Somal translocation of retinal ganglion cells visualized by the Ath5::membraneGFP transgene. Captured by the Lightsheet Z.1 microscope. Movie by Jaroslav Icha, edited by Julia Eichhorn.
Early cleavage divisions of a zebrafish embryo, where mitotic spindles are visualized by the Eef1a1::dclk2-GFP transgene. Movie captured by Jaroslav Icha and Franziska Decker during the PhD course. Imaged at the Lightsheet Z.1 microscope.
An animated z-stack of the retinal pseudotratified neuroepithelium at ~30 hpf, with plasma membranes in green, all nuclei in blue and mitotic nuclei in magenta. Note the apical-most position of all the mitotic nuclei, a result of the inter kinetic nuclear migration in G2.
A division of retinal neuroepithelial cell where the primary cillium is labeled by Arl13b-GFP (magenta) and the microtubule plus tips visualized by EB3-mKate2 (green).
A division of retinal neuroepithelial cell with a horizontal cleavage plane. Nuclei are labeled by H2B-RFP (green) and the apical domain is labeled by Par3-GFP (magenta).
A multiview acquired during the EMBO course in Lightsheet microscopy with Yong Wang, University of Toronto. The movie shows a transgenic line Ptf1a:GFP that labels two types of cells in the retina: the amacrine and the horizontal cells and also the hindbrain. Fusion of seven views from Lightsheet Z.1
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