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Anthony Hyman

Organization of Cytoplasm

My group is interested in organization, size, and shape of non-membrane compartments in cells. We work on this problem using a number of different examples, including:

  • Centrosomes, which are the major sites of microtubule nucleation
  • The cortex, which is a major signaling center to organize cells
  • P granules, which are collections of RNA/proteins required for the formation of the germline.

We primarily use C.elegans as a model system, but we complement this with studies in Xenopus extracts and human cells. Our work, using genome-wide RNAi screens, has shown that rather small numbers of molecular components are required to organize each of these compartments. Using a combination of genetics and physics, we have discovered that a principle underlying the organization of these structures is liquid-liquid phase separation. We study these compartments both in vivo and in vitro, using reconstitution methods.

We complement our studies by taking genomics approaches to cataloguing the proteins required for forming these compartments. We have followed up on our RNAi screens by developing methods to tag proteins in large pieces of genomic DNA, using recombineering. This has allowed us to make genome-wide libraries of tagged proteins for follow up of genes identified by screening.

For further information also visit our lab-homepage at http://hymanlab.mpi-cbg.de/hyman_lab/.

Selected Publications

2015
Begasse, Maria L; Leaver, Mark; Vazquez, Federico; Grill, Stephan W.; Hyman, Anthony
Temperature Dependence of Cell Division Timing Accounts for a Shift in the Thermal Limits of C. elegans and C. briggsae.
Cell Rep, 10, no. 5, pp. 647-653, (2015)
2014
Wueseke, Oliver; Bunkenborg, Jakob; Hein, Marco Y; Zinke, Andrea; Viscardi, Valeria; Woodruff, Jeffrey; Oegema, Karen; Mann, Matthias; Andersen, Jens S; Hyman, Anthony
The Caenorhabditis elegans pericentriolar material components SPD-2 and SPD-5 are monomeric in the cytoplasm before incorporation into the PCM matrix.
Mol Biol Cell, 25, no. 19, pp. 2984-2992, (2014)
Woodruff, Jeffrey; Wueseke, Oliver; Hyman, Anthony
Pericentriolar material structure and dynamics.
Philos. Trans. R. Soc. Lond., B, Biol. Sci., 369, no. 1650, (2014)
Toyoda, Yusuke; Erkut, Cihan; Pan-Montojo, Francisco; Boland, Sebastian; Stewart, Martin P; Müller, Daniel J. ; Wurst, Wolfgang; Hyman, Anthony; Kurzchalia, Teymuras V.
Products of the Parkinson's disease-related glyoxalase DJ-1, D-lactate and glycolate, support mitochondrial membrane potential and neuronal survival.
Biol Open, 3, no. 8, pp. 777-784, (2014)
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Zwicker, David; Decker, Markus; Jaensch, Steffen; Hyman, Anthony; Jülicher, Frank
Centrosomes are autocatalytic droplets of pericentriolar material organized by centrioles.
Proc. Natl. Acad. Sci. U.S.A., 111, no. 26, pp. 2636-2645, (2014)
Hyman, Anthony
Encouraging innovation.
Mol Biol Cell, 25, no. 4, pp. 427-428, (2014)
Hyman, Anthony; Weber, Christian; Jülicher, Frank
Liquid-liquid phase separation in biology.
Annu Rev Cell Dev Biol, 30, pp. 39-58, (2014)
2013
Reber, Simone; Baumgart, Johannes; Widlund, Per; Pozniakovsky, Andrei I.; Howard, Jonathon; Hyman, Anthony; Jülicher, Frank
XMAP215 activity sets spindle length by controlling the total mass of spindle microtubules
Nat Cell Biol, 15, no. 9, pp. 1116-1122, (2013)
2012
Sarov, Mihail; Murray, John I; Schanze, Kristin; Pozniakovski, Andrei; Niu, Wei; Angermann, Karolin; Hasse, Susanne; Rupprecht, Michaela; Vinis, Elisabeth; Tinney, Matthew; Preston, Elicia A.; Zinke, Andrea; Enst, Susanne; Teichgraber, Tina; Janette, Judith; Reis, Kadri; Janosch, Stephan; Schloissnig, Siegfried; Ejsmont, Radoslaw K; Slightam, Cindie; Xu, Xiao; Kim, Stuart K; Reinke, Valerie; Stewart, A Francis; Snyder, Michael; Waterston, Robert H; Hyman, Anthony A.
A Genome-Scale Resource for In Vivo Tag-Based Protein Function Exploration in C. elegans.
Cell, 150, no. 4, pp. 855-866, (2012)
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Wurzenberger, Claudia; Held, Michael; Lampson, Michael A; Poser, Ina; Hyman, Anthony A.; Gerlich, Daniel W
Sds22 and Repo-Man stabilize chromosome segregation by counteracting Aurora B on anaphase kinetochores.
J. Cell Biol., 198, no. 2, pp. 173-183, (2012)
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2011
Hyman, Anthony A.
Whither systems biology.
Philos. Trans. R. Soc. Lond., B, Biol. Sci., 366, no. 1584, pp. 3635-3637, (2011)
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Reber, Simone; Hyman, Anthony A.
Samurai sword sets spindle size.
Cell, 147, no. 6, pp. 1224-1225, (2011)
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Hyman, Anthony A.; Simons, Kai
Beyond HeLa cells
Nature, 480, no. 7375, pp. 34-34, (2011)
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Hegemann, Björn; Hutchins, James R A; Hudecz, Otto; Novatchkova, Maria; Rameseder, Jonathan; Sykora, Martina M; Liu, Shang-Yun; Mazanek, Michael; Lénárt, Péter; Hériché, Jean-Karim; Poser, Ina; Kraut, Norbert; Hyman, Anthony A.; Yaffe, Michael B; Mechtler, Karl; Peters, Jan-Michael
Systematic phosphorylation analysis of human mitotic protein complexes.
Sci. Signal., 4, no. 198, (2011)
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Elefsinioti, Antigoni; Saraç, Ömer Sinan; Hegele, Anna; Plake, Conrad; Hubner, Nina C; Poser, Ina; Sarov, Mihail; Hyman, Anthony A.; Mann, Matthias; Schroeder, Michael; Stelzl, Ulrich; Beyer, Andreas
Large-scale de novo prediction of physical protein-protein association.
Mol Cell Proteomics, 10, no. 11, (2011)
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Decker, Markus; Jaensch, Steffen; Pozniakovsky, Andrei I.; Zinke, Andrea; O'Connell, Kevin F; Zachariae, Wolfgang; Myers, Eugene; Hyman, Anthony A.
Limiting amounts of centrosome material set centrosome size in C. elegans embryos.
Curr Biol, 21, no. 15, pp. 1259-1267, (2011)
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Stewart, Martin P; Toyoda, Yusuke; Hyman, Anthony A.; Müller, Daniel J.
Force probing cell shape changes to molecular resolution.
Trends Biochem. Sci., 36, no. 8, pp. 444-450, (2011)
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Hyman, Anthony A.; Brangwynne, Clifford
Beyond stereospecificity: liquids and mesoscale organization of cytoplasm.
Dev Cell, 21, no. 1, pp. 14-16, (2011)
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Green, Rebecca A; Kao, Huey-Ling; Audhya, Anjon; Arur, Swathi; Mayers, Jonathan R; Fridolfsson, Heidi N; Schulman, Monty; Schloissnig, Siegfried; Niessen, Sherry; Laband, Kimberley; Wang, Shaohe; Starr, Daniel A; Hyman, Anthony A.; Schedl, Tim; Desai, Arshad; Piano, Fabio; Gunsalus, Kristin C.; Oegema, Karen
A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue.
Cell, 145, no. 3, pp. 470-482, (2011)
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Jakobsen, Lis; Vanselow, Katja; Skogs, Marie; Toyoda, Yusuke; Lundberg, Emma; Poser, Ina; Falkenby, Lasse G; Bennetzen, Martin; Westendorf, Jens; Nigg, Erich A; Uhlen, Mathias; Hyman, Anthony A.; Andersen, Jens S
Novel asymmetrically localizing components of human centrosomes identified by complementary proteomics methods.
EMBO J., 30, no. 8, pp. 1520-1535, (2011)
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Brangwynne, Clifford; Mitchison, Timothy J.; Hyman, Anthony A.
Active liquid-like behavior of nucleoli determines their size and shape in Xenopus laevis oocytes.
Proc. Natl. Acad. Sci. U.S.A., 108, no. 11, pp. 4334-4339, (2011)
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Guizetti, Julien; Schermelleh, Lothar; Mäntler, Jana; Maar, Sandra; Poser, Ina; Leonhardt, Heinrich; Müller-Reichert, Thomas; Gerlich, Daniel W
Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments.
Science, 331, no. 6024, pp. 1616-1620, (2011)
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Schnütgen, Frank; Ehrmann, Franziska; Poser, Ina; Hubner, Nina C; Hansen, Jens; Floss, Thomas; deVries, Ingrid; Wurst, Wolfgang; Hyman, Anthony A.; Mann, Matthias; Melchner, Harald von
Resources for proteomics in mouse embryonic stem cells.
Nat. Methods, 8, no. 2, pp. 103-104, (2011)
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Behnke-Parks, William M; Vendome, Jeremie; Honig, Barry; Maliga, Zoltan; Moores, Carolyn; Rosenfeld, Steven S
Loop L5 acts as a conformational latch in the mitotic kinesin Eg5.
J. Biol. Chem., 286, no. 7, pp. 5242-5253, (2011)
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Begasse, Maria L; Hyman, Anthony A.
The first cell cycle of the Caenorhabditis elegans embryo: spatial and temporal control of an asymmetric cell division.
Cell Cycle in Development, chapt. 6, pp. 109-133, in: Results and Problems in Cell Differentiation, 53, (2011)
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Gell, Christopher; Friel, Claire; Borgonovo, Barbara; Drechsel, David N.; Hyman, Anthony A.; Howard, Jonathon
Purification of tubulin from porcine brain.
Methods Mol Biol, 777, pp. 15-28, (2011)
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Ettinger, Andreas; Wilsch-Bräuninger, Michaela; Marzesco, Anne-Marie; Bickle, Marc; Lohmann, Annett; Maliga, Zoltan; Karbanová, Jana; Corbeil, Denis; Hyman, Anthony A.; Huttner, Wieland B.
Proliferating versus differentiating stem and cancer cells exhibit distinct midbody-release behaviour.
Nat Commun, 2, (2011)
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2008
Boxem, Mike; Maliga, Zoltan; Klitgord, Niels; Li, Na; Lemmens, Irma; Mana, Miyeko; Lichtervelde, Lorenzo de; Mul, Joram D.; Peut, Diederik van de; Devos, Maxime; Simonis, Nicolas; Yildirim, Muhammed A.; Cokol, Murat; Kao, Huey-Ling; Smet, Anne-Sophie de; Schlaitz, Anne-Lore; Wang, Haidong; Hao, Tong; Milstein, Stuart; Fan, Changyu; Tipsword, Mike; Drew, Kevin; Galli, Matilde; Rhrissorrakrai, Kahn; Drechsel, David N.; Koller, Daphne; Roth, Frederick P.; Iakoucheva, Lilia M.; Dunker, A. Keith; Bonneau, Richard; Gunsalus, Kristin C.; Hill, David E.; Piano, Fabio; Tavernier, Jan; Heuvel, Sander van den; Hyman, Anthony A.; Vidal, Marc
A protein domain-based interactome network for C. elegans early embryogenesis.
Cell, 134, no. 3, pp. 534-545, (2008)
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Poser, Ina; Sarov, Mihail; Hutchins, James R A; Hériché, Jean-Karim; Toyoda, Yusuke; Pozniakovsky, Andrei I.; Weigl, Daniela; Nitzsche, Anja; Hegemann, Björn; Bird, Alexander W.; Pelletier, Laurence; Kittler, Ralf; Hua, Sujun; Naumann, Ronald; Augsburg, Martina; Sykora, Martina M; Hofemeister, Helmut; Zhang, Youming; Nasmyth, Kim; White, Kevin P; Dietzel, Steffen; Mechtler, Karl; Durbin, Richard; Stewart, A. Francis; Peters, Jan-Michael; Buchholz, Frank; Hyman, Anthony A.
BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals.
Nat. Methods, 5, no. 5, pp. 409-415, (2008)
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Brouhard, Gary J.; Stear, Jeffrey H.; Noetzel, Tim L.; Al-Bassam, Jawdat; Kinoshita, Kazuhisa; Harrison, Stephen C.; Howard, Jonathon; Hyman, Anthony A.
XMAP215 is a processive microtubule polymerase.
Cell, 132, no. 1, pp. 79-88, (2008)
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2006
Pecreaux, Jacques; Röper, Jens-Christian; Kruse, Karsten; Jülicher, Frank; Hyman, Anthony A.; Grill, Stephan W.; Howard, Jonathon
Spindle oscillations during asymmetric cell division require a threshold number of active cortical force generators.
Curr Biol, 16, no. 21, pp. 2111-2122, (2006)
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Pelletier, Laurence; O'Toole, Eileen T.; Schwager, Anne; Hyman, Anthony A.; Müller-Reichert, Thomas
Centriole assembly in Caenorhabditis elegans.
Nature, 444, no. 7119, pp. 619-623, (2006)
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2005
Bringmann, Henrik; Hyman, Anthony A.
A cytokinesis furrow is positioned by two consecutive signals.
Nature, 436, no. 7051, pp. 731-734, (2005)
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