Stefan W Hell, Geert van den Bogaart, Reinhard Jahn
Hydrophobic mismatch sorts SNARE proteins into distinct membrane domains.
Nat Commun, 6 Art. No. 5984 (2015)
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Eva Sevcsik, Mario Brameshuber, Martin Fölser, Julian Weghuber, Alf Honigmann, Gerhard J Schütz
GPI-anchored proteins do not reside in ordered domains in the live cell plasma membrane.
Nat Commun, 6 6969-6969 (2015)
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2014

Alf Honigmann, Veronika Mueller, Haisen Ta, Andreas Schoenle, Erdinc Sezgin, Stefan W Hell, Christian Eggeling
Scanning STED-FCS reveals spatiotemporal heterogeneity of lipid interaction in the plasma membrane of living cells.
Nat Commun, 5 5412-5412 (2014)
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Sinem K Saka, Alf Honigmann, Christian Eggeling, Stefan W Hell, Thorsten Lang, Silvio O Rizzoli
Multi-protein assemblies underlie the mesoscale organization of the plasma membrane.
Nat Commun, 5 4509-4509 (2014)
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Alf Honigmann, Sina Sadeghi, Jan Keller, Stefan W Hell, Christian Eggeling, Richard Vink
A lipid bound actin meshwork organizes liquid phase separation in model membranes.
Elife, 3 1671-1671 (2014)
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2013

Fabian Göttfert, Christian A Wurm, Veronika Mueller, Sebastian Berning, Volker C Cordes, Alf Honigmann, Stefan W Hell
Coaligned dual-channel STED nanoscopy and molecular diffusion analysis at 20 nm resolution.
Biophys J, 105(1) 1-3 (2013)
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Alf Honigmann, Geert van den Bogaart, Emilio Iraheta, H Jelger Risselada, Dragomir Milovanovic, Veronika Mueller, Stefan Müllar, Ulf Diederichsen, Dirk Fasshauer, Helmut Grubmüller, Stefan W Hell, Christian Eggeling, Karin Kühnel, Reinhard Jahn
Phosphatidylinositol 4,5-bisphosphate clusters act as molecular beacons for vesicle recruitment.
Nat Struct Mol Biol, 20(6) 679-686 (2013)
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