3d rendering of Drosophila embryo stained with nuclear marker and anti-GFP antibody revealing the expression of FlyFos-CG4702-GFP gene expression reporter. The embryo was imaged with SPIM from 8 angles and reconstructed with bead-based registration framework followed by content-based image fusion. 3d rendering was done Daniel James White using BioImageXD.
Progress of global optimization during bead-based registration of 18-view tiled acquisition of Drosophila blastoderm stage embryo using a spinning disc confocal.
Visualization of registration of 6x6 EM tile system using global optimization of SIFT feature displacement. Green dots are corresponding SIFT features and red lines show the residual error at a given iteration of the optimizer.
Visualization of registration of 3 serial TEM sections consisting of 4x4 image tiles using global optimization of SIFT feature displacement. Green dots are corresponding SIFT features and red lines show the residual error at a given iteration of the optimizer.