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Anthony Hyman

Microtubules and cell division

We are generally interested in spatial control of the microtubule cytoskeleton, and how this applies to the events necessary for mitosis and cell division. In particular we are interested in problems of size and shape.  What sets the length of a mitotic spindle?  Why is a spindle precisely positioned in a cell?  Why do two cortical domains form of a certain size?  Two technical steps forward have revolutionized the study of these problems in the last ten years.  One is the development of genomics techniques, including RNA interference, and BAC transgenesis.  The other is the contribution of physics to understanding biology. In our lab, and in collaboration with the labs of Joe Howard, Stephan Grill and Frank Buchholz at the CBG, we combine these approaches to study the following

a) Mitotic spindle assembly and function, focusing on centrosomes.
b) Distribution of force generating mechanisms necessary for the first asymmetric division.
c) Establishment of cortical polarity.

We primarily work in C.elegans embryos, but we are also studying aspects of these problems in Human cells, using the emerging techniques of BAC transgenesis.

For further information also visit our lab-homepage at http://hymanlab.mpi-cbg.de/hyman_lab/.

Selected Publications

2013
Reber, Simone; Baumgart, Johannes; Widlund, Per; Pozniakovsky, Andrei I.; Howard, Jonathon; Hyman, Anthony; Jülicher, Frank
XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules
Nat. Cell Biol., 15, no. 9, pp. 1116-1122, (2013)
2012
Sarov, Mihail; Murray, John I; Schanze, Kristin; Pozniakovski, Andrei; Niu, Wei; Angermann, Karolin; Hasse, Susanne; Rupprecht, Michaela; Vinis, Elisabeth; Tinney, Matthew; Preston, Elicia A.; Zinke, Andrea; Enst, Susanne; Teichgraber, Tina; Janette, Judith; Reis, Kadri; Janosch, Stephan; Schloissnig, Siegfried; Ejsmont, Radoslaw K; Slightam, Cindie; Xu, Xiao; Kim, Stuart K; Reinke, Valerie; Stewart, A Francis; Snyder, Michael; Waterston, Robert H; Hyman, Anthony A.
A Genome-Scale Resource for In Vivo Tag-Based Protein Function Exploration in C. elegans.
Cell, 150, no. 4, pp. 855-866, (2012)
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Wurzenberger, Claudia; Held, Michael; Lampson, Michael A; Poser, Ina; Hyman, Anthony A.; Gerlich, Daniel W
Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores.
J. Cell Biol., 198, no. 2, pp. 173-183, (2012)
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2011
Hyman, Anthony A.
Whither systems biology.
Philos. Trans. R. Soc. Lond., B, Biol. Sci., 366, no. 1584, pp. 3635-3637, (2011)
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Reber, Simone; Hyman, Anthony A.
Samurai sword sets spindle size.
Cell, 147, no. 6, pp. 1224-1225, (2011)
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Hyman, Anthony A.; Simons, Kai
Beyond HeLa cells
Nature, 480, no. 7375, pp. 34-34, (2011)
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Hegemann, Björn; Hutchins, James R A; Hudecz, Otto; Novatchkova, Maria; Rameseder, Jonathan; Sykora, Martina M; Liu, Shang-Yun; Mazanek, Michael; Lénárt, Péter; Hériché, Jean-Karim; Poser, Ina; Kraut, Norbert; Hyman, Anthony A.; Yaffe, Michael B; Mechtler, Karl; Peters, Jan-Michael
Systematic phosphorylation analysis of human mitotic protein complexes.
Sci. Signal., 4, no. 198, (2011)
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Elefsinioti, Antigoni; Saraç, Ömer Sinan; Hegele, Anna; Plake, Conrad; Hubner, Nina C; Poser, Ina; Sarov, Mihail; Hyman, Anthony A.; Mann, Matthias; Schroeder, Michael; Stelzl, Ulrich; Beyer, Andreas
Large-scale de novo prediction of physical protein-protein association.
Mol. Cell Proteomics, 10, no. 11, (2011)
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Decker, Markus; Jaensch, Steffen; Pozniakovsky, Andrei I.; Zinke, Andrea; O'Connell, Kevin F; Zachariae, Wolfgang; Myers, Eugene; Hyman, Anthony A.
Limiting amounts of centrosome material set centrosome size in C. elegans embryos.
Curr. Biol., 21, no. 15, pp. 1259-1267, (2011)
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Stewart, Martin P; Toyoda, Yusuke; Hyman, Anthony A.; Müller, Daniel J.
Force probing cell shape changes to molecular resolution.
Trends Biochem. Sci., 36, no. 8, pp. 444-450, (2011)
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Hyman, Anthony A.; Brangwynne, Clifford
Beyond stereospecificity: liquids and mesoscale organization of cytoplasm.
Dev. Cell, 21, no. 1, pp. 14-16, (2011)
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Green, Rebecca A; Kao, Huey-Ling; Audhya, Anjon; Arur, Swathi; Mayers, Jonathan R; Fridolfsson, Heidi N; Schulman, Monty; Schloissnig, Siegfried; Niessen, Sherry; Laband, Kimberley; Wang, Shaohe; Starr, Daniel A; Hyman, Anthony A.; Schedl, Tim; Desai, Arshad; Piano, Fabio; Gunsalus, Kristin C.; Oegema, Karen
A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue.
Cell, 145, no. 3, pp. 470-482, (2011)
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Jakobsen, Lis; Vanselow, Katja; Skogs, Marie; Toyoda, Yusuke; Lundberg, Emma; Poser, Ina; Falkenby, Lasse G; Bennetzen, Martin; Westendorf, Jens; Nigg, Erich A; Uhlen, Mathias; Hyman, Anthony A.; Andersen, Jens S
Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
EMBO J., 30, no. 8, pp. 1520-1535, (2011)
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Brangwynne, Clifford; Mitchison, Timothy J.; Hyman, Anthony A.
Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes.
Proc. Natl. Acad. Sci. U.S.A., 108, no. 11, pp. 4334-4339, (2011)
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Guizetti, Julien; Schermelleh, Lothar; Mäntler, Jana; Maar, Sandra; Poser, Ina; Leonhardt, Heinrich; Müller-Reichert, Thomas; Gerlich, Daniel W
Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments.
Science, 331, no. 6024, pp. 1616-1620, (2011)
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Schnütgen, Frank; Ehrmann, Franziska; Poser, Ina; Hubner, Nina C; Hansen, Jens; Floss, Thomas; deVries, Ingrid; Wurst, Wolfgang; Hyman, Anthony A.; Mann, Matthias; Melchner, Harald von
Resources for proteomics in mouse embryonic stem cells.
Nat. Methods, 8, no. 2, pp. 103-104, (2011)
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Behnke-Parks, William M; Vendome, Jeremie; Honig, Barry; Maliga, Zoltan; Moores, Carolyn; Rosenfeld, Steven S
Loop L5 acts as a conformational latch in the mitotic kinesin Eg5.
J. Biol. Chem., 286, no. 7, pp. 5242-5253, (2011)
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Begasse, Maria L; Hyman, Anthony A.
The first cell cycle of the Caenorhabditis elegans embryo: spatial and temporal control of an asymmetric cell division.
Cell Cycle in Development, chapt. 6, pp. 109-133, in: Results and Problems in Cell Differentiation, 53, (2011)
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Gell, Christopher; Friel, Claire; Borgonovo, Barbara; Drechsel, David N.; Hyman, Anthony A.; Howard, Jonathon
Purification of tubulin from porcine brain.
Methods Mol. Biol., 777, pp. 15-28, (2011)
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Ettinger, Andreas; Wilsch-Bräuninger, Michaela; Marzesco, Anne-Marie; Bickle, Marc; Lohmann, Annett; Maliga, Zoltan; Karbanová, Jana; Corbeil, Denis; Hyman, Anthony A.; Huttner, Wieland B.
Proliferating versus differentiating stem and cancer cells exhibit distinct midbody-release behaviour.
Nat. Commun., 2, (2011)
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2008
Boxem, Mike; Maliga, Zoltan; Klitgord, Niels; Li, Na; Lemmens, Irma; Mana, Miyeko; Lichtervelde, Lorenzo de; Mul, Joram D.; Peut, Diederik van de; Devos, Maxime; Simonis, Nicolas; Yildirim, Muhammed A.; Cokol, Murat; Kao, Huey-Ling; Smet, Anne-Sophie de; Schlaitz, Anne-Lore; Wang, Haidong; Hao, Tong; Milstein, Stuart; Fan, Changyu; Tipsword, Mike; Drew, Kevin; Galli, Matilde; Rhrissorrakrai, Kahn; Drechsel, David N.; Koller, Daphne; Roth, Frederick P.; Iakoucheva, Lilia M.; Dunker, A. Keith; Bonneau, Richard; Gunsalus, Kristin C.; Hill, David E.; Piano, Fabio; Tavernier, Jan; Heuvel, Sander van den; Hyman, Anthony A.; Vidal, Marc
A protein domain-based interactome network for C. elegans early embryogenesis.
Cell, 134, no. 3, pp. 534-545, (2008)
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Poser, Ina; Sarov, Mihail; Hutchins, James R A; Hériché, Jean-Karim; Toyoda, Yusuke; Pozniakovsky, Andrei I.; Weigl, Daniela; Nitzsche, Anja; Hegemann, Björn; Bird, Alexander W.; Pelletier, Laurence; Kittler, Ralf; Hua, Sujun; Naumann, Ronald; Augsburg, Martina; Sykora, Martina M; Hofemeister, Helmut; Zhang, Youming; Nasmyth, Kim; White, Kevin P; Dietzel, Steffen; Mechtler, Karl; Durbin, Richard; Stewart, A. Francis; Peters, Jan-Michael; Buchholz, Frank; Hyman, Anthony A.
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.
Nat. Methods, 5, no. 5, pp. 409-415, (2008)
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Brouhard, Gary J.; Stear, Jeffrey H.; Noetzel, Tim L.; Al-Bassam, Jawdat; Kinoshita, Kazuhisa; Harrison, Stephen C.; Howard, Jonathon; Hyman, Anthony A.
XMAP215 is a processive microtubule polymerase.
Cell, 132, no. 1, pp. 79-88, (2008)
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2006
Pecreaux, Jacques; Röper, Jens-Christian; Kruse, Karsten; Jülicher, Frank; Hyman, Anthony A.; Grill, Stephan W.; Howard, Jonathon
Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators.
Curr. Biol., 16, no. 21, pp. 2111-2122, (2006)
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Pelletier, Laurence; O'Toole, Eileen T.; Schwager, Anne; Hyman, Anthony A.; Müller-Reichert, Thomas
Centriole assembly in Caenorhabditis elegans.
Nature, 444, no. 7119, pp. 619-623, (2006)
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2005
Bringmann, Henrik; Hyman, Anthony A.
A cytokinesis furrow is positioned by two consecutive signals.
Nature, 436, no. 7051, pp. 731-734, (2005)
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