Mass Spectrometry

The Mass Spectrometry facility supports proteomic efforts providing identification and characterisation of individual proteins.

Services include:

Identification of gel separated proteins in any protein, EST and genomic databases
Shot-gun analysis for small- and medium-scale proteomic screens
Relative quantification of proteins
Analysis of selected protein modification
Determination of the molecular weight for intact peptides, proteins and small molecules
Gel service: maintenance of 1- and 2-D equipment, protocols, advice
Consulting in planning of proteomic experiments
Upon request: basic analysis of lipids

Equipment:

The equipment is shared between the Mass Spectrometry Facility and the Research Group of Andrej Shevchenko and includes:

LTQ linear ion trap, LTQ Orbitrap and LTQ Velos Orbitrap hybrid mass spectrometers (ThermoScientific Corp., San Jose CA)
Quadrupole time-of-flight mass spectrometer QSTAR Pulsar I (MDS Sciex, Toronto, Canada)
TSQ Vantage (ThermoScientific Corp., San Jose CA)
The LTQ and LTQ Orbitrap machines are equipped with nanoLC systems Ultimate and Ultimate 3000 (Dionex Corp, Sunnyvale CA) and NanoLC-2D System (Eksigent Technologies, Dublin, CA)
The QSTAR Pulsar and LTQ Orbitrap mass spectrometers are interfaced to an automated nanoelectrospray ion sources NanoMate HD and TriVersa (Advion BioSciences, Ithaca NJ)

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Mass Spectrometry services contact Mass Spectrometry The Mass Spectrometry facility supports proteomic efforts providing identification and characterisation of individual proteins. Services include: Identification of gel separated proteins in any protein, EST and genomic databases Shot-gun analysis for small- and medium-scale proteomic screens Relative quantification of proteins Analysis of selected protein modification Determination of the molecular weight for intact peptides, proteins and small molecules Gel service: maintenance of 1- and 2-D equipment, protocols, advice Consulting in planning of proteomic experiments Upon request: basic analysis of lipids Equipment: The equipment is shared between the Mass Spectrometry Facility and the Research Group of Andrej Shevchenko and includes: LTQ linear ion trap, LTQ Orbitrap and LTQ Velos Orbitrap hybrid mass spectrometers (ThermoScientific Corp., San Jose CA) Quadrupole time-of-flight mass spectrometer QSTAR Pulsar I (MDS Sciex, Toronto, Canada) TSQ Vantage (ThermoScientific Corp., San Jose CA) The LTQ and LTQ Orbitrap machines are equipped with nanoLC systems Ultimate and Ultimate 3000 (Dionex Corp, Sunnyvale CA) and NanoLC-2D System (Eksigent Technologies, Dublin, CA) The QSTAR Pulsar and LTQ Orbitrap mass spectrometers are interfaced to an automated nanoelectrospray ion sources NanoMate HD and TriVersa (Advion BioSciences, Ithaca NJ)
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