Some mutant mice may prove to be infertile through an inability to mate or rear a litter. In such a case, it will be necessary to perform an in vitro fertilization to continue the line in a population. The technique involves superovulating females and fertilizing the eggs with fresh or frozen sperm of males. IVF eggs are then transferred as developed two-cell embryos to a 0.5-d pc pseudopregnant recipient foster.

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