DeutschEnglish

Caren Norden

Somal translocation of retinal ganglion cells visualized by the Ath5::membraneGFP transgene. Captured by the Lightsheet Z.1 microscope. Movie by Jaroslav Icha, edited by Julia Eichhorn.

Early cleavage divisions of a zebrafish embryo, where mitotic spindles are visualized by the Eef1a1::dclk2-GFP transgene. Movie captured by Jaroslav Icha and Franziska Decker during the PhD course. Imaged at the Lightsheet Z.1 microscope.

An animated z-stack of the retinal pseudotratified neuroepithelium at ~30 hpf, with plasma membranes in green, all nuclei in blue and mitotic nuclei in magenta. Note the apical-most position of all the mitotic nuclei, a result of the inter kinetic nuclear migration in G2.

 

A division of retinal neuroepithelial cell where the primary cillium is labeled by Arl13b-GFP (magenta) and the microtubule plus tips visualized by EB3-mKate2 (green).

A division of retinal neuroepithelial cell with a horizontal cleavage plane. Nuclei are labeled by H2B-RFP (green) and the apical domain is labeled by Par3-GFP (magenta).

A multiview acquired during the EMBO course in Lightsheet microscopy with Yong Wang, University of Toronto. The movie shows a transgenic line Ptf1a:GFP that labels two types of cells in the retina: the amacrine and the horizontal cells and also the hindbrain. Fusion of seven views from Lightsheet Z.1

 

Mitotic Position and Morphology of Committed Precursor Cells in the Zebrafish Retina Adapt to Architectural Changes upon Tissue Maturation. Weber et al. Cell Rep., 7, no. 1, pp. 1-12, (2014)
The retina of a 3 day old zebrafish embryo expressing Ath5::RFP transgene (magenta) with antibody stained acetylated microtubules (green). This image was selected for the annual exhibition Images of Science of the Max Planck Society
Graphical abstract of (Weber et al., 2014) describing comitted precursors in the zebrafish retina and their nonapical divisions.
"Feather in the cap ": The neuroepithelial cells bear primary cilia at their apical side. Zebrafish neuroepithelium immuno-stained for Acetylated tubulin (magenta), Centrin (green) and nuclei (Gray)
"Caught in the act": Apically dividing neuroepithelial cells showing various stages of mitosis. Mitotic nuclei can be distinguished by condensed chromatin staining. The developing retinal neuroepithelium was stained with DAPI.
A figure from the review about neuronal migration (Icha and Norden, 2014) showing tangential (A) and radial (B) migration, graphics by Franziska Friedrich.
The model of retinal development from (Weber et al., 2014) showing the newly described committed precursor cells, some of which divide in non-apical locations in the retina.
The cis and trans-Golgi apparatus labeled in four cells of the zebrafish larval epidermis.
Quantitative analysis of cell cycle dependent nuclear movement from (Leung et al., 2011).
The zebrafish embryo at around 32 hours after fertilization.