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Microarray Analysis

Microarray Analysis

The Microarray Facility currently provides service for three different commercially available DNA microarray platforms: Agilent, Affymetrix and NimbleGen. The Agilent hybridization oven and microarray confocal laser scanner set-up of the Microarray Facility allows for analysis of other 1x3” glass slide array platforms, also.  Such platforms include but are not limited to, GE Healthcare, Illumina, or custom printed arrays.  Any type of microarray, e.g. protein, peptide, antibody, lipid, glycan, etc. can be processed in the Microarray Facility.  We have expertise in designing custom microarrays, for example DNA Microarrays with the Agilent database eArray. We offer full service of microarray experiments including sample quality control, experimental design, execution of the experiments, data analysis and data storage. For the validation of DNA microarray results, we are equipped and experienced with qPCR analysis.

Services

  • Gene expression analysis (1 color and 2 color)
  • ChIP-on-chip analysis
  • Array-based comparative genomic hybridization
  • miRNA expression analysis
  • Array-based and solution-based targeted enrichment for Next Generation Sequencing
  • Any type of array processing, e.g. protein, peptide, antibody, lipid, glycan, etc.
  • Validation of DNA microarray results with qPCR
  • Custom array design, e.g. with eArray tool

Expertise

  • Comprehensive microarray proficiency
  • Professional support, teaching, and training
  • High throughput screening and large-scale data analysis
  • Experimental design and assay development
  • Design and analysis of custom microarrays, such as DNA, protein, peptide, antibody, lipid, glycan, etc.

Equipment

  • Agilent microarray confocal laser scanner
  • Agilent hybridization oven
  • Agilent Bioanalyzer
  • NimbleGen hybridization system 4
  • Stratagene Mx 3000 P qPCR machines
  • Large-scale data analysis platforms: GeneSpring; Partek