Services & Facilities

Long read DNA sequencing and associated technologies

We are one of three platforms of the DRESDEN-concept Genome Center (a cooperation between the TU Dresden and the MPI-CBG), and as such we are applying different long read sequencing, haplotyping, and scaffolding technologies to improve de novo whole genome assemblies. We are also offering additional long read applications, for example sequencing of long RNA isoforms and barcoded amplicon sequencing.

The DRESDEN-concept Genome Center is a collaboration between the Deep Sequencing Laboratory of the TUD and the Sequencing and Genotyping Facility of the MPI-CBG and offers a broad range of state-of-the-art genomics technologies.

In March 2018, the DGC became awarded as one of four DFG funded NGS competence centers in Germany.
Link to the Press Release

Special expertise:
The long-read DNA sequencing platform is closely collaborating with Gene Myers on the area of high-quality genome assemblies and is actively participating in several synergistic sequencing projects such as the Vertebrate Genomes Project (VGP), EuroFish, and Bat1K project. We focus specifically on the extraction of ultralong genomic DNA that is suitable for our long read technologies.

Press releases of the VGP and Bat1K projects:
22.07.2020 The genetic basis of bats’ superpowers revealed
13.09.2018 International Vertebrate Genomes Project releases first 15 new genomes

Services

  • Pacific Bioscience Single Molecule Real Time (SMRT) Sequencing 

    • of ultralong genomic DNA
    • of RNA isoforms
    • of PCR amplicons

  • Genome Sequencing making use of 10X Genomics read clouds for haplotype-phasing and structural variant detection
  • Whole genome confirmation capture for genome scaffolding (HiC protocol)
  • Bionano optical mapping
  • Extraction of HMW genomic DNA from cells and tissues
  • First entry point for NGS-based applications making use of second and third generation sequencing technologies

Membership in professional associations

Core4Life – association of biomolecular research facilities